Experiment set7IT035 for Pseudomonas simiae WCS417
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x
Group: supernatantMedia: RCH2_defined_noCarbon (0.9x) + supernatant from Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted (0.1 x)
Culturing: fluoroDangl_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 5/2/18
Media components: 0.225 g/L Ammonium chloride, 0.09 g/L Potassium Chloride, 0.54 g/L Sodium phosphate monobasic monohydrate, 27 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.027 g/L Magnesium Sulfate Heptahydrate, 0.0135 g/L Nitrilotriacetic acid, 0.009 g/L Sodium Chloride, 0.0045 g/L Manganese (II) sulfate monohydrate, 0.0009 g/L Cobalt chloride hexahydrate, 0.0009 g/L Zinc sulfate heptahydrate, 0.0009 g/L Calcium chloride dihydrate, 0.0009 g/L Iron (II) sulfate heptahydrate, 0.000225 g/L Nickel (II) chloride hexahydrate, 0.00018 g/L Aluminum potassium sulfate dodecahydrate, 9e-05 g/L Copper (II) sulfate pentahydrate, 9e-05 g/L Boric Acid, 9e-05 g/L Sodium Molybdate Dihydrate, 0.0027 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.09 mg/L Pyridoxine HCl, 0.045 mg/L 4-Aminobenzoic acid, 0.045 mg/L Lipoic acid, 0.045 mg/L Nicotinic Acid, 0.045 mg/L Riboflavin, 0.045 mg/L Thiamine HCl, 0.045 mg/L calcium pantothenate, 0.018 mg/L biotin, 0.018 mg/L Folic Acid, 0.0009 mg/L Cyanocobalamin) (final concentrations)
Growth plate: Plate3b B5
Specific Phenotypes
None in this experiment