Experiment set6S56 for Pseudomonas sp. RS175

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Betaine hydrochloride carbon source

Group: carbon source
Media: MME_noCarbon + Betaine hydrochloride (10 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C)
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 6 genes in this experiment

For carbon source Betaine hydrochloride in Pseudomonas sp. RS175

For carbon source Betaine hydrochloride across organisms

SEED Subsystems

Subsystem #Specific
Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. 1
Folate Biosynthesis 1
Molybdenum cofactor biosynthesis 1
Phosphate metabolism 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
bis(guanylyl molybdenum cofactor) biosynthesis 2 2 2
L-glutamine degradation I 1 1 1
bis(guanylyl tungstenpterin) cofactor biosynthesis 1 1 1
guanylyl molybdenum cofactor biosynthesis 1 1 1
L-glutamate biosynthesis I 2 2 1
4-aminobenzoate biosynthesis I 2 2 1
ammonia assimilation cycle III 3 3 1
glutaminyl-tRNAgln biosynthesis via transamidation 4 4 1
L-asparagine biosynthesis III (tRNA-dependent) 4 4 1
NAD(P)/NADPH interconversion 6 3 1
L-glutamate and L-glutamine biosynthesis 7 5 1
L-citrulline biosynthesis 8 7 1
chloramphenicol biosynthesis 9 1 1
superpathway of tetrahydrofolate biosynthesis 10 8 1
superpathway of candicidin biosynthesis 11 4 1
superpathway of tetrahydrofolate biosynthesis and salvage 12 10 1
superpathway of L-citrulline metabolism 12 9 1
superpathway of chorismate metabolism 59 43 1