Experiment set6S187 for Escherichia coli BL21

Compare to:

Bas49

Group: phage
Media: LB_plus_SM_buffer + MOI 10
Culturing: BL21_ML4, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 9/20/23
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 37 genes in this experiment

For phage MOI 10 in Escherichia coli BL21

For phage MOI 10 across organisms

SEED Subsystems

Subsystem #Specific
Choline and Betaine Uptake and Betaine Biosynthesis 3
Lactate utilization 3
L-Arabinose utilization 2
Oxidative stress 2
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 2
Sialic Acid Metabolism 2
Thiamin biosynthesis 2
Choline Transport 1
D-galactonate catabolism 1
DNA-replication 1
DNA Repair Base Excision 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
Ketoisovalerate oxidoreductase 1
L-rhamnose utilization 1
Leucine Biosynthesis 1
Methylcitrate cycle 1
Nitrate and nitrite ammonification 1
Propionate-CoA to Succinate Module 1
Purine Utilization 1
Soluble cytochromes and functionally related electron carriers 1
Sulfur oxidation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
choline degradation I 2 2 2
glycine betaine biosynthesis I (Gram-negative bacteria) 2 2 2
glycine betaine biosynthesis II (Gram-positive bacteria) 2 2 2
pyruvate fermentation to (S)-lactate 1 1 1
acetate and ATP formation from acetyl-CoA III 1 1 1
acetate conversion to acetyl-CoA 1 1 1
choline-O-sulfate degradation 3 3 2
D-arabinose degradation I 2 2 1
nitrate reduction IV (dissimilatory) 2 2 1
pyrimidine nucleobases salvage II 2 2 1
pyrimidine ribonucleosides salvage III 2 2 1
β-alanine biosynthesis I 2 1 1
β-alanine biosynthesis IV 2 1 1
ribitol degradation I 2 1 1
2-methylcitrate cycle I 5 5 2
cardiolipin biosynthesis II 3 3 1
superpathway of acetate utilization and formation 3 3 1
cardiolipin biosynthesis I 3 3 1
2-methylcitrate cycle II 6 4 2
ethanol degradation IV 3 2 1
L-isoleucine biosynthesis V 3 2 1
ethanol degradation II 3 2 1
formate to nitrite electron transfer 3 2 1
glycine betaine biosynthesis III (plants) 3 2 1
D-arabinose degradation V 3 1 1
ethanol degradation III 3 1 1
dimethylsulfoniopropanoate biosynthesis I (Wollastonia) 3 1 1
N-acetylneuraminate and N-acetylmannosamine degradation I 4 4 1
L-arabinose degradation I 4 4 1
pyruvate fermentation to acetate and (S)-lactate I 4 4 1
pyruvate fermentation to acetate and lactate II 4 4 1
chitin deacetylation 4 3 1
cardiolipin and phosphatidylethanolamine biosynthesis (Xanthomonas) 4 3 1
choline degradation IV 4 2 1
dimethylsulfoniopropanoate biosynthesis II (Spartina) 4 1 1
superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation 6 6 1
L-isoleucine biosynthesis IV 6 4 1
β-alanine biosynthesis II 6 2 1
superpathway of bitter acids biosynthesis 18 3 3
colupulone and cohumulone biosynthesis 6 1 1
adlupulone and adhumulone biosynthesis 6 1 1
lupulone and humulone biosynthesis 6 1 1
superpathway of cardiolipin biosynthesis (bacteria) 13 11 2
reductive glycine pathway of autotrophic CO2 fixation 9 6 1
cis-geranyl-CoA degradation 9 1 1
superpathway of coenzyme A biosynthesis II (plants) 10 6 1
homolactic fermentation 12 12 1
superpathway of phospholipid biosynthesis III (E. coli) 12 12 1
(S)-lactate fermentation to propanoate, acetate and hydrogen 13 10 1
Bifidobacterium shunt 15 13 1
superpathway of glucose and xylose degradation 17 16 1
heterolactic fermentation 18 15 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 19 1
superpathway of anaerobic sucrose degradation 19 17 1
superpathway of pentose and pentitol degradation 42 15 2
superpathway of N-acetylneuraminate degradation 22 22 1