Experiment set74S125 for Escherichia coli BW25113
Mnm2
Group: phageMedia: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: KEIO_ML9a, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 6/6/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 12 genes in this experiment
For phage Phage in Escherichia coli BW25113
For phage Phage across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Siderophore Enterobactin | 3 |
| Transport of Iron | 3 |
| Iron acquisition in Vibrio | 2 |
| Campylobacter Iron Metabolism | 1 |
| Ton and Tol transport systems | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Biosynthesis of siderophore group nonribosomal peptides
- Lipopolysaccharide biosynthesis
- Fatty acid biosynthesis
- Lysine degradation
- Histidine metabolism
- Tyrosine metabolism
- Phenylalanine metabolism
- Nucleotide sugars metabolism
- Glycerophospholipid metabolism
- Ether lipid metabolism
- Glycosphingolipid biosynthesis - ganglio series
- 1- and 2-Methylnaphthalene degradation
- Benzoate degradation via CoA ligation
- Ethylbenzene degradation
- Butanoate metabolism
- Porphyrin and chlorophyll metabolism
- Limonene and pinene degradation
- Diterpenoid biosynthesis
- Carotenoid biosynthesis - General
- Anthocyanin biosynthesis
- Alkaloid biosynthesis I
- Alkaloid biosynthesis II
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of type II polyketide backbone
- Biosynthesis of phenylpropanoids
- Biosynthesis of terpenoids and steroids
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| 2,3-dihydroxybenzoate biosynthesis | 3 | 3 | 1 |
| vibriobactin biosynthesis | 9 | 4 | 2 |
| enterobactin biosynthesis | 11 | 7 | 2 |
| bacillibactin biosynthesis | 12 | 6 | 2 |
| vanchrobactin biosynthesis | 8 | 3 | 1 |
| anguibactin biosynthesis | 8 | 2 | 1 |
| acinetobactin biosynthesis | 9 | 3 | 1 |
| myxochelin A and B biosynthesis | 9 | 2 | 1 |
| superpathway of chorismate metabolism | 59 | 54 | 2 |