Experiment set7IT057 for Pseudomonas putida KT2440

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5-Aminovaleric acid 10 mM carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + 5-Aminovaleric acid (10 mM)
Culturing: Putida_ML5_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 6.5 generations
By: Mitchell Thompson on 4/26/18
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 9 genes in this experiment

For carbon source 5-Aminovaleric acid in Pseudomonas putida KT2440

For carbon source 5-Aminovaleric acid across organisms

SEED Subsystems

Subsystem #Specific
Cobalt-zinc-cadmium resistance 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
glutarate degradation 2 2 2
4-aminobutanoate degradation III 2 2 1
L-lysine degradation IV 5 5 2
L-lysine degradation X 6 5 2
L-lysine degradation I 7 5 2
L-lysine degradation III 6 2 1
photorespiration I 9 6 1
superpathway of L-lysine degradation 43 23 3