Experiment set7S141 for Escherichia coli BL21
Bas42
Group: phageMedia: LB_plus_SM_buffer + MOI 10
Culturing: BL21_ML4, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 01/19/2024
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 13 genes in this experiment
For phage MOI 10 in Escherichia coli BL21
For phage MOI 10 across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycolysis / Gluconeogenesis
- Fructose and mannose metabolism
- Galactose metabolism
- Ascorbate and aldarate metabolism
- Alanine and aspartate metabolism
- Glycine, serine and threonine metabolism
- Cyanoamino acid metabolism
- Starch and sucrose metabolism
- Nucleotide sugars metabolism
- Aminosugars metabolism
- Nitrogen metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-asparagine degradation I | 1 | 1 | 1 |
| glycine biosynthesis II | 3 | 3 | 1 |
| glycine cleavage | 3 | 3 | 1 |
| L-asparagine degradation III (mammalian) | 3 | 2 | 1 |
| superpathway of L-aspartate and L-asparagine biosynthesis | 4 | 4 | 1 |
| NAD salvage pathway II (PNC IV cycle) | 5 | 4 | 1 |
| N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation | 5 | 2 | 1 |