Experiment set7IT010 for Pseudomonas simiae WCS417

Compare to:

RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x

Group: supernatant
Media: RCH2_defined_noCarbon (0.75x) + supernatant from Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted (0.25 x)
Culturing: fluoroDangl_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 5/2/18
Media components: 0.1875 g/L Ammonium chloride, 0.075 g/L Potassium Chloride, 0.45 g/L Sodium phosphate monobasic monohydrate, 22.5 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.0225 g/L Magnesium Sulfate Heptahydrate, 0.01125 g/L Nitrilotriacetic acid, 0.0075 g/L Sodium Chloride, 0.00375 g/L Manganese (II) sulfate monohydrate, 0.00075 g/L Cobalt chloride hexahydrate, 0.00075 g/L Zinc sulfate heptahydrate, 0.00075 g/L Calcium chloride dihydrate, 0.00075 g/L Iron (II) sulfate heptahydrate, 0.0001875 g/L Nickel (II) chloride hexahydrate, 0.00015 g/L Aluminum potassium sulfate dodecahydrate, 7.5e-05 g/L Copper (II) sulfate pentahydrate, 7.5e-05 g/L Boric Acid, 7.5e-05 g/L Sodium Molybdate Dihydrate, 0.00225 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.075 mg/L Pyridoxine HCl, 0.0375 mg/L 4-Aminobenzoic acid, 0.0375 mg/L Lipoic acid, 0.0375 mg/L Nicotinic Acid, 0.0375 mg/L Riboflavin, 0.0375 mg/L Thiamine HCl, 0.0375 mg/L calcium pantothenate, 0.015 mg/L biotin, 0.015 mg/L Folic Acid, 0.00075 mg/L Cyanocobalamin) (final concentrations)
Growth plate: Plate3a B4

Specific Phenotypes

None in this experiment

For Pseudomonas simiae WCS417 in supernatant experiments

For supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted across organisms