Experiment set6S105 for Escherichia coli BL21
Bas51
Group: phageMedia: LB_plus_SM_buffer + MOI 10
Culturing: BL21_ML4, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 9/20/23
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 2 genes in this experiment
For phage MOI 10 in Escherichia coli BL21
For phage MOI 10 across organisms
SEED Subsystems
Subsystem | #Specific |
---|---|
Glycine and Serine Utilization | 1 |
Glycine cleavage system | 1 |
Photorespiration (oxidative C2 cycle) | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
Pathway | #Steps | #Present | #Specific |
---|---|---|---|
glycine biosynthesis II | 3 | 3 | 1 |
glycine degradation | 3 | 3 | 1 |
glycine cleavage | 3 | 3 | 1 |
folate transformations III (E. coli) | 9 | 9 | 1 |
reductive glycine pathway of autotrophic CO2 fixation | 9 | 6 | 1 |
photorespiration III | 9 | 5 | 1 |
photorespiration I | 9 | 5 | 1 |
photorespiration II | 10 | 6 | 1 |
folate transformations II (plants) | 11 | 10 | 1 |
folate transformations I | 13 | 9 | 1 |