Experiment set7IT044 for Pseudomonas putida KT2440
5-Aminovaleric acid 10 mM carbon source
Group: carbon sourceMedia: MOPS minimal media_noCarbon + 5-Aminovaleric acid (10 mM)
Culturing: Putida_ML5_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 6.6 generations
By: Mitchell Thompson on 4/25/18
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 6 genes in this experiment
For carbon source 5-Aminovaleric acid in Pseudomonas putida KT2440
For carbon source 5-Aminovaleric acid across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Cobalt-zinc-cadmium resistance | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fatty acid metabolism
- Lysine degradation
- Tryptophan metabolism
- Glyoxylate and dicarboxylate metabolism
- Benzoate degradation via CoA ligation
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glutarate degradation | 2 | 2 | 2 |
| L-lysine degradation IV | 5 | 5 | 1 |
| L-lysine degradation X | 6 | 5 | 1 |
| L-lysine degradation I | 7 | 5 | 1 |
| photorespiration I | 9 | 6 | 1 |
| superpathway of L-lysine degradation | 43 | 23 | 2 |