Experiment set6S29 for Pseudomonas sp. RS175

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Cytidine carbon source

Group: carbon source
Media: MME_noCarbon + Cytidine (10 mM)
Culturing: Pseudomonas_RS175_ML2, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C)
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 4 genes in this experiment

For carbon source Cytidine in Pseudomonas sp. RS175

For carbon source Cytidine across organisms

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
UDP-α-D-glucose biosynthesis 2 2 1
trehalose degradation V 3 2 1
GDP-α-D-glucose biosynthesis 3 2 1
starch degradation V 4 3 1
glycogen biosynthesis I (from ADP-D-Glucose) 4 3 1
sucrose degradation IV (sucrose phosphorylase) 4 3 1
starch degradation III 4 2 1
dTDP-β-L-rhamnose biosynthesis 5 5 1
sucrose degradation II (sucrose synthase) 5 4 1
glucose and glucose-1-phosphate degradation 5 4 1
D-galactose degradation I (Leloir pathway) 5 3 1
CDP-6-deoxy-D-gulose biosynthesis 5 3 1
glucosylglycerol biosynthesis 5 2 1
glycogen degradation II 6 5 1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis 6 4 1
glycogen degradation I 8 7 1
sucrose biosynthesis II 8 6 1
glycogen biosynthesis III (from α-maltose 1-phosphate) 8 3 1
sucrose biosynthesis I (from photosynthesis) 9 7 1
chitin biosynthesis 9 6 1
glycolysis IV 10 8 1
starch biosynthesis 10 5 1
O-antigen building blocks biosynthesis (E. coli) 11 10 1
colanic acid building blocks biosynthesis 11 9 1
streptomycin biosynthesis 18 3 1
superpathway of anaerobic sucrose degradation 19 15 1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis 19 6 1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle 22 17 1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 12 1