Experiment set6S105 for Escherichia coli BL21

Compare to:

Bas51

Group: phage
Media: LB_plus_SM_buffer + MOI 10
Culturing: BL21_ML4, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 9/20/23
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 2 genes in this experiment

For phage MOI 10 in Escherichia coli BL21

For phage MOI 10 across organisms

SEED Subsystems

Subsystem #Specific
Glycine and Serine Utilization 1
Glycine cleavage system 1
Photorespiration (oxidative C2 cycle) 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
glycine biosynthesis II 3 3 1
glycine degradation 3 3 1
glycine cleavage 3 3 1
folate transformations III (E. coli) 9 9 1
reductive glycine pathway of autotrophic CO2 fixation 9 6 1
photorespiration III 9 5 1
photorespiration I 9 5 1
photorespiration II 10 6 1
folate transformations II (plants) 11 10 1
folate transformations I 13 9 1