Experiment set6S259 for Escherichia coli ECRC101

GL_40

Group: toxin
Media: LB + Gliotoxin (40 ug/mL)
Culturing: Ecoli_ECRC101_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Phoebe on 5/17/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 17 genes in this experiment

For toxin Gliotoxin in Escherichia coli ECRC101

For toxin Gliotoxin across organisms

SEED Subsystems

Subsystem	#Specific
Multidrug Resistance Efflux Pumps	2
Transport of Zinc	2
Lipid A modifications	1
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	1
NAD and NADP cofactor biosynthesis global	1
NAD regulation	1
Orphan regulatory proteins	1
Oxidative stress	1
Periplasmic disulfide interchange	1
Redox-dependent regulation of nucleus processes	1
Transport of Manganese	1
Universal stress protein family	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nicotinate and nicotinamide metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
NAD salvage pathway V (PNC V cycle)	5	3	1
NAD salvage pathway I (PNC VI cycle)	7	5	1
NAD salvage (plants)	11	5	1
superpathway of NAD biosynthesis in eukaryotes	14	7	1