Experiment set7IT029 for Pseudomonas simiae WCS417
RCH2_defined_glucose with MS_media 1x
Group: carbon sourceMedia: RCH2_defined_glucose + 1x MS_media
Culturing: fluoroDangl_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 5/2/18
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
MS_media 1x includes: 1650 mg/L Ammonium Nitrate, 6.2 mg/L Boric Acid, 332.2 mg/L Calcium chloride, 0.025 mg/L Cobalt chloride hexahydrate, 0.025 mg/L Copper (II) sulfate pentahydrate, 37.26 mg/L EDTA (disodium salt), 27.8 mg/L Iron (II) sulfate heptahydrate, 2 mg/L Glycine, 180.7 mg/L Magnesium sulfate, 16.9 mg/L Manganese sulfate, 100 mg/L m-Inositol, 0.5 mg/L Nicotinic Acid, 0.83 mg/L Potassium iodide, 1900 mg/L Potassium nitrate, 170 mg/L Potassium phosphate monobasic, 0.5 mg/L Pyridoxine HCl, 0.25 mg/L Sodium Molybdate Dihydrate, 0.1 mg/L Thiamine HCl, 8.6 mg/L Zinc sulfate heptahydrate, 20 g/L Sucrose
Growth plate: Plate3b A5
Specific Phenotypes
None in this experiment