Experiment set6S57 for Escherichia coli ECOR27
CPL00187
Group: phageMedia: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: Ecoli_ECOR27_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 12/9/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 19 genes in this experiment
For phage Phage in Escherichia coli ECOR27
For phage Phage across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Fructose utilization | 1 |
| Queuosine-Archaeosine Biosynthesis | 1 |
| Type IV pilus | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Carotenoid biosynthesis - General
- Glycolysis / Gluconeogenesis
- Pentose and glucuronate interconversions
- Fructose and mannose metabolism
- Galactose metabolism
- Ascorbate and aldarate metabolism
- Biosynthesis of steroids
- Ubiquinone and menaquinone biosynthesis
- Histidine metabolism
- Tyrosine metabolism
- Tryptophan metabolism
- Benzoxazinone biosynthesis
- Starch and sucrose metabolism
- Nucleotide sugars metabolism
- Aminosugars metabolism
- Lipopolysaccharide biosynthesis
- Peptidoglycan biosynthesis
- Naphthalene and anthracene degradation
- Porphyrin and chlorophyll metabolism
- Phenylpropanoid biosynthesis
- Flavonoid biosynthesis
- Anthocyanin biosynthesis
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
- Biosynthesis of alkaloids derived from shikimate pathway
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| di-trans,poly-cis-undecaprenyl phosphate biosynthesis | 2 | 2 | 1 |
| queuosine biosynthesis I (de novo) | 4 | 4 | 1 |
| polyisoprenoid biosynthesis (E. coli) | 5 | 5 | 1 |
| queuosine biosynthesis III (queuosine salvage) | 5 | 3 | 1 |
| juvenile hormone III biosynthesis I | 6 | 1 | 1 |