Experiment set74S168 for Escherichia coli BW25113

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Control_KEIO

200 most important genes:

  gene name fitness t score description  
b3977 tyrU -4.4 -1.5 tRNA-Tyr (NCBI) compare
b2620 smpB -3.5 -2.4 SsrA-binding protein (NCBI) compare
b2168 fruK -3.5 -2.0 1-phosphofructokinase (NCBI) compare
b3390 aroK -3.3 -3.0 shikimate kinase I (NCBI) compare
b0683 fur -3.0 -1.7 ferric uptake regulator (NCBI) compare
b1493 gadB -2.7 -1.3 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b1860 ruvB -2.6 -1.8 Holliday junction DNA helicase B (NCBI) compare
b3169 nusA -2.6 -4.4 transcription elongation factor NusA (NCBI) compare
b3183 obgE -2.6 -1.7 GTPase involved in cell partioning and DNA repair (NCBI) compare
b0053 surA -2.6 -5.5 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b0564 appY -2.4 -1.5 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b3863 polA -2.3 -1.6 DNA polymerase I (NCBI) compare
b1161 ycgX -2.2 -1.1 hypothetical protein (NCBI) compare
b2699 recA -2.2 -1.5 recombinase A (NCBI) compare
b0200 gmhB -2.1 -1.5 hypothetical protein (NCBI) compare
b3619 rfaD -2.1 -4.6 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3613 yibP -2.1 -3.9 putative membrane protein (VIMSS) compare
b2846 yqeH -2.1 -1.2 orf, hypothetical protein (VIMSS) compare
b1765 ydjA -2.0 -1.4 predicted oxidoreductase (NCBI) compare
b0439 lon -2.0 -5.9 DNA-binding ATP-dependent protease La (NCBI) compare
b0630 lipB -1.9 -1.3 protein of lipoate biosynthesis (VIMSS) compare
b2282 nuoH -1.9 -3.8 NADH dehydrogenase subunit H (NCBI) compare
b4042 dgkA -1.8 -2.8 diacylglycerol kinase (NCBI) compare
b4451 ryhB -1.8 -1.5 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b2894 xerD -1.8 -1.2 site-specific tyrosine recombinase XerD (NCBI) compare
b1129 phoQ -1.7 -1.7 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b1984 asnW -1.7 -1.3 tRNA-Asn (NCBI) compare
b1290 sapF -1.7 -2.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0049 apaH -1.7 -3.5 diadenosinetetraphosphatase (NCBI) compare
b3979 thrT -1.6 -0.5 tRNA-Thr (NCBI) compare
b2287 nuoB -1.6 -3.0 NADH dehydrogenase subunit B (NCBI) compare
b3811 xerC -1.6 -1.1 site-specific tyrosine recombinase XerC (NCBI) compare
b3052 rfaE -1.6 -4.4 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3778 rep -1.6 -5.9 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b2281 nuoI -1.5 -2.9 NADH dehydrogenase subunit I (NCBI) compare
b2528 iscA -1.5 -1.6 iron-sulfur cluster assembly protein (NCBI) compare
b2820 recB -1.5 -1.0 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b3624 rfaZ -1.5 -2.4 lipopolysaccharide core biosynthesis protein (NCBI) compare
b3816 corA -1.5 -6.0 magnesium/nickel/cobalt transporter (NCBI) compare
b2830 nudH -1.4 -1.4 dinucleoside polyphosphate hydrolase (NCBI) compare
b2279 nuoK -1.4 -2.4 NADH dehydrogenase subunit K (NCBI) compare
b1236 galU -1.4 -3.9 glucose-1-phosphate uridylyltransferase (NCBI) compare
b1842 holE -1.4 -2.0 DNA polymerase III, theta subunit (NCBI) compare
b3706 trmE -1.4 -4.8 tRNA modification GTPase (NCBI) compare
b2286 nuoC -1.4 -3.9 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b3026 qseC -1.4 -3.9 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b1850 eda -1.4 -1.3 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b1292 sapC -1.4 -2.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0088 murD -1.3 -2.9 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b2935 tktA -1.3 -2.2 transketolase 1, thiamin-binding (NCBI) compare
b3839 tatC -1.3 -3.9 TatABCE protein translocation system subunit (NCBI) compare
b3290 trkA -1.3 -2.0 potassium transporter peripheral membrane component (NCBI) compare
b2288 nuoA -1.3 -3.2 NADH dehydrogenase I chain A (VIMSS) compare
b3426 glpD -1.3 -3.3 sn-glycerol-3-phosphate dehydrogenase, aerobic, FAD/NAD(P)-binding (NCBI) compare
b1613 manA -1.3 -2.0 mannose-6-phosphate isomerase (VIMSS) compare
b3620 rfaF -1.3 -4.4 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b2277 nuoM -1.3 -3.9 NADH dehydrogenase subunit M (NCBI) compare
b4171 miaA -1.2 -3.1 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b4201 priB -1.2 -2.5 primosomal replication protein N (NCBI) compare
b3792 wzxE -1.2 -5.4 O-antigen translocase (NCBI) compare
b3728 pstS -1.2 -2.6 phosphate transporter subunit (NCBI) compare
b0738 tolR -1.2 -2.5 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0437 clpP -1.2 -3.2 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b1863 ruvC -1.2 -1.6 Holliday junction resolvase (NCBI) compare
b2278 nuoL -1.2 -6.0 NADH dehydrogenase subunit L (NCBI) compare
b1160 elbA -1.2 -1.3 hypothetical protein (NCBI) compare
b2897 ygfY -1.1 -3.0 hypothetical protein (NCBI) compare
b0080 fruR -1.1 -3.9 DNA-binding transcriptional dual regulator (NCBI) compare
b3405 ompR -1.1 -3.1 osmolarity response regulator (NCBI) compare
b3741 gidA -1.1 -3.2 glucose-inhibited division protein A (NCBI) compare
b2318 truA -1.1 -2.1 tRNA pseudouridine synthase A (NCBI) compare
b0740 tolB -1.1 -3.2 translocation protein TolB precursor (NCBI) compare
b3021 ygiT -1.1 -1.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b0051 ksgA -1.1 -2.6 dimethyladenosine transferase (NCBI) compare
b2276 nuoN -1.1 -3.5 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b3726 pstA -1.1 -3.6 phosphate transporter subunit (NCBI) compare
b1291 sapD -1.1 -1.2 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4698 mgrR -1.1 -2.2 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b1476 fdnI -1.1 -1.0 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b2330 yfcB -1.1 -1.4 putative adenine-specific methylase (VIMSS) compare
b4647 mokA -1.0 -0.8 no description compare
b4402 yjjY -1.0 -0.9 hypothetical protein (NCBI) compare
b1347 ydaC -1.0 -0.5 Rac prophage; predicted protein (NCBI) compare
b2283 nuoG -1.0 -4.6 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b1855 lpxM -1.0 -2.5 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b4515 ybgT -1.0 -1.5 hypothetical protein (NCBI) compare
b1306 pspC -1.0 -1.9 DNA-binding transcriptional activator (NCBI) compare
b1804 rnd -1.0 -3.5 ribonuclease D (NCBI) compare
b4146 yjeK -1.0 -1.6 predicted lysine aminomutase (NCBI) compare
b1165 ymgA -1.0 -0.6 hypothetical protein (NCBI) compare
b0888 trxB -1.0 -2.6 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b1951 rcsA -1.0 -1.8 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b4702 mgtL -1.0 -1.7 regulatory leader peptide for mgtA (NCBI) compare
b1351 racC -1.0 -0.6 Rac prophage; predicted protein (NCBI) compare
b0890 ftsK -0.9 -2.8 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b0737 tolQ -0.9 -2.0 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b4685 yrbN -0.9 -1.8 expressed protein (RefSeq) compare
b2280 nuoJ -0.9 -2.8 NADH dehydrogenase subunit J (NCBI) compare
b1594 dgsA -0.9 -2.5 DNA-binding transcriptional repressor (NCBI) compare
b4202 rpsR -0.9 -1.1 30S ribosomal protein S18 (NCBI) compare
b3636 rpmG -0.9 -0.9 50S ribosomal protein L33 (NCBI) compare
b4585 sroB -0.9 -1.2 Novel sRNA, function unknown (RefSeq) compare
b3631 rfaG -0.9 -6.1 glucosyltransferase I (NCBI) compare
b3916 pfkA -0.9 -2.2 6-phosphofructokinase (NCBI) compare
b0677 nagA -0.9 -3.7 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b4653 yicT -0.8 -1.5 no description compare
b0741 pal -0.8 -1.8 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b4679 yohP -0.8 -1.8 expressed protein (RefSeq) compare
b1175 minD -0.8 -2.7 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b4177 purA -0.8 -0.8 adenylosuccinate synthetase (NCBI) compare
b1084 rne -0.8 -3.5 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b3181 greA -0.8 -3.2 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b2284 nuoF -0.8 -3.0 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b1628 rsxB -0.8 -1.4 electron transport complex protein RnfB (NCBI) compare
b4613 dinQ -0.8 -1.2 Damage inducible, function unknown (RefSeq) compare
b3649 rpoZ -0.8 -1.9 DNA-directed RNA polymerase subunit omega (NCBI) compare
b3630 rfaP -0.8 -4.7 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b2947 gshB -0.8 -2.2 glutathione synthetase (NCBI) compare
b1108 ycfP -0.8 -2.0 orf, hypothetical protein (VIMSS) compare
b0767 ybhE -0.8 -1.5 6-phosphogluconolactonase (NCBI) compare
b1299 puuR -0.8 -2.4 DNA-binding transcriptional repressor (NCBI) compare
b4631 ykgQ -0.8 -1.3 no description compare
b3781 trxA -0.8 -1.9 thioredoxin 1 (VIMSS) compare
b2962 yggX -0.8 -2.1 hypothetical protein (NCBI) compare
b1286 rnb -0.8 -1.4 exoribonuclease II (NCBI) compare
b3807 cyaY -0.8 -3.1 frataxin-like protein (NCBI) compare
b2906 visC -0.8 -3.3 hypothetical protein (NCBI) compare
b2652 ileY -0.8 -0.5 tRNA-Ile (NCBI) compare
b3438 gntR -0.8 -3.7 DNA-binding transcriptional repressor (NCBI) compare
b1130 phoP -0.8 -1.4 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b0471 ybaB -0.7 -1.5 hypothetical protein (NCBI) compare
b1380 ldhA -0.7 -2.0 D-lactate dehydrogenase (NCBI) compare
b1712 ihfA -0.7 -1.0 integration host factor subunit alpha (NCBI) compare
b2024 hisA -0.7 -1.8 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b4345 mcrC -0.7 -0.7 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b4513 kdpF -0.7 -2.0 potassium ion accessory transporter subunit (NCBI) compare
b3775 ppiC -0.7 -2.2 peptidyl-prolyl cis-trans isomerase C (rotamase C) (NCBI) compare
b2498 upp -0.7 -1.3 uracil phosphoribosyltransferase (VIMSS) compare
b1658 purR -0.7 -3.0 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b0232 yafN -0.7 -0.6 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b4012 yjaB -0.7 -2.1 predicted acetyltransferase (NCBI) compare
b0623 cspE -0.7 -1.3 cold shock protein E (NCBI) compare
b0113 pdhR -0.7 -3.1 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b0912 ihfB -0.7 -1.6 integration host factor subunit beta (NCBI) compare
b0849 grxA -0.7 -2.4 glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a) (NCBI) compare
b3162 deaD -0.7 -2.2 inducible ATP-independent RNA helicase (VIMSS) compare
b3621 rfaC -0.7 -2.8 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3398 yrfF -0.7 -2.6 predicted inner membrane protein (NCBI) compare
b0438 clpX -0.7 -3.6 ATP-dependent protease ATP-binding subunit (NCBI) compare
b0726 sucA -0.7 -1.7 alpha-ketoglutarate decarboxylase (NCBI) compare
b1040 csgD -0.7 -1.0 DNA-binding transcriptional activator in two-component regulatory system (NCBI) compare
b1518 lsrG -0.7 -0.9 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b3844 fre -0.7 -2.4 NAD(P)H-flavin reductase (NCBI) compare
b1334 fnr -0.7 -1.5 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b0407 yajC -0.7 -1.5 preprotein translocase subunit YajC (NCBI) compare
b0685 ybfE -0.7 -0.9 orf, hypothetical protein (VIMSS) compare
b1620 malI -0.7 -0.9 DNA-binding transcriptional repressor (NCBI) compare
b0977 hyaF -0.7 -2.0 protein involved in nickel incorporation into hydrogenase-1 proteins (NCBI) compare
b1949 fliQ -0.6 -2.0 flagellar biosynthesis protein Q (NCBI) compare
b3261 fis -0.6 -0.9 DNA-binding protein Fis (NCBI) compare
b2768 ygcP -0.6 -3.0 predicted anti-terminator regulatory protein (NCBI) compare
b3860 dsbA -0.6 -3.3 periplasmic protein disulfide isomerase I (NCBI) compare
b0721 sdhC -0.6 -2.5 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b3389 aroB -0.6 -3.4 3-dehydroquinate synthase (NCBI) compare
b0728 sucC -0.6 -2.4 succinyl-CoA synthetase subunit beta (NCBI) compare
b0791 ybhQ -0.6 -1.8 predicted inner membrane protein (NCBI) compare
b1729 ydjN -0.6 -1.3 predicted transporter (NCBI) compare
b2476 purC -0.6 -1.3 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b1088 yceD -0.6 -1.5 hypothetical protein (NCBI) compare
b1086 rluC -0.6 -2.6 23S rRNA pseudouridylate synthase (NCBI) compare
b0386 proC -0.6 -1.7 pyrroline-5-carboxylate reductase (NCBI) compare
b0004 thrC -0.6 -3.3 threonine synthase (NCBI) compare
b2707 srlR -0.6 -2.6 DNA-bindng transcriptional repressor (NCBI) compare
b1279 yciS -0.6 -0.8 conserved inner membrane protein (NCBI) compare
b1630 rsxD -0.6 -2.8 electron transport complex protein RnfD (NCBI) compare
b1939 fliG -0.6 -1.3 flagellar motor switch protein G (NCBI) compare
b1563 relE -0.6 -0.7 Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI) compare
b3867 hemN -0.6 -3.9 O2-independent coproporphyrinogen III oxidase (VIMSS) compare
b2340 sixA -0.6 -2.5 phosphohistidine phosphatase (NCBI) compare
b1400 paaY -0.6 -2.2 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b0605 ahpC -0.6 -1.6 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b0556 rzpD -0.6 -1.7 DLP12 prophage; predicted murein endopeptidase (NCBI) compare
b2674 nrdI -0.6 -1.7 hypothetical protein (NCBI) compare
b1777 b1777 -0.6 -1.0 orf, hypothetical protein (VIMSS) compare
b4481 rffT -0.6 -2.7 4-alpha-L-fucosyltransferase (NCBI) compare
b2822 recC -0.6 -2.9 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b4025 pgi -0.6 -3.2 glucose-6-phosphate isomerase (NCBI) compare
b4441 glmY -0.6 -1.0 sRNA activator of glmS mRNA (RefSeq) compare
b4179 vacB -0.6 -2.5 putative enzyme (VIMSS) compare
b2553 glnB -0.6 -2.4 regulatory protein P-II for glutamine synthetase (NCBI) compare
b4232 fbp -0.6 -3.7 fructose-1,6-bisphosphatase (NCBI) compare
b3414 gntY -0.6 -2.1 predicted gluconate transport associated protein (NCBI) compare
b2285 nuoE -0.6 -1.1 NADH dehydrogenase subunit E (NCBI) compare
b2808 gcvA -0.6 -1.7 DNA-binding transcriptional dual regulator (NCBI) compare
b2325 yfcL -0.6 -1.1 hypothetical protein (NCBI) compare
b3292 zntR -0.6 -1.7 zinc-responsive transcriptional regulator (NCBI) compare
b0251 yafY -0.6 -1.8 CP4-6 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b1969 yedW -0.6 -2.6 putative 2-component transcriptional regulator (VIMSS) compare
b3138 agaB -0.6 -1.9 N-acetylgalactosamine-specific enzyme IIB component of PTS (NCBI) compare
b1125 potB -0.6 -1.7 spermidine/putrescine ABC transporter membrane protein (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in no phage control experiments

For no phage control No phage across organisms