Experiment set6S39 for Bosea sp. OAE506

R2A with 2-pentyl-furan 21.3684 mM

Group: stress
Media: R2A + 2-pentyl-furan (21.3684 mM)
Culturing: Bosea_OAE506_ML2, tube, Aerobic, at 30 (C), shaken=200 rpm
By: Cat Adams on 2/14/24
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate

Specific Phenotypes

For 28 genes in this experiment

For stress 2-pentyl-furan in Bosea sp. OAE506

For stress 2-pentyl-furan across organisms

SEED Subsystems

Subsystem	#Specific
Molybdenum cofactor biosynthesis	3
Transport of Molybdenum	2
Acetyl-CoA fermentation to Butyrate	1
Ammonia assimilation	1
Lipid A-Ara4N pathway ( Polymyxin resistance )	1
N-heterocyclic aromatic compound degradation	1
Peptidoglycan Biosynthesis	1
Polyhydroxybutyrate metabolism	1
Potassium homeostasis	1
Protein degradation	1
Serine-glyoxylate cycle	1
Teichuronic acid biosynthesis	1
Type IV pilus	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Peptidoglycan biosynthesis
• Fructose and mannose metabolism
• Ascorbate and aldarate metabolism
• Butanoate metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Pentose and glucuronate interconversions
• C21-Steroid hormone metabolism
• Puromycin biosynthesis
• Glycine, serine and threonine metabolism
• Lysine degradation
• Bisphenol A degradation
• Starch and sucrose metabolism
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Polyketide sugar unit biosynthesis
• Aminosugars metabolism
• Keratan sulfate biosynthesis
• Lipopolysaccharide biosynthesis
• Glycerolipid metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Linoleic acid metabolism
• alpha-Linolenic acid metabolism
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Tetrachloroethene degradation
• Benzoate degradation via CoA ligation
• Trinitrotoluene degradation
• Biotin metabolism
• Retinol metabolism
• Porphyrin and chlorophyll metabolism
• Zeatin biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Flavone and flavonol biosynthesis
• Aminoacyl-tRNA biosynthesis
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide products
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UDP-α-D-glucuronate biosynthesis (from UDP-glucose)	1	1	1
UDP-α-D-xylose biosynthesis	2	2	1
UDP-N-acetyl-α-D-galactosaminuronate biosynthesis	2	1	1
nitroglycerin degradation	5	2	2
polyhydroxybutanoate biosynthesis	3	2	1
molybdenum cofactor biosynthesis	3	2	1
(2S)-ethylmalonyl-CoA biosynthesis	4	3	1
bis(tungstenpterin) cofactor biosynthesis	4	1	1
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered)	5	3	1
UDP-2,3-diacetamido-2,3-dideoxy-α-D-mannuronate biosynthesis	5	1	1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis	6	5	1
acetyl-CoA fermentation to butanoate	7	5	1
peptidoglycan biosynthesis II (staphylococci)	17	12	2
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	2
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
UDP-sugars interconversion	9	4	1
teichuronic acid biosynthesis (B. subtilis 168)	9	2	1
4-oxopentanoate degradation	9	2	1
myxochelin A and B biosynthesis	9	1	1
colanic acid building blocks biosynthesis	11	9	1
ethylmalonyl-CoA pathway	11	6	1
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	11	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	3	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1
tRNA charging	21	19	1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis	24	6	1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered)	26	18	1