Experiment set6S53 for Pseudomonas sp. RS175

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4-Guanidinobutyric acid carbon source

Group: carbon source
Media: MME_noCarbon + 4-Guanidinobutyric acid (10 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C)
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 5 genes in this experiment

For carbon source 4-Guanidinobutyric acid in Pseudomonas sp. RS175

For carbon source 4-Guanidinobutyric acid across organisms

SEED Subsystems

Subsystem #Specific
Arginine and Ornithine Degradation 1
DNA-binding regulatory proteins, strays 1
Polyamine Metabolism 1
Purine Utilization 1
ZZ gjo need homes 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-arginine degradation III (arginine decarboxylase/agmatinase pathway) 2 2 1
putrescine biosynthesis I 2 2 1
L-arginine degradation X (arginine monooxygenase pathway) 3 2 1
superpathway of putrescine biosynthesis 4 3 1
L-arginine degradation IX (arginine:pyruvate transaminase pathway) 4 3 1
L-arginine degradation VIII (arginine oxidase pathway) 4 2 1
L-arginine degradation XII 4 1 1
superpathway of polyamine biosynthesis I 8 5 1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 7 1
superpathway of L-arginine and L-ornithine degradation 13 9 1
superpathway of arginine and polyamine biosynthesis 17 14 1