Experiment set7IT058 for Pseudomonas syringae pv. syringae B728a ΔmexB
KB with Lawsone 10 mM
Group: stressMedia: KB + Lawsone (10 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: SyringaeB728a_mexBdelta_ML3, 24 well microplate, Aerobic, at 28 (C), shaken=250 rpm
By: Tyler Helmann on 7/23/19
Media components: 10 g/L Bacto Peptone, 1.5 g/L Potassium phosphate dibasic, 15 g/L Glycerol, 0.6 g/L Magnesium sulfate
Specific Phenotypes
For 2 genes in this experiment
For stress Lawsone in Pseudomonas syringae pv. syringae B728a ΔmexB
For stress Lawsone across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Biogenesis of cytochrome c oxidases | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| photosynthesis light reactions | 4 | 1 | 1 |
| NADPH to cytochrome c oxidase via plastocyanin (thylakoid membrane) | 4 | 1 | 1 |
| superpathway of photosynthetic hydrogen production | 6 | 2 | 1 |
| oxygenic photosynthesis | 17 | 11 | 1 |
| ethene biosynthesis V (engineered) | 25 | 18 | 1 |
| photosynthetic 3-hydroxybutanoate biosynthesis (engineered) | 26 | 19 | 1 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 19 | 1 |