Experiment set7IT048 for Pseudomonas putida KT2440

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3-methyl-1-butanol 0.1 vol% carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + 3-methyl-1-butanol (0.1 vol%)
Culturing: Putida_ML5_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 5.6 generations
By: Mitchell Thompson on 4/25/18
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 78 genes in this experiment

For carbon source 3-methyl-1-butanol in Pseudomonas putida KT2440

For carbon source 3-methyl-1-butanol across organisms

SEED Subsystems

Subsystem #Specific
Aromatic amino acid degradation 4
Iron acquisition in Vibrio 4
ABC transporter dipeptide (TC 3.A.1.5.2) 3
Arginine and Ornithine Degradation 3
Leucine Degradation and HMG-CoA Metabolism 3
Multidrug Resistance Efflux Pumps 3
Thioredoxin-disulfide reductase 3
Transport of Iron 3
Campylobacter Iron Metabolism 2
Choline and Betaine Uptake and Betaine Biosynthesis 2
Cysteine Biosynthesis 2
DNA-binding regulatory proteins, strays 2
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon) 2
Oxidative stress 2
Phosphate metabolism 2
Photorespiration (oxidative C2 cycle) 2
Polyamine Metabolism 2
Rubrerythrin 2
Serine-glyoxylate cycle 2
Ton and Tol transport systems 2
cAMP signaling in bacteria 2
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 1
Acid resistance mechanisms 1
Auxin degradation 1
Bacterial Chemotaxis 1
Biogenesis of cytochrome c oxidases 1
Biotin biosynthesis 1
Calvin-Benson cycle 1
Catechol branch of beta-ketoadipate pathway 1
Copper homeostasis 1
Cyanate hydrolysis 1
DNA repair, bacterial MutL-MutS system 1
De Novo Pyrimidine Synthesis 1
Entner-Doudoroff Pathway 1
Fermentations: Mixed acid 1
Flavodoxin 1
Folate Biosynthesis 1
Glutathione-dependent pathway of formaldehyde detoxification 1
Glutathione: Redox cycle 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
Glycine and Serine Utilization 1
Glycine cleavage system 1
Glycolysis and Gluconeogenesis 1
HMG CoA Synthesis 1
Isoleucine degradation 1
Ketoisovalerate oxidoreductase 1
LMPTP YwlE cluster 1
Lactate utilization 1
Lysine degradation 1
Methionine Biosynthesis 1
Proteasome bacterial 1
Protein degradation 1
Proteolysis in bacteria, ATP-dependent 1
Protocatechuate branch of beta-ketoadipate pathway 1
Pterin biosynthesis 1
Purine conversions 1
Pyridoxin (Vitamin B6) Biosynthesis 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 1
Redox-dependent regulation of nucleus processes 1
Ribosome biogenesis bacterial 1
Sialic Acid Metabolism 1
Valine degradation 1
n-Phenylalkanoic acid degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
acetate and ATP formation from acetyl-CoA III 1 1 1
pyrimidine nucleobases salvage I 1 1 1
L-glutamine biosynthesis I 1 1 1
long-chain fatty acid activation 1 1 1
L-phenylalanine degradation I (aerobic) 1 1 1
acetate conversion to acetyl-CoA 1 1 1
betanidin degradation 1 1 1
L-tyrosine biosynthesis IV 1 1 1
arginine dependent acid resistance 1 1 1
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway) 3 3 2
putrescine biosynthesis II 3 3 2
ethanol degradation IV 3 3 2
urea degradation I 3 2 2
L-cysteine biosynthesis I 2 2 1
guanine and guanosine salvage I 2 2 1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway) 2 2 1
guanine and guanosine salvage II 2 2 1
CO2 fixation into oxaloacetate (anaplerotic) 2 2 1
adenine and adenosine salvage I 2 2 1
putrescine biosynthesis I 2 2 1
ammonia assimilation cycle II 2 2 1
superoxide radicals degradation 2 2 1
ammonia assimilation cycle I 2 2 1
pyrimidine nucleobases salvage II 2 2 1
adenine and adenosine salvage II 2 2 1
methanol oxidation to formaldehyde IV 2 1 1
phospholipid remodeling (phosphatidate, yeast) 2 1 1
linoleate biosynthesis II (animals) 2 1 1
palmitoleate biosynthesis III (cyanobacteria) 2 1 1
γ-linolenate biosynthesis II (animals) 2 1 1
baicalein degradation (hydrogen peroxide detoxification) 2 1 1
octane oxidation 5 4 2
cyanuric acid degradation II 5 3 2
cyanuric acid degradation I 5 2 2
2-oxoisovalerate decarboxylation to isobutanoyl-CoA 3 3 1
dTMP de novo biosynthesis (mitochondrial) 3 3 1
glycine biosynthesis II 3 3 1
L-phenylalanine degradation V 3 3 1
formaldehyde oxidation II (glutathione-dependent) 3 3 1
tetrahydrofolate biosynthesis I 3 3 1
ketolysis 3 3 1
glycine cleavage 3 3 1
ethanol degradation II 3 3 1
superpathway of ammonia assimilation (plants) 3 3 1
L-citrulline degradation 3 3 1
superpathway of acetate utilization and formation 3 3 1
ammonia assimilation cycle III 3 3 1
L-leucine degradation I 6 5 2
ethanol degradation III 3 2 1
cyanate degradation 3 2 1
L-isoleucine biosynthesis V 3 2 1
superpathway of guanine and guanosine salvage 3 2 1
oleate biosynthesis III (cyanobacteria) 3 2 1
methylglyoxal degradation VIII 3 2 1
glutathione-peroxide redox reactions 3 2 1
L-aspartate degradation III (anaerobic) 3 2 1
L-aspartate degradation II (aerobic) 3 2 1
methylglyoxal degradation I 3 2 1
adenine salvage 3 2 1
superpathway of allantoin degradation in yeast 6 3 2
3-methyl-branched fatty acid α-oxidation 6 3 2
gentisate degradation I 3 1 1
oleate biosynthesis I (plants) 3 1 1
alkane biosynthesis II 3 1 1
CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis 3 1 1
reactive oxygen species degradation 4 4 1
queuosine biosynthesis I (de novo) 4 4 1
CDP-diacylglycerol biosynthesis II 4 4 1
L-arginine degradation V (arginine deiminase pathway) 4 4 1
superpathway of pyrimidine nucleobases salvage 4 4 1
CDP-diacylglycerol biosynthesis I 4 4 1
glycine betaine degradation I 8 6 2
phytol degradation 4 3 1
superpathway of putrescine biosynthesis 4 3 1
superpathway of polyamine biosynthesis II 8 5 2
creatinine degradation I 4 2 1
chitin deacetylation 4 2 1
superpathway of atrazine degradation 8 3 2
wax esters biosynthesis II 4 1 1
luteolin triglucuronide degradation 4 1 1
phosphatidylcholine acyl editing 4 1 1
long chain fatty acid ester synthesis (engineered) 4 1 1
spermidine biosynthesis III 4 1 1
4-hydroxy-2-nonenal detoxification 4 1 1
xanthommatin biosynthesis 4 1 1
sporopollenin precursors biosynthesis 18 4 4
2-methylcitrate cycle I 5 5 1
L-tyrosine degradation I 5 5 1
CDP-diacylglycerol biosynthesis III 5 3 1
phosphatidate biosynthesis (yeast) 5 3 1
queuosine biosynthesis III (queuosine salvage) 5 3 1
uracil degradation III 5 3 1
creatinine degradation II 5 3 1
seleno-amino acid biosynthesis (plants) 5 3 1
pentachlorophenol degradation 10 4 2
sphingosine and sphingosine-1-phosphate metabolism 10 4 2
phosphatidylglycerol biosynthesis II 6 6 1
fatty acid salvage 6 6 1
phosphatidylglycerol biosynthesis I 6 6 1
superpathway of phospholipid biosynthesis III (E. coli) 12 10 2
2-methylcitrate cycle II 6 5 1
stearate biosynthesis II (bacteria and plants) 6 5 1
β-alanine biosynthesis II 6 5 1
L-isoleucine biosynthesis IV 6 4 1
stearate biosynthesis IV 6 4 1
6-gingerol analog biosynthesis (engineered) 6 3 1
5-nitroanthranilate degradation 6 2 1
palmitoyl ethanolamide biosynthesis 6 2 1
superpathway of stearidonate biosynthesis (cyanobacteria) 6 2 1
superpathway of bitter acids biosynthesis 18 3 3
adlupulone and adhumulone biosynthesis 6 1 1
lupulone and humulone biosynthesis 6 1 1
stearate biosynthesis I (animals) 6 1 1
hydrogen sulfide biosynthesis II (mammalian) 6 1 1
colupulone and cohumulone biosynthesis 6 1 1
pyridoxal 5'-phosphate biosynthesis I 7 7 1
superpathway of purine nucleotide salvage 14 13 2
L-glutamate and L-glutamine biosynthesis 7 6 1
C4 photosynthetic carbon assimilation cycle, NADP-ME type 7 4 1
glyphosate degradation III 7 3 1
capsaicin biosynthesis 7 3 1
diacylglycerol and triacylglycerol biosynthesis 7 3 1
ceramide degradation by α-oxidation 7 2 1
stigma estolide biosynthesis 7 2 1
icosapentaenoate biosynthesis III (8-desaturase, mammals) 7 1 1
arachidonate biosynthesis III (6-desaturase, mammals) 7 1 1
icosapentaenoate biosynthesis II (6-desaturase, mammals) 7 1 1
partial TCA cycle (obligate autotrophs) 8 8 1
nitrogen remobilization from senescing leaves 8 6 1
superpathway of polyamine biosynthesis I 8 5 1
superpathway of methylglyoxal degradation 8 5 1
2-deoxy-D-ribose degradation II 8 4 1
glutathione-mediated detoxification I 8 3 1
(aminomethyl)phosphonate degradation 8 3 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 2
anandamide biosynthesis II 8 2 1
sesamin biosynthesis 8 1 1
superpathway of CDP-glucose-derived O-antigen building blocks biosynthesis 8 1 1
superpathway of sulfate assimilation and cysteine biosynthesis 9 9 1
folate transformations III (E. coli) 9 9 1
Entner-Doudoroff pathway I 9 8 1
sucrose biosynthesis I (from photosynthesis) 9 7 1
TCA cycle VI (Helicobacter) 9 7 1
reductive glycine pathway of autotrophic CO2 fixation 9 5 1
cis-geranyl-CoA degradation 9 2 1
gliotoxin biosynthesis 9 2 1
allantoin degradation IV (anaerobic) 9 2 1
glutathione-mediated detoxification II 9 1 1
L-arginine biosynthesis II (acetyl cycle) 10 10 1
superpathway of coenzyme A biosynthesis II (plants) 10 9 1
superpathway of tetrahydrofolate biosynthesis 10 8 1
glycolysis IV 10 8 1
superpathway of pyrimidine ribonucleosides salvage 10 7 1
3-phenylpropanoate degradation 10 4 1
suberin monomers biosynthesis 20 4 2
matairesinol biosynthesis 10 1 1
justicidin B biosynthesis 10 1 1
superpathway of fatty acid biosynthesis II (plant) 43 38 4
folate transformations II (plants) 11 10 1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 9 1
glycolysis III (from glucose) 11 9 1
glycolysis II (from fructose 6-phosphate) 11 9 1
glycolysis VI (from fructose) 11 7 1
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 7 1
superpathway of pyridoxal 5'-phosphate biosynthesis and salvage 12 11 1
formaldehyde assimilation III (dihydroxyacetone cycle) 12 10 1
superpathway of tetrahydrofolate biosynthesis and salvage 12 10 1
gluconeogenesis III 12 9 1
homolactic fermentation 12 9 1
superpathway of C1 compounds oxidation to CO2 12 5 1
anandamide biosynthesis I 12 3 1
indole glucosinolate activation (intact plant cell) 12 3 1
camalexin biosynthesis 12 2 1
gluconeogenesis I 13 11 1
superpathway of L-arginine and L-ornithine degradation 13 11 1
glycolysis I (from glucose 6-phosphate) 13 10 1
superpathway of cardiolipin biosynthesis (bacteria) 13 9 1
formaldehyde assimilation I (serine pathway) 13 7 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 8 1
superpathway of phospholipid biosynthesis II (plants) 28 10 2
Bifidobacterium shunt 15 12 1
palmitate biosynthesis II (type II fatty acid synthase) 31 29 2
mixed acid fermentation 16 12 1
glycerol degradation to butanol 16 9 1
cutin biosynthesis 16 1 1
plasmalogen biosynthesis I (aerobic) 16 1 1
superpathway of glucose and xylose degradation 17 14 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 14 1
superpathway of arginine and polyamine biosynthesis 17 14 1
superpathway of hexitol degradation (bacteria) 18 13 1
heterolactic fermentation 18 12 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 9 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 14 1
superpathway of anaerobic sucrose degradation 19 13 1
superpathway of seleno-compound metabolism 19 8 1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle 22 18 1
superpathway of N-acetylneuraminate degradation 22 12 1
ethene biosynthesis V (engineered) 25 18 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 22 1
superpathway of fatty acids biosynthesis (E. coli) 53 51 2
palmitate biosynthesis III 29 28 1
oleate β-oxidation 35 30 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 21 1
superpathway of chorismate metabolism 59 42 1