Experiment set7IT084 for Pseudomonas syringae pv. syringae B728a ΔmexB

KB with Palmatine 10 mM

Group: stress
Media: KB + Palmatine (10 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: SyringaeB728a_mexBdelta_ML3, 24 well microplate, Aerobic, at 28 (C), shaken=250 rpm
By: Tyler Helmann on 7/23/19
Media components: 10 g/L Bacto Peptone, 1.5 g/L Potassium phosphate dibasic, 15 g/L Glycerol, 0.6 g/L Magnesium sulfate

Specific Phenotypes

For 9 genes in this experiment

For stress Palmatine in Pseudomonas syringae pv. syringae B728a ΔmexB

For stress Palmatine across organisms

SEED Subsystems

Subsystem	#Specific
Multidrug Resistance Efflux Pumps	2
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	2
Arginine and Ornithine Degradation	1
Glutathione-dependent pathway of formaldehyde detoxification	1
Polyamine Metabolism	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Urea cycle and metabolism of amino groups
• Arginine and proline metabolism
• Pyrimidine metabolism
• Methane metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway)	3	3	2
putrescine biosynthesis II	3	3	2
pseudouridine degradation	2	1	1
formaldehyde oxidation II (glutathione-dependent)	3	3	1
methylglyoxal degradation I	3	2	1
methylglyoxal degradation VIII	3	2	1
superpathway of polyamine biosynthesis II	8	7	2
putrescine degradation II	4	1	1
superpathway of ornithine degradation	8	4	1
superpathway of methylglyoxal degradation	8	4	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	5	1
superpathway of C1 compounds oxidation to CO2	12	5	1
superpathway of L-arginine and L-ornithine degradation	13	7	1