Experiment set74S187 for Escherichia coli BW25113
Goslar
Group: phageMedia: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: KEIO_ML9a, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 6/6/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 15 genes in this experiment
For phage Phage in Escherichia coli BW25113
For phage Phage across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| LOS core oligosaccharide biosynthesis | 1 |
| Orphan regulatory proteins | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Lipopolysaccharide biosynthesis
- Pentose phosphate pathway
- Glycine, serine and threonine metabolism
- Glycerolipid metabolism
- Glyoxylate and dicarboxylate metabolism
- Nicotinate and nicotinamide metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| 2-O-α-mannosyl-D-glycerate degradation | 2 | 2 | 1 |
| D-glucarate degradation I | 4 | 4 | 1 |
| D-galactarate degradation I | 4 | 4 | 1 |
| glycolate and glyoxylate degradation I | 4 | 4 | 1 |
| superpathway of D-glucarate and D-galactarate degradation | 5 | 5 | 1 |
| lipid A-core biosynthesis (E. coli K-12) | 10 | 9 | 2 |
| superpathway of glycol metabolism and degradation | 7 | 7 | 1 |
| 2-deoxy-D-ribose degradation II | 8 | 3 | 1 |
| Entner-Doudoroff pathway II (non-phosphorylative) | 9 | 6 | 1 |
| photorespiration III | 9 | 5 | 1 |
| photorespiration I | 9 | 5 | 1 |
| lipid A-core biosynthesis (Salmonella) | 10 | 8 | 1 |
| photorespiration II | 10 | 6 | 1 |
| superpathway of Kdo2-lipid A biosynthesis | 25 | 24 | 2 |
| formaldehyde assimilation I (serine pathway) | 13 | 8 | 1 |
| superpathway of microbial D-galacturonate and D-glucuronate degradation | 31 | 19 | 1 |