Experiment set74S110 for Escherichia coli BW25113

TP12

Group: phage
Media: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: KEIO_ML9a, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 6/6/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 12 genes in this experiment

For phage Phage in Escherichia coli BW25113

For phage Phage across organisms

SEED Subsystems

Subsystem	#Specific
Siderophore Enterobactin	3
Transport of Iron	3
Glycerol and Glycerol-3-phosphate Uptake and Utilization	2
Iron acquisition in Vibrio	2
Biogenesis of cytochrome c oxidases	1
Campylobacter Iron Metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Biosynthesis of siderophore group nonribosomal peptides
• Lipopolysaccharide biosynthesis
• Fatty acid biosynthesis
• Lysine degradation
• Histidine metabolism
• Tyrosine metabolism
• Phenylalanine metabolism
• Nucleotide sugars metabolism
• Glycerophospholipid metabolism
• Ether lipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Ethylbenzene degradation
• Butanoate metabolism
• Porphyrin and chlorophyll metabolism
• Limonene and pinene degradation
• Diterpenoid biosynthesis
• Carotenoid biosynthesis - General
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide backbone
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
thiosulfate disproportionation IV (rhodanese)	1	1	1
2,3-dihydroxybenzoate biosynthesis	3	3	1
vibriobactin biosynthesis	9	4	2
sulfide oxidation IV (mitochondria)	5	2	1
enterobactin biosynthesis	11	7	2
bacillibactin biosynthesis	12	6	2
vanchrobactin biosynthesis	8	3	1
anguibactin biosynthesis	8	2	1
acinetobactin biosynthesis	9	3	1
myxochelin A and B biosynthesis	9	2	1
superpathway of chorismate metabolism	59	54	2