Experiment set6S81 for Pseudomonas sp. RS175

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p-Coumaric acid carbon source

Group: carbon source
Media: MME_noCarbon + p-Coumaric acid (2 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C)
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 2 genes in this experiment

For carbon source p-Coumaric acid in Pseudomonas sp. RS175

For carbon source p-Coumaric acid across organisms

SEED Subsystems

Subsystem #Specific
p-Hydroxybenzoate degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
4-chlorobenzoate degradation 4 2 1
4-methylphenol degradation to protocatechuate 4 1 1
4-coumarate degradation (aerobic) 5 2 1
bisphenol A degradation 5 1 1
4-hydroxymandelate degradation 6 2 1
spongiadioxin C biosynthesis 7 2 1
polybrominated dihydroxylated diphenyl ethers biosynthesis 8 2 1
toluene degradation III (aerobic) (via p-cresol) 11 7 1
superpathway of aerobic toluene degradation 30 11 1
superpathway of aromatic compound degradation via 3-oxoadipate 35 13 1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate 42 10 1