Experiment set6IT074 for Escherichia coli BW25113

Compare to:

inner cut, LB soft agar motility assay

200 most important genes:

  gene name fitness t score description  
b3979 thrT -5.5 -1.0 tRNA-Thr (NCBI) compare
b0222 lpcA -4.8 -5.3 phosphoheptose isomerase (NCBI) compare
b2330 yfcB -4.7 -2.2 putative adenine-specific methylase (VIMSS) compare
b0232 yafN -4.5 -1.2 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b3390 aroK -4.5 -3.1 shikimate kinase I (NCBI) compare
b3386 rpe -3.9 -4.1 ribulose-phosphate 3-epimerase (NCBI) compare
b3811 xerC -3.8 -5.8 site-specific tyrosine recombinase XerC (NCBI) compare
b2830 nudH -3.7 -2.4 dinucleoside polyphosphate hydrolase (NCBI) compare
b2620 smpB -3.6 -2.1 SsrA-binding protein (NCBI) compare
b0630 lipB -3.5 -2.9 protein of lipoate biosynthesis (VIMSS) compare
b3500 gor -3.5 -2.6 glutathione reductase (NCBI) compare
b1161 ycgX -3.4 -1.8 hypothetical protein (NCBI) compare
b2699 recA -3.3 -3.5 recombinase A (NCBI) compare
b3725 pstB -3.3 -5.5 phosphate transporter subunit (NCBI) compare
b1545 pinQ -3.2 -1.2 Qin prophage; predicted site-specific recombinase (NCBI) compare
b3483 yhhH -3.1 -1.3 orf, hypothetical protein (VIMSS) compare
b0118 acnB -3.1 -5.2 aconitate hydratase (NCBI) compare
b3121 yhaC -3.1 -2.3 hypothetical protein (NCBI) compare
b3463 ftsE -3.0 -6.9 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b4279 yjhB -3.0 -3.3 putative transport protein (VIMSS) compare
b0200 gmhB -3.0 -3.1 hypothetical protein (NCBI) compare
b1850 eda -2.9 -2.4 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b0738 tolR -2.8 -5.2 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0603 ybdO -2.8 -3.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b3621 rfaC -2.7 -7.7 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b1001 yccE -2.7 -3.1 hypothetical protein (NCBI) compare
b2567 rnc -2.6 -2.0 ribonuclease III (NCBI) compare
b4172 hfq -2.6 -2.2 RNA-binding protein Hfq (NCBI) compare
b4375 prfC -2.6 -8.5 peptide chain release factor 3 (NCBI) compare
b4402 yjjY -2.5 -1.3 hypothetical protein (NCBI) compare
b4253 yjgL -2.5 -7.1 orf, hypothetical protein (VIMSS) compare
b3741 gidA -2.5 -10.2 glucose-inhibited division protein A (NCBI) compare
b1882 cheY -2.5 -1.1 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1497 b1497 -2.5 -3.6 putative enzyme (VIMSS) compare
b0890 ftsK -2.4 -9.5 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b1363 trkG -2.4 -4.1 Rac prophage; potassium transporter subunit (NCBI) compare
b1505 ydeT -2.3 -5.4 hypothetical protein (RefSeq) compare
b2576 srmB -2.3 -5.0 ATP-dependent RNA helicase (NCBI) compare
b3609 secB -2.3 -3.1 export protein SecB (NCBI) compare
b0888 trxB -2.2 -4.1 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b3290 trkA -2.2 -4.9 potassium transporter peripheral membrane component (NCBI) compare
b3162 deaD -2.2 -6.4 inducible ATP-independent RNA helicase (VIMSS) compare
b3620 rfaF -2.2 -5.3 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b2822 recC -2.2 -6.1 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b1165 ymgA -2.1 -3.7 hypothetical protein (NCBI) compare
b1070 flgN -2.1 -0.9 export chaperone for FlgK and FlgL (NCBI) compare
b3619 rfaD -2.1 -4.5 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b1286 rnb -2.1 -3.9 exoribonuclease II (NCBI) compare
b1677 lpp -2.1 -2.8 murein lipoprotein (NCBI) compare
b3792 wzxE -2.1 -7.4 O-antigen translocase (NCBI) compare
b3164 pnp -2.1 -1.7 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b1257 yciE -2.0 -2.3 hypothetical protein (NCBI) compare
b3462 ftsX -2.0 -6.0 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1236 galU -2.0 -5.1 glucose-1-phosphate uridylyltransferase (NCBI) compare
b4577 sgrS -2.0 -1.1 small antisense RNA (NCBI) compare
b1712 ihfA -2.0 -3.1 integration host factor subunit alpha (NCBI) compare
b4458 oxyS -2.0 -0.9 global regulatory RNA (NCBI) compare
b0688 pgm -1.9 -3.2 phosphoglucomutase (NCBI) compare
b0439 lon -1.9 -6.0 DNA-binding ATP-dependent protease La (NCBI) compare
b0500 ybbD -1.9 -1.5 hypothetical protein (RefSeq) compare
b3778 rep -1.9 -7.7 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b1946 fliN -1.9 -2.8 flagellar motor switch protein (NCBI) compare
b2947 gshB -1.8 -4.4 glutathione synthetase (NCBI) compare
b1058 yceO -1.8 -1.3 hypothetical protein (NCBI) compare
b0082 mraW -1.8 -4.3 S-adenosyl-methyltransferase (NCBI) compare
b0741 pal -1.8 -2.9 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b2808 gcvA -1.8 -4.4 DNA-binding transcriptional dual regulator (NCBI) compare
b3839 tatC -1.8 -7.5 TatABCE protein translocation system subunit (NCBI) compare
b1167 ymgC -1.8 -1.3 hypothetical protein (NCBI) compare
b3933 ftsN -1.8 -9.5 essential cell division protein (NCBI) compare
b1362 rzpR -1.8 -2.8 putative Rac prophage endopeptidase (VIMSS) compare
b3504 yhiS -1.8 -2.2 hypothetical protein (RefSeq) compare
b4631 ykgQ -1.8 -1.1 no description compare
b0949 uup -1.8 -5.0 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b3631 rfaG -1.7 -11.2 glucosyltransferase I (NCBI) compare
b4571 wbbL -1.7 -4.0 no description compare
b2659 b2659 -1.7 -2.0 orf, hypothetical protein (VIMSS) compare
b0740 tolB -1.7 -4.4 translocation protein TolB precursor (NCBI) compare
b4239 treC -1.7 -5.1 trehalose-6-P hydrolase (NCBI) compare
b4066 yjcF -1.7 -1.8 hypothetical protein (NCBI) compare
b4704 arrS -1.7 -1.0 Antisense sRNA ArrS, function unknown (NCBI) compare
b4128 yjdK -1.7 -3.2 hypothetical protein (NCBI) compare
b0114 aceE -1.7 -2.6 pyruvate dehydrogenase subunit E1 (NCBI) compare
b0722 sdhD -1.7 -3.2 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b4416 rybA -1.6 -1.0 unknown RNA (NCBI) compare
b3791 rffA -1.6 -5.8 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b3706 trmE -1.6 -5.6 tRNA modification GTPase (NCBI) compare
b3237 argR -1.6 -3.7 arginine repressor (NCBI) compare
b1074 flgC -1.6 -1.1 flagellar basal-body rod protein C (NCBI) compare
b1292 sapC -1.6 -2.6 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0724 sdhB -1.6 -6.5 succinate dehydrogenase, FeS subunit (NCBI) compare
b4177 purA -1.6 -4.2 adenylosuccinate synthetase (NCBI) compare
b4481 rffT -1.6 -5.6 4-alpha-L-fucosyltransferase (NCBI) compare
b1073 flgB -1.6 -2.4 flagellar basal-body rod protein B (NCBI) compare
b2935 tktA -1.6 -2.8 transketolase 1, thiamin-binding (NCBI) compare
b1307 pspD -1.6 -1.1 peripheral inner membrane phage-shock protein (NCBI) compare
b1860 ruvB -1.6 -1.6 Holliday junction DNA helicase B (NCBI) compare
b3512 GadE -1.6 -2.2 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b2318 truA -1.6 -3.0 tRNA pseudouridine synthase A (NCBI) compare
b1160 elbA -1.6 -1.2 hypothetical protein (NCBI) compare
b1950 fliR -1.6 -4.8 flagellar biosynthesis protein R (NCBI) conserved
b0014 dnaK -1.6 -1.7 molecular chaperone DnaK (NCBI) compare
b3198 kdsC -1.6 -1.8 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b2175 spr -1.6 -2.9 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b3027 ygiZ -1.6 -1.9 conserved inner membrane protein (NCBI) compare
b3387 dam -1.6 -6.8 DNA adenine methylase (NCBI) compare
b0279 yagM -1.6 -3.0 CP4-6 prophage; predicted protein (NCBI) compare
b2948 yqgE -1.5 -2.7 orf, hypothetical protein (VIMSS) compare
b3806 cyaA -1.5 -5.5 adenylate cyclase (NCBI) compare
b3863 polA -1.5 -1.1 DNA polymerase I (NCBI) compare
b0739 tolA -1.5 -3.0 cell envelope integrity inner membrane protein TolA (NCBI) compare
b3630 rfaP -1.5 -8.3 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b3026 qseC -1.5 -4.0 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b2847 yqeI -1.5 -3.9 predicted transcriptional regulator (NCBI) compare
b1465 narV -1.5 -1.4 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b2287 nuoB -1.5 -2.7 NADH dehydrogenase subunit B (NCBI) compare
b3790 rffC -1.5 -3.7 TDP-fucosamine acetyltransferase (RefSeq) compare
b2501 ppk -1.5 -5.8 polyphosphate kinase (NCBI) compare
b2652 ileY -1.5 -2.4 tRNA-Ile (NCBI) compare
b1939 fliG -1.5 -2.8 flagellar motor switch protein G (NCBI) compare
b0617 citD -1.4 -1.4 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b2897 ygfY -1.4 -2.9 hypothetical protein (NCBI) compare
b2277 nuoM -1.4 -5.9 NADH dehydrogenase subunit M (NCBI) compare
b1891 flhC -1.4 -2.3 DNA-binding transcriptional dual regulator with FlhD (NCBI) compare
b4590 ybfK -1.4 -3.0 hypothetical protein (RefSeq) compare
b0326 yahL -1.4 -5.0 hypothetical protein (NCBI) compare
b2859 ygeO -1.4 -3.1 hypothetical protein (RefSeq) compare
b2432 yfeY -1.4 -2.0 hypothetical protein (NCBI) compare
b3120 yhaB -1.4 -1.8 orf, hypothetical protein (VIMSS) compare
b3940 metL -1.4 -5.8 bifunctional aspartate kinase II/homoserine dehydrogenase II (NCBI) compare
b1187 fadR -1.4 -2.3 fatty acid metabolism regulator (NCBI) compare
b4234 yjgA -1.4 -2.9 hypothetical protein (NCBI) compare
b2281 nuoI -1.4 -2.9 NADH dehydrogenase subunit I (NCBI) compare
b0723 sdhA -1.4 -7.2 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b2007 yeeX -1.4 -2.3 conserved protein (RefSeq) compare
b3169 nusA -1.4 -1.6 transcription elongation factor NusA (NCBI) compare
b0051 ksgA -1.4 -3.6 dimethyladenosine transferase (NCBI) compare
b1293 sapB -1.3 -1.4 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3442 yhhZ -1.3 -1.4 hypothetical protein (NCBI) compare
b0737 tolQ -1.3 -2.5 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0523 purE -1.3 -2.4 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b2614 grpE -1.3 -1.1 heat shock protein (NCBI) compare
b3870 glnA -1.3 -2.5 glutamine synthetase (NCBI) compare
b1266 yciV -1.3 -2.5 hypothetical protein (NCBI) compare
b3842 rfaH -1.3 -2.8 transcriptional activator RfaH (NCBI) compare
b2820 recB -1.3 -2.7 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b1075 flgD -1.3 -1.9 flagellar basal body rod modification protein D (NCBI) compare
b2283 nuoG -1.3 -6.0 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b3052 rfaE -1.3 -3.0 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b2055 wcaE -1.2 -1.8 predicted glycosyl transferase (NCBI) compare
b0767 ybhE -1.2 -2.7 6-phosphogluconolactonase (NCBI) compare
b3035 tolC -1.2 -4.3 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b2625 yfjI -1.2 -3.6 CP4-57 prophage; predicted protein (NCBI) compare
b2110 yehC -1.2 -1.8 predicted periplasmic pilin chaperone (NCBI) compare
b1072 flgA -1.2 -2.5 flagellar basal body P-ring biosynthesis protein A (NCBI) compare
b4593 ymgI -1.2 -2.2 hypothetical protein (RefSeq) compare
b1540 ydfH -1.2 -1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b0080 fruR -1.2 -4.3 DNA-binding transcriptional dual regulator (NCBI) compare
b0122 yacC -1.2 -2.5 orf, hypothetical protein (VIMSS) compare
b4191 ulaR -1.2 -2.3 DNA-binding transcriptional dual regulator (NCBI) compare
b3183 obgE -1.2 -0.8 GTPase involved in cell partioning and DNA repair (NCBI) compare
b1084 rne -1.2 -3.6 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b1576 ydfD -1.2 -1.4 Qin prophage; predicted protein (NCBI) compare
b4552 yrhC -1.2 -0.8 no description compare
b3636 rpmG -1.2 -1.2 50S ribosomal protein L33 (NCBI) compare
b0721 sdhC -1.2 -2.6 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b2579 yfiD -1.2 -1.4 pyruvate formate lyase subunit (NCBI) compare
b2934 cmtB -1.2 -1.7 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1949 fliQ -1.2 -1.7 flagellar biosynthesis protein Q (NCBI) compare
b1943 fliK -1.2 -3.2 flagellar hook-length control protein (NCBI) compare
b4373 rimI -1.2 -1.6 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b1175 minD -1.2 -4.3 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b3398 yrfF -1.2 -2.8 predicted inner membrane protein (NCBI) compare
b0802 ybiJ -1.2 -1.3 hypothetical protein (NCBI) compare
b2894 xerD -1.2 -1.3 site-specific tyrosine recombinase XerD (NCBI) compare
b0683 fur -1.1 -1.0 ferric uptake regulator (NCBI) compare
b2282 nuoH -1.1 -2.1 NADH dehydrogenase subunit H (NCBI) compare
b3652 recG -1.1 -5.1 ATP-dependent DNA helicase (NCBI) compare
b0430 cyoC -1.1 -3.1 cytochrome o ubiquinol oxidase subunit III (NCBI) compare
b1159 mcrA -1.1 -2.4 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b3002 yqhA -1.1 -1.3 hypothetical protein (NCBI) compare
b2846 yqeH -1.1 -0.6 orf, hypothetical protein (VIMSS) compare
b1890 motA -1.1 -1.9 flagellar motor protein MotA (NCBI) compare
b3236 mdh -1.1 -4.9 malate dehydrogenase (NCBI) compare
b4418 sraB -1.1 -1.1 unknown RNA (NCBI) compare
b2071 yegJ -1.1 -1.7 hypothetical protein (NCBI) compare
b2286 nuoC -1.1 -4.5 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b2051 wcaH -1.1 -0.9 GDP-mannose mannosyl hydrolase (VIMSS) compare
b0726 sucA -1.1 -3.3 alpha-ketoglutarate decarboxylase (NCBI) compare
b2218 rcsC -1.1 -5.4 hybrid sensory kinase in two-component regulatory system with RcsB and YojN (RefSeq) conserved
b4139 aspA -1.1 -5.5 aspartate ammonia-lyase (aspartase) (VIMSS) compare
b0605 ahpC -1.1 -2.7 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b1048 mdoG -1.1 -3.6 glucan biosynthesis protein, periplasmic (NCBI) compare
b0406 tgt -1.1 -3.1 queuine tRNA-ribosyltransferase (NCBI) compare
b4146 yjeK -1.1 -2.8 predicted lysine aminomutase (NCBI) compare
b1182 hlyE -1.0 -2.2 hemolysin E (VIMSS) compare
b4509 ylcG -1.0 -1.7 DLP12 prophage; predicted protein (NCBI) compare
b1948 fliP -1.0 -2.4 flagellar biosynthesis protein P (NCBI) compare
b1258 yciF -1.0 -2.3 hypothetical protein (NCBI) compare
b4215 ytfI -1.0 -3.2 hypothetical protein (NCBI) compare


Specific Phenotypes

For 3 genes in this experiment

For motility Agar in Escherichia coli BW25113

For motility Agar across organisms