Experiment set75S661 for Escherichia coli BW25113

NpK

Group: phage
Media: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: Keio_ML9a, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 8/11/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 9 genes in this experiment

For phage Phage in Escherichia coli BW25113

For phage Phage across organisms

SEED Subsystems

Subsystem	#Specific
Iron acquisition in Vibrio	3
Transport of Iron	3
Campylobacter Iron Metabolism	1
CytR regulation	1
Deoxyribose and Deoxynucleoside Catabolism	1
Oxidative stress	1
Siderophore Aerobactin	1
Transport of Zinc	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
2-deoxy-α-D-ribose 1-phosphate degradation	3	3	1
2-deoxy-D-ribose degradation I	3	3	1
superpathway of pyrimidine deoxyribonucleosides degradation	6	6	1
superpathway of purine deoxyribonucleosides degradation	7	7	1
peptidoglycan recycling II	10	7	1
peptidoglycan recycling I	14	14	1