Experiment set7IT048 for Pseudomonas putida KT2440

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3-methyl-1-butanol 0.1 vol% carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + 3-methyl-1-butanol (0.1 vol%)
Culturing: Putida_ML5_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 5.6 generations
By: Mitchell Thompson on 4/25/18
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 78 genes in this experiment

For carbon source 3-methyl-1-butanol in Pseudomonas putida KT2440

For carbon source 3-methyl-1-butanol across organisms

SEED Subsystems

Subsystem #Specific
Aromatic amino acid degradation 4
Iron acquisition in Vibrio 4
ABC transporter dipeptide (TC 3.A.1.5.2) 3
Arginine and Ornithine Degradation 3
Leucine Degradation and HMG-CoA Metabolism 3
Multidrug Resistance Efflux Pumps 3
Thioredoxin-disulfide reductase 3
Transport of Iron 3
Campylobacter Iron Metabolism 2
Choline and Betaine Uptake and Betaine Biosynthesis 2
Cysteine Biosynthesis 2
DNA-binding regulatory proteins, strays 2
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon) 2
Oxidative stress 2
Phosphate metabolism 2
Photorespiration (oxidative C2 cycle) 2
Polyamine Metabolism 2
Rubrerythrin 2
Serine-glyoxylate cycle 2
Ton and Tol transport systems 2
cAMP signaling in bacteria 2
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 1
Acid resistance mechanisms 1
Auxin degradation 1
Bacterial Chemotaxis 1
Biogenesis of cytochrome c oxidases 1
Biotin biosynthesis 1
Calvin-Benson cycle 1
Catechol branch of beta-ketoadipate pathway 1
Copper homeostasis 1
Cyanate hydrolysis 1
DNA repair, bacterial MutL-MutS system 1
De Novo Pyrimidine Synthesis 1
Entner-Doudoroff Pathway 1
Fermentations: Mixed acid 1
Flavodoxin 1
Folate Biosynthesis 1
Glutathione-dependent pathway of formaldehyde detoxification 1
Glutathione: Redox cycle 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
Glycine and Serine Utilization 1
Glycine cleavage system 1
Glycolysis and Gluconeogenesis 1
HMG CoA Synthesis 1
Isoleucine degradation 1
Ketoisovalerate oxidoreductase 1
LMPTP YwlE cluster 1
Lactate utilization 1
Lysine degradation 1
Methionine Biosynthesis 1
Proteasome bacterial 1
Protein degradation 1
Proteolysis in bacteria, ATP-dependent 1
Protocatechuate branch of beta-ketoadipate pathway 1
Pterin biosynthesis 1
Purine conversions 1
Pyridoxin (Vitamin B6) Biosynthesis 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 1
Redox-dependent regulation of nucleus processes 1
Ribosome biogenesis bacterial 1
Sialic Acid Metabolism 1
Valine degradation 1
n-Phenylalkanoic acid degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
acetate conversion to acetyl-CoA 1 1 1
long-chain fatty acid activation 1 1 1
acetate and ATP formation from acetyl-CoA III 1 1 1
L-tyrosine biosynthesis IV 1 1 1
pyrimidine nucleobases salvage I 1 1 1
L-glutamine biosynthesis I 1 1 1
L-phenylalanine degradation I (aerobic) 1 1 1
arginine dependent acid resistance 1 1 1
betanidin degradation 1 1 1
putrescine biosynthesis II 3 3 2
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway) 3 3 2
ethanol degradation IV 3 3 2
urea degradation I 3 2 2
pyrimidine nucleobases salvage II 2 2 1
superoxide radicals degradation 2 2 1
adenine and adenosine salvage I 2 2 1
CO2 fixation into oxaloacetate (anaplerotic) 2 2 1
adenine and adenosine salvage II 2 2 1
guanine and guanosine salvage I 2 2 1
guanine and guanosine salvage II 2 2 1
putrescine biosynthesis I 2 2 1
L-cysteine biosynthesis I 2 2 1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway) 2 2 1
ammonia assimilation cycle I 2 2 1
ammonia assimilation cycle II 2 2 1
methanol oxidation to formaldehyde IV 2 1 1
γ-linolenate biosynthesis II (animals) 2 1 1
phospholipid remodeling (phosphatidate, yeast) 2 1 1
baicalein degradation (hydrogen peroxide detoxification) 2 1 1
palmitoleate biosynthesis III (cyanobacteria) 2 1 1
linoleate biosynthesis II (animals) 2 1 1
octane oxidation 5 4 2
cyanuric acid degradation II 5 3 2
cyanuric acid degradation I 5 2 2
glycine biosynthesis II 3 3 1
formaldehyde oxidation II (glutathione-dependent) 3 3 1
dTMP de novo biosynthesis (mitochondrial) 3 3 1
superpathway of acetate utilization and formation 3 3 1
ketolysis 3 3 1
ammonia assimilation cycle III 3 3 1
glycine cleavage 3 3 1
superpathway of ammonia assimilation (plants) 3 3 1
ethanol degradation II 3 3 1
2-oxoisovalerate decarboxylation to isobutanoyl-CoA 3 3 1
tetrahydrofolate biosynthesis I 3 3 1
L-phenylalanine degradation V 3 3 1
L-citrulline degradation 3 3 1
L-leucine degradation I 6 5 2
L-isoleucine biosynthesis V 3 2 1
adenine salvage 3 2 1
superpathway of guanine and guanosine salvage 3 2 1
L-aspartate degradation II (aerobic) 3 2 1
glutathione-peroxide redox reactions 3 2 1
L-aspartate degradation III (anaerobic) 3 2 1
methylglyoxal degradation VIII 3 2 1
ethanol degradation III 3 2 1
cyanate degradation 3 2 1
oleate biosynthesis III (cyanobacteria) 3 2 1
methylglyoxal degradation I 3 2 1
3-methyl-branched fatty acid α-oxidation 6 3 2
superpathway of allantoin degradation in yeast 6 3 2
CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis 3 1 1
alkane biosynthesis II 3 1 1
gentisate degradation I 3 1 1
oleate biosynthesis I (plants) 3 1 1
CDP-diacylglycerol biosynthesis II 4 4 1
L-arginine degradation V (arginine deiminase pathway) 4 4 1
CDP-diacylglycerol biosynthesis I 4 4 1
queuosine biosynthesis I (de novo) 4 4 1
reactive oxygen species degradation 4 4 1
superpathway of pyrimidine nucleobases salvage 4 4 1
glycine betaine degradation I 8 6 2
phytol degradation 4 3 1
superpathway of putrescine biosynthesis 4 3 1
superpathway of polyamine biosynthesis II 8 5 2
chitin deacetylation 4 2 1
creatinine degradation I 4 2 1
superpathway of atrazine degradation 8 3 2
long chain fatty acid ester synthesis (engineered) 4 1 1
luteolin triglucuronide degradation 4 1 1
xanthommatin biosynthesis 4 1 1
phosphatidylcholine acyl editing 4 1 1
spermidine biosynthesis III 4 1 1
wax esters biosynthesis II 4 1 1
4-hydroxy-2-nonenal detoxification 4 1 1
sporopollenin precursors biosynthesis 18 4 4
L-tyrosine degradation I 5 5 1
2-methylcitrate cycle I 5 5 1
creatinine degradation II 5 3 1
seleno-amino acid biosynthesis (plants) 5 3 1
queuosine biosynthesis III (queuosine salvage) 5 3 1
uracil degradation III 5 3 1
CDP-diacylglycerol biosynthesis III 5 3 1
phosphatidate biosynthesis (yeast) 5 3 1
sphingosine and sphingosine-1-phosphate metabolism 10 4 2
pentachlorophenol degradation 10 4 2
phosphatidylglycerol biosynthesis I 6 6 1
phosphatidylglycerol biosynthesis II 6 6 1
fatty acid salvage 6 6 1
superpathway of phospholipid biosynthesis III (E. coli) 12 10 2
β-alanine biosynthesis II 6 5 1
2-methylcitrate cycle II 6 5 1
stearate biosynthesis II (bacteria and plants) 6 5 1
stearate biosynthesis IV 6 4 1
L-isoleucine biosynthesis IV 6 4 1
6-gingerol analog biosynthesis (engineered) 6 3 1
palmitoyl ethanolamide biosynthesis 6 2 1
5-nitroanthranilate degradation 6 2 1
superpathway of stearidonate biosynthesis (cyanobacteria) 6 2 1
superpathway of bitter acids biosynthesis 18 3 3
stearate biosynthesis I (animals) 6 1 1
colupulone and cohumulone biosynthesis 6 1 1
hydrogen sulfide biosynthesis II (mammalian) 6 1 1
adlupulone and adhumulone biosynthesis 6 1 1
lupulone and humulone biosynthesis 6 1 1
pyridoxal 5'-phosphate biosynthesis I 7 7 1
superpathway of purine nucleotide salvage 14 13 2
L-glutamate and L-glutamine biosynthesis 7 6 1
C4 photosynthetic carbon assimilation cycle, NADP-ME type 7 4 1
diacylglycerol and triacylglycerol biosynthesis 7 3 1
glyphosate degradation III 7 3 1
capsaicin biosynthesis 7 3 1
ceramide degradation by α-oxidation 7 2 1
stigma estolide biosynthesis 7 2 1
icosapentaenoate biosynthesis III (8-desaturase, mammals) 7 1 1
icosapentaenoate biosynthesis II (6-desaturase, mammals) 7 1 1
arachidonate biosynthesis III (6-desaturase, mammals) 7 1 1
partial TCA cycle (obligate autotrophs) 8 8 1
nitrogen remobilization from senescing leaves 8 6 1
superpathway of methylglyoxal degradation 8 5 1
superpathway of polyamine biosynthesis I 8 5 1
2-deoxy-D-ribose degradation II 8 4 1
glutathione-mediated detoxification I 8 3 1
(aminomethyl)phosphonate degradation 8 3 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 2
anandamide biosynthesis II 8 2 1
superpathway of CDP-glucose-derived O-antigen building blocks biosynthesis 8 1 1
sesamin biosynthesis 8 1 1
superpathway of sulfate assimilation and cysteine biosynthesis 9 9 1
folate transformations III (E. coli) 9 9 1
Entner-Doudoroff pathway I 9 8 1
TCA cycle VI (Helicobacter) 9 7 1
sucrose biosynthesis I (from photosynthesis) 9 7 1
reductive glycine pathway of autotrophic CO2 fixation 9 5 1
allantoin degradation IV (anaerobic) 9 2 1
gliotoxin biosynthesis 9 2 1
cis-geranyl-CoA degradation 9 2 1
glutathione-mediated detoxification II 9 1 1
L-arginine biosynthesis II (acetyl cycle) 10 10 1
superpathway of coenzyme A biosynthesis II (plants) 10 9 1
glycolysis IV 10 8 1
superpathway of tetrahydrofolate biosynthesis 10 8 1
superpathway of pyrimidine ribonucleosides salvage 10 7 1
3-phenylpropanoate degradation 10 4 1
suberin monomers biosynthesis 20 4 2
matairesinol biosynthesis 10 1 1
justicidin B biosynthesis 10 1 1
superpathway of fatty acid biosynthesis II (plant) 43 38 4
folate transformations II (plants) 11 10 1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 9 1
glycolysis II (from fructose 6-phosphate) 11 9 1
glycolysis III (from glucose) 11 9 1
glycolysis VI (from fructose) 11 7 1
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 7 1
superpathway of pyridoxal 5'-phosphate biosynthesis and salvage 12 11 1
formaldehyde assimilation III (dihydroxyacetone cycle) 12 10 1
superpathway of tetrahydrofolate biosynthesis and salvage 12 10 1
gluconeogenesis III 12 9 1
homolactic fermentation 12 9 1
superpathway of C1 compounds oxidation to CO2 12 5 1
anandamide biosynthesis I 12 3 1
indole glucosinolate activation (intact plant cell) 12 3 1
camalexin biosynthesis 12 2 1
gluconeogenesis I 13 11 1
superpathway of L-arginine and L-ornithine degradation 13 11 1
glycolysis I (from glucose 6-phosphate) 13 10 1
superpathway of cardiolipin biosynthesis (bacteria) 13 9 1
formaldehyde assimilation I (serine pathway) 13 7 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 8 1
superpathway of phospholipid biosynthesis II (plants) 28 10 2
Bifidobacterium shunt 15 12 1
palmitate biosynthesis II (type II fatty acid synthase) 31 29 2
mixed acid fermentation 16 12 1
glycerol degradation to butanol 16 9 1
plasmalogen biosynthesis I (aerobic) 16 1 1
cutin biosynthesis 16 1 1
superpathway of glucose and xylose degradation 17 14 1
superpathway of arginine and polyamine biosynthesis 17 14 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 14 1
superpathway of hexitol degradation (bacteria) 18 13 1
heterolactic fermentation 18 12 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 9 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 14 1
superpathway of anaerobic sucrose degradation 19 13 1
superpathway of seleno-compound metabolism 19 8 1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle 22 18 1
superpathway of N-acetylneuraminate degradation 22 12 1
ethene biosynthesis V (engineered) 25 18 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 22 1
superpathway of fatty acids biosynthesis (E. coli) 53 51 2
palmitate biosynthesis III 29 28 1
oleate β-oxidation 35 30 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 21 1
superpathway of chorismate metabolism 59 42 1