Experiment set7IT078 for Escherichia coli BW25113

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Dodecanol carbon source

Group: carbon source
Media: M9 minimal media_noCarbon + Dodecanol (10 mM)
Culturing: Keio_ML9a, tube, Aerobic, at 37 (C), shaken=200 rpm
By: Ankita Kothari on 7/20/2015
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 4 genes in this experiment

For carbon source Dodecanol in Escherichia coli BW25113

For carbon source Dodecanol across organisms

SEED Subsystems

Subsystem #Specific
Acetyl-CoA fermentation to Butyrate 2
Butanol Biosynthesis 2
Isoleucine degradation 2
Valine degradation 2
n-Phenylalkanoic acid degradation 2
Anaerobic respiratory reductases 1
Biotin biosynthesis 1
D-gluconate and ketogluconates metabolism 1
Isobutyryl-CoA to Propionyl-CoA Module 1
Polyhydroxybutyrate metabolism 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
long-chain fatty acid activation 1 1 1
fatty acid β-oxidation III (unsaturated, odd number) 1 1 1
fatty acid β-oxidation I (generic) 7 6 5
benzoyl-CoA biosynthesis 3 3 2
fatty acid β-oxidation IV (unsaturated, even number) 5 3 3
oleate β-oxidation 35 32 20
oleate β-oxidation (thioesterase-dependent, yeast) 2 2 1
fatty acid salvage 6 5 3
pyruvate fermentation to butanol II (engineered) 6 4 3
γ-linolenate biosynthesis II (animals) 2 1 1
linoleate biosynthesis II (animals) 2 1 1
cinnamoyl-CoA biosynthesis 2 1 1
pyruvate fermentation to hexanol (engineered) 11 8 5
adipate degradation 5 5 2
adipate biosynthesis 5 4 2
fatty acid β-oxidation II (plant peroxisome) 5 3 2
glutaryl-CoA degradation 5 3 2
benzoate biosynthesis III (CoA-dependent, non-β-oxidative) 5 2 2
fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) 5 2 2
(8E,10E)-dodeca-8,10-dienol biosynthesis 11 5 4
2-methyl-branched fatty acid β-oxidation 14 9 5
L-isoleucine degradation I 6 4 2
valproate β-oxidation 9 5 3
propanoate fermentation to 2-methylbutanoate 6 3 2
3-methyl-branched fatty acid α-oxidation 6 3 2
methyl ketone biosynthesis (engineered) 6 3 2
benzoate biosynthesis I (CoA-dependent, β-oxidative) 9 4 3
6-gingerol analog biosynthesis (engineered) 6 2 2
oleate β-oxidation (reductase-dependent, yeast) 3 1 1
oleate biosynthesis I (plants) 3 1 1
alkane biosynthesis II 3 1 1
3-phenylpropanoate degradation 10 4 3
pyruvate fermentation to butanoate 7 4 2
fatty acid β-oxidation VI (mammalian peroxisome) 7 3 2
benzoyl-CoA degradation I (aerobic) 7 3 2
phytol degradation 4 3 1
L-valine degradation I 8 4 2
pyruvate fermentation to butanol I 8 4 2
phosphatidylcholine acyl editing 4 2 1
pinosylvin metabolism 4 1 1
oleate β-oxidation (isomerase-dependent, yeast) 4 1 1
wax esters biosynthesis II 4 1 1
long chain fatty acid ester synthesis (engineered) 4 1 1
phenylacetate degradation I (aerobic) 9 9 2
superpathway of Clostridium acetobutylicum acidogenic fermentation 9 6 2
sporopollenin precursors biosynthesis 18 5 4
5,6-dehydrokavain biosynthesis (engineered) 10 8 2
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) 5 4 1
L-glutamate degradation V (via hydroxyglutarate) 10 5 2
sphingosine and sphingosine-1-phosphate metabolism 10 4 2
9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) 10 4 2
octane oxidation 5 2 1
4-hydroxybenzoate biosynthesis III (plants) 5 2 1
superpathway of phenylethylamine degradation 11 11 2
stearate biosynthesis II (bacteria and plants) 6 5 1
stearate biosynthesis IV 6 4 1
L-glutamate degradation VII (to butanoate) 12 5 2
stearate biosynthesis I (animals) 6 1 1
superpathway of Clostridium acetobutylicum solventogenic fermentation 13 7 2
superpathway of glyoxylate cycle and fatty acid degradation 14 11 2
ceramide degradation by α-oxidation 7 2 1
capsaicin biosynthesis 7 1 1
icosapentaenoate biosynthesis III (8-desaturase, mammals) 7 1 1
icosapentaenoate biosynthesis II (6-desaturase, mammals) 7 1 1
arachidonate biosynthesis III (6-desaturase, mammals) 7 1 1
Spodoptera littoralis pheromone biosynthesis 22 3 3
L-tryptophan degradation III (eukaryotic) 15 3 2
glycerol degradation to butanol 16 11 2
2-deoxy-D-ribose degradation II 8 3 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 2
2-methylpropene degradation 8 2 1
crotonate fermentation (to acetate and cyclohexane carboxylate) 16 3 2
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 9 2
benzoate fermentation (to acetate and cyclohexane carboxylate) 17 3 2
1-butanol autotrophic biosynthesis (engineered) 27 19 3
3-hydroxypropanoate/4-hydroxybutanate cycle 18 10 2
toluene degradation VI (anaerobic) 18 3 2
methyl tert-butyl ether degradation 10 2 1
suberin monomers biosynthesis 20 3 2
superpathway of fatty acid biosynthesis II (plant) 43 38 4
gallate degradation III (anaerobic) 11 5 1
10-cis-heptadecenoyl-CoA degradation (yeast) 12 2 1
10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) 12 2 1
androstenedione degradation I (aerobic) 25 6 2
platensimycin biosynthesis 26 6 2
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) 13 2 1
androstenedione degradation II (anaerobic) 27 4 2
superpathway of testosterone and androsterone degradation 28 6 2
superpathway of cholesterol degradation I (cholesterol oxidase) 42 8 3
docosahexaenoate biosynthesis III (6-desaturase, mammals) 14 2 1
palmitate biosynthesis II (type II fatty acid synthase) 31 29 2
superpathway of cholesterol degradation II (cholesterol dehydrogenase) 47 8 3
cutin biosynthesis 16 2 1
cholesterol degradation to androstenedione I (cholesterol oxidase) 17 2 1
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) 22 2 1
superpathway of cholesterol degradation III (oxidase) 49 4 2
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 19 1
superpathway of fatty acids biosynthesis (E. coli) 53 51 2
palmitate biosynthesis III 29 21 1