Experiment set7IT048 for Pseudomonas putida KT2440

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3-methyl-1-butanol 0.1 vol% carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + 3-methyl-1-butanol (0.1 vol%)
Culturing: Putida_ML5_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 5.6 generations
By: Mitchell Thompson on 4/25/18
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 78 genes in this experiment

For carbon source 3-methyl-1-butanol in Pseudomonas putida KT2440

For carbon source 3-methyl-1-butanol across organisms

SEED Subsystems

Subsystem #Specific
Aromatic amino acid degradation 4
Iron acquisition in Vibrio 4
ABC transporter dipeptide (TC 3.A.1.5.2) 3
Arginine and Ornithine Degradation 3
Leucine Degradation and HMG-CoA Metabolism 3
Multidrug Resistance Efflux Pumps 3
Thioredoxin-disulfide reductase 3
Transport of Iron 3
Campylobacter Iron Metabolism 2
Choline and Betaine Uptake and Betaine Biosynthesis 2
Cysteine Biosynthesis 2
DNA-binding regulatory proteins, strays 2
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon) 2
Oxidative stress 2
Phosphate metabolism 2
Photorespiration (oxidative C2 cycle) 2
Polyamine Metabolism 2
Rubrerythrin 2
Serine-glyoxylate cycle 2
Ton and Tol transport systems 2
cAMP signaling in bacteria 2
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 1
Acid resistance mechanisms 1
Auxin degradation 1
Bacterial Chemotaxis 1
Biogenesis of cytochrome c oxidases 1
Biotin biosynthesis 1
Calvin-Benson cycle 1
Catechol branch of beta-ketoadipate pathway 1
Copper homeostasis 1
Cyanate hydrolysis 1
DNA repair, bacterial MutL-MutS system 1
De Novo Pyrimidine Synthesis 1
Entner-Doudoroff Pathway 1
Fermentations: Mixed acid 1
Flavodoxin 1
Folate Biosynthesis 1
Glutathione-dependent pathway of formaldehyde detoxification 1
Glutathione: Redox cycle 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
Glycine and Serine Utilization 1
Glycine cleavage system 1
Glycolysis and Gluconeogenesis 1
HMG CoA Synthesis 1
Isoleucine degradation 1
Ketoisovalerate oxidoreductase 1
LMPTP YwlE cluster 1
Lactate utilization 1
Lysine degradation 1
Methionine Biosynthesis 1
Proteasome bacterial 1
Protein degradation 1
Proteolysis in bacteria, ATP-dependent 1
Protocatechuate branch of beta-ketoadipate pathway 1
Pterin biosynthesis 1
Purine conversions 1
Pyridoxin (Vitamin B6) Biosynthesis 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 1
Redox-dependent regulation of nucleus processes 1
Ribosome biogenesis bacterial 1
Sialic Acid Metabolism 1
Valine degradation 1
n-Phenylalkanoic acid degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
betanidin degradation 1 1 1
L-phenylalanine degradation I (aerobic) 1 1 1
L-glutamine biosynthesis I 1 1 1
L-tyrosine biosynthesis IV 1 1 1
acetate conversion to acetyl-CoA 1 1 1
arginine dependent acid resistance 1 1 1
acetate and ATP formation from acetyl-CoA III 1 1 1
long-chain fatty acid activation 1 1 1
pyrimidine nucleobases salvage I 1 1 1
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway) 3 3 2
ethanol degradation IV 3 3 2
putrescine biosynthesis II 3 3 2
urea degradation I 3 2 2
CO2 fixation into oxaloacetate (anaplerotic) 2 2 1
ammonia assimilation cycle II 2 2 1
superoxide radicals degradation 2 2 1
adenine and adenosine salvage I 2 2 1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway) 2 2 1
guanine and guanosine salvage II 2 2 1
ammonia assimilation cycle I 2 2 1
pyrimidine nucleobases salvage II 2 2 1
adenine and adenosine salvage II 2 2 1
L-cysteine biosynthesis I 2 2 1
guanine and guanosine salvage I 2 2 1
putrescine biosynthesis I 2 2 1
methanol oxidation to formaldehyde IV 2 1 1
baicalein degradation (hydrogen peroxide detoxification) 2 1 1
linoleate biosynthesis II (animals) 2 1 1
palmitoleate biosynthesis III (cyanobacteria) 2 1 1
γ-linolenate biosynthesis II (animals) 2 1 1
phospholipid remodeling (phosphatidate, yeast) 2 1 1
octane oxidation 5 4 2
cyanuric acid degradation II 5 3 2
cyanuric acid degradation I 5 2 2
formaldehyde oxidation II (glutathione-dependent) 3 3 1
superpathway of acetate utilization and formation 3 3 1
ketolysis 3 3 1
L-phenylalanine degradation V 3 3 1
ammonia assimilation cycle III 3 3 1
glycine cleavage 3 3 1
L-citrulline degradation 3 3 1
glycine biosynthesis II 3 3 1
superpathway of ammonia assimilation (plants) 3 3 1
tetrahydrofolate biosynthesis I 3 3 1
2-oxoisovalerate decarboxylation to isobutanoyl-CoA 3 3 1
ethanol degradation II 3 3 1
dTMP de novo biosynthesis (mitochondrial) 3 3 1
L-leucine degradation I 6 5 2
glutathione-peroxide redox reactions 3 2 1
L-aspartate degradation III (anaerobic) 3 2 1
L-aspartate degradation II (aerobic) 3 2 1
methylglyoxal degradation I 3 2 1
superpathway of guanine and guanosine salvage 3 2 1
oleate biosynthesis III (cyanobacteria) 3 2 1
methylglyoxal degradation VIII 3 2 1
L-isoleucine biosynthesis V 3 2 1
cyanate degradation 3 2 1
adenine salvage 3 2 1
ethanol degradation III 3 2 1
3-methyl-branched fatty acid α-oxidation 6 3 2
superpathway of allantoin degradation in yeast 6 3 2
alkane biosynthesis II 3 1 1
CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis 3 1 1
oleate biosynthesis I (plants) 3 1 1
gentisate degradation I 3 1 1
superpathway of pyrimidine nucleobases salvage 4 4 1
queuosine biosynthesis I (de novo) 4 4 1
CDP-diacylglycerol biosynthesis II 4 4 1
CDP-diacylglycerol biosynthesis I 4 4 1
reactive oxygen species degradation 4 4 1
L-arginine degradation V (arginine deiminase pathway) 4 4 1
glycine betaine degradation I 8 6 2
phytol degradation 4 3 1
superpathway of putrescine biosynthesis 4 3 1
superpathway of polyamine biosynthesis II 8 5 2
chitin deacetylation 4 2 1
creatinine degradation I 4 2 1
superpathway of atrazine degradation 8 3 2
xanthommatin biosynthesis 4 1 1
spermidine biosynthesis III 4 1 1
phosphatidylcholine acyl editing 4 1 1
4-hydroxy-2-nonenal detoxification 4 1 1
long chain fatty acid ester synthesis (engineered) 4 1 1
luteolin triglucuronide degradation 4 1 1
wax esters biosynthesis II 4 1 1
sporopollenin precursors biosynthesis 18 4 4
2-methylcitrate cycle I 5 5 1
L-tyrosine degradation I 5 5 1
seleno-amino acid biosynthesis (plants) 5 3 1
CDP-diacylglycerol biosynthesis III 5 3 1
phosphatidate biosynthesis (yeast) 5 3 1
queuosine biosynthesis III (queuosine salvage) 5 3 1
uracil degradation III 5 3 1
creatinine degradation II 5 3 1
sphingosine and sphingosine-1-phosphate metabolism 10 4 2
pentachlorophenol degradation 10 4 2
phosphatidylglycerol biosynthesis II 6 6 1
phosphatidylglycerol biosynthesis I 6 6 1
fatty acid salvage 6 6 1
superpathway of phospholipid biosynthesis III (E. coli) 12 10 2
stearate biosynthesis II (bacteria and plants) 6 5 1
2-methylcitrate cycle II 6 5 1
β-alanine biosynthesis II 6 5 1
stearate biosynthesis IV 6 4 1
L-isoleucine biosynthesis IV 6 4 1
6-gingerol analog biosynthesis (engineered) 6 3 1
superpathway of stearidonate biosynthesis (cyanobacteria) 6 2 1
5-nitroanthranilate degradation 6 2 1
palmitoyl ethanolamide biosynthesis 6 2 1
superpathway of bitter acids biosynthesis 18 3 3
hydrogen sulfide biosynthesis II (mammalian) 6 1 1
adlupulone and adhumulone biosynthesis 6 1 1
lupulone and humulone biosynthesis 6 1 1
stearate biosynthesis I (animals) 6 1 1
colupulone and cohumulone biosynthesis 6 1 1
pyridoxal 5'-phosphate biosynthesis I 7 7 1
superpathway of purine nucleotide salvage 14 13 2
L-glutamate and L-glutamine biosynthesis 7 6 1
C4 photosynthetic carbon assimilation cycle, NADP-ME type 7 4 1
capsaicin biosynthesis 7 3 1
glyphosate degradation III 7 3 1
diacylglycerol and triacylglycerol biosynthesis 7 3 1
stigma estolide biosynthesis 7 2 1
ceramide degradation by α-oxidation 7 2 1
arachidonate biosynthesis III (6-desaturase, mammals) 7 1 1
icosapentaenoate biosynthesis II (6-desaturase, mammals) 7 1 1
icosapentaenoate biosynthesis III (8-desaturase, mammals) 7 1 1
partial TCA cycle (obligate autotrophs) 8 8 1
nitrogen remobilization from senescing leaves 8 6 1
superpathway of methylglyoxal degradation 8 5 1
superpathway of polyamine biosynthesis I 8 5 1
2-deoxy-D-ribose degradation II 8 4 1
(aminomethyl)phosphonate degradation 8 3 1
glutathione-mediated detoxification I 8 3 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 2
anandamide biosynthesis II 8 2 1
sesamin biosynthesis 8 1 1
superpathway of CDP-glucose-derived O-antigen building blocks biosynthesis 8 1 1
folate transformations III (E. coli) 9 9 1
superpathway of sulfate assimilation and cysteine biosynthesis 9 9 1
Entner-Doudoroff pathway I 9 8 1
sucrose biosynthesis I (from photosynthesis) 9 7 1
TCA cycle VI (Helicobacter) 9 7 1
reductive glycine pathway of autotrophic CO2 fixation 9 5 1
allantoin degradation IV (anaerobic) 9 2 1
gliotoxin biosynthesis 9 2 1
cis-geranyl-CoA degradation 9 2 1
glutathione-mediated detoxification II 9 1 1
L-arginine biosynthesis II (acetyl cycle) 10 10 1
superpathway of coenzyme A biosynthesis II (plants) 10 9 1
superpathway of tetrahydrofolate biosynthesis 10 8 1
glycolysis IV 10 8 1
superpathway of pyrimidine ribonucleosides salvage 10 7 1
3-phenylpropanoate degradation 10 4 1
suberin monomers biosynthesis 20 4 2
matairesinol biosynthesis 10 1 1
justicidin B biosynthesis 10 1 1
superpathway of fatty acid biosynthesis II (plant) 43 38 4
folate transformations II (plants) 11 10 1
glycolysis II (from fructose 6-phosphate) 11 9 1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 9 1
glycolysis III (from glucose) 11 9 1
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 7 1
glycolysis VI (from fructose) 11 7 1
superpathway of pyridoxal 5'-phosphate biosynthesis and salvage 12 11 1
formaldehyde assimilation III (dihydroxyacetone cycle) 12 10 1
superpathway of tetrahydrofolate biosynthesis and salvage 12 10 1
gluconeogenesis III 12 9 1
homolactic fermentation 12 9 1
superpathway of C1 compounds oxidation to CO2 12 5 1
indole glucosinolate activation (intact plant cell) 12 3 1
anandamide biosynthesis I 12 3 1
camalexin biosynthesis 12 2 1
superpathway of L-arginine and L-ornithine degradation 13 11 1
gluconeogenesis I 13 11 1
glycolysis I (from glucose 6-phosphate) 13 10 1
superpathway of cardiolipin biosynthesis (bacteria) 13 9 1
formaldehyde assimilation I (serine pathway) 13 7 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 8 1
superpathway of phospholipid biosynthesis II (plants) 28 10 2
Bifidobacterium shunt 15 12 1
palmitate biosynthesis II (type II fatty acid synthase) 31 29 2
mixed acid fermentation 16 12 1
glycerol degradation to butanol 16 9 1
cutin biosynthesis 16 1 1
plasmalogen biosynthesis I (aerobic) 16 1 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 14 1
superpathway of arginine and polyamine biosynthesis 17 14 1
superpathway of glucose and xylose degradation 17 14 1
superpathway of hexitol degradation (bacteria) 18 13 1
heterolactic fermentation 18 12 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 9 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 14 1
superpathway of anaerobic sucrose degradation 19 13 1
superpathway of seleno-compound metabolism 19 8 1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle 22 18 1
superpathway of N-acetylneuraminate degradation 22 12 1
ethene biosynthesis V (engineered) 25 18 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 22 1
superpathway of fatty acids biosynthesis (E. coli) 53 51 2
palmitate biosynthesis III 29 28 1
oleate β-oxidation 35 30 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 21 1
superpathway of chorismate metabolism 59 42 1