Experiment set78S380 for Escherichia coli BW25113
CTR
Group: no phage controlMedia: LB_plus_SM_buffer + double orbital, continuous, 205cpm (10 MOI)
Culturing: Keio_ML9a, 48 well microplate, Liquid, at 37 (C), shaken=Aerobic, (No phage control)
By: Sarshad on 10/6/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 3 genes in this experiment
For no phage control double orbital, continuous, 205cpm in Escherichia coli BW25113
For no phage control double orbital, continuous, 205cpm across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Ribosome LSU bacterial | 1 |
| Selenocysteine metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-selenocysteine biosynthesis I (bacteria) | 3 | 3 | 1 |
| L-selenocysteine biosynthesis II (archaea and eukaryotes) | 4 | 2 | 1 |