Experiment set6S40 for Pseudomonas sp. RS175

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Guanosine carbon source

Group: carbon source
Media: MME_noCarbon + Guanosine (10 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C)
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 7 genes in this experiment

For carbon source Guanosine in Pseudomonas sp. RS175

For carbon source Guanosine across organisms

SEED Subsystems

Subsystem #Specific
Purine Utilization 4
Allantoin Utilization 1
Photorespiration (oxidative C2 cycle) 1
Purine conversions 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
guanosine nucleotides degradation III 4 4 2
guanosine nucleotides degradation II 4 4 2
adenosine nucleotides degradation II 5 5 2
superpathway of guanosine nucleotides degradation (plants) 6 5 2
purine nucleotides degradation II (aerobic) 11 11 3
glycolate and glyoxylate degradation I 4 4 1
inosine 5'-phosphate degradation 4 4 1
adenosine nucleotides degradation I 8 7 2
purine nucleotides degradation I (plants) 12 10 3
guanosine nucleotides degradation I 4 3 1
L-threonate degradation 4 1 1
D-erythronate degradation II 4 1 1
purine nucleobases degradation II (anaerobic) 24 16 5
superpathway of purines degradation in plants 18 14 3
superpathway of glycol metabolism and degradation 7 6 1
ureide biosynthesis 7 6 1
drosopterin and aurodrosopterin biosynthesis 7 5 1
caffeine degradation III (bacteria, via demethylation) 7 1 1
theophylline degradation 9 1 1
caffeine degradation IV (bacteria, via demethylation and oxidation) 10 1 1
purine nucleobases degradation I (anaerobic) 15 6 1