Experiment set6IT073 for Escherichia coli BW25113

Compare to:

inner cut, LB soft agar motility assay

200 most important genes:

  gene name fitness t score description  
b3183 obgE -5.1 -1.8 GTPase involved in cell partioning and DNA repair (NCBI) compare
b0500 ybbD -5.1 -2.3 hypothetical protein (RefSeq) compare
b2330 yfcB -4.9 -2.2 putative adenine-specific methylase (VIMSS) compare
b3121 yhaC -4.3 -2.0 hypothetical protein (NCBI) compare
b2620 smpB -4.1 -2.3 SsrA-binding protein (NCBI) compare
b3979 thrT -4.0 -1.0 tRNA-Thr (NCBI) compare
b3483 yhhH -4.0 -1.3 orf, hypothetical protein (VIMSS) compare
b4172 hfq -3.8 -2.6 RNA-binding protein Hfq (NCBI) compare
b2935 tktA -3.7 -6.3 transketolase 1, thiamin-binding (NCBI) compare
b3463 ftsE -3.7 -8.5 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b4375 prfC -3.7 -11.1 peptide chain release factor 3 (NCBI) compare
b2830 nudH -3.6 -2.7 dinucleoside polyphosphate hydrolase (NCBI) compare
b3442 yhhZ -3.6 -3.0 hypothetical protein (NCBI) compare
b0200 gmhB -3.5 -3.4 hypothetical protein (NCBI) compare
b3169 nusA -3.5 -3.8 transcription elongation factor NusA (NCBI) compare
b2894 xerD -3.5 -2.2 site-specific tyrosine recombinase XerD (NCBI) compare
b3725 pstB -3.4 -5.4 phosphate transporter subunit (NCBI) compare
b1058 yceO -3.4 -1.4 hypothetical protein (NCBI) compare
b2820 recB -3.3 -5.2 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b0738 tolR -3.3 -4.8 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3863 polA -3.2 -2.1 DNA polymerase I (NCBI) compare
b0014 dnaK -3.1 -2.2 molecular chaperone DnaK (NCBI) compare
b0303 ykgI -3.1 -3.8 orf, hypothetical protein (VIMSS) compare
b3386 rpe -3.0 -3.8 ribulose-phosphate 3-epimerase (NCBI) compare
b3504 yhiS -2.9 -3.8 hypothetical protein (RefSeq) compare
b1236 galU -2.8 -6.8 glucose-1-phosphate uridylyltransferase (NCBI) compare
b2110 yehC -2.8 -2.8 predicted periplasmic pilin chaperone (NCBI) compare
b3027 ygiZ -2.8 -2.8 conserved inner membrane protein (NCBI) compare
b2567 rnc -2.7 -2.0 ribonuclease III (NCBI) compare
b2650 ypjC -2.7 -1.0 hypothetical protein (RefSeq) compare
b3811 xerC -2.6 -4.2 site-specific tyrosine recombinase XerC (NCBI) compare
b3778 rep -2.6 -11.2 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b3462 ftsX -2.6 -7.6 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0890 ftsK -2.5 -10.8 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b3621 rfaC -2.5 -8.4 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3792 wzxE -2.4 -7.4 O-antigen translocase (NCBI) compare
b0949 uup -2.4 -8.0 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b2168 fruK -2.4 -1.9 1-phosphofructokinase (NCBI) compare
b2847 yqeI -2.3 -6.6 predicted transcriptional regulator (NCBI) compare
b4042 dgkA -2.3 -3.5 diacylglycerol kinase (NCBI) compare
b1545 pinQ -2.3 -1.1 Qin prophage; predicted site-specific recombinase (NCBI) compare
b3630 rfaP -2.3 -13.0 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b0683 fur -2.3 -1.6 ferric uptake regulator (NCBI) compare
b0118 acnB -2.3 -4.5 aconitate hydratase (NCBI) compare
b2699 recA -2.3 -2.7 recombinase A (NCBI) compare
b0439 lon -2.3 -6.2 DNA-binding ATP-dependent protease La (NCBI) compare
b4557 yidD -2.3 -3.0 hypothetical protein (NCBI) compare
b3390 aroK -2.3 -2.2 shikimate kinase I (NCBI) compare
b0726 sucA -2.2 -3.8 alpha-ketoglutarate decarboxylase (NCBI) compare
b1347 ydaC -2.2 -1.8 Rac prophage; predicted protein (NCBI) compare
b4177 purA -2.2 -5.4 adenylosuccinate synthetase (NCBI) compare
b4279 yjhB -2.2 -3.2 putative transport protein (VIMSS) compare
b4410 ecnA -2.2 -0.8 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b3842 rfaH -2.2 -5.0 transcriptional activator RfaH (NCBI) compare
b0603 ybdO -2.1 -3.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b4497 yeeL -2.1 -4.4 no description compare
b1505 ydeT -2.1 -4.0 hypothetical protein (RefSeq) compare
b3631 rfaG -2.1 -14.2 glucosyltransferase I (NCBI) compare
b0222 lpcA -2.1 -3.3 phosphoheptose isomerase (NCBI) compare
b4592 yccB -2.1 -1.5 hypothetical protein (RefSeq) compare
b4704 arrS -2.1 -1.3 Antisense sRNA ArrS, function unknown (NCBI) compare
b1961 dcm -2.0 -1.3 DNA cytosine methylase (NCBI) compare
b2939 yqgB -2.0 -1.4 predicted protein (RefSeq) compare
b4402 yjjY -2.0 -1.1 hypothetical protein (NCBI) compare
b1850 eda -2.0 -2.3 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b3620 rfaF -2.0 -5.4 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b2576 srmB -2.0 -3.6 ATP-dependent RNA helicase (NCBI) compare
b1231 tyrT -2.0 -0.8 tRNA-Tyr (NCBI) compare
b2853 ygeI -1.9 -2.6 hypothetical protein (NCBI) compare
b3916 pfkA -1.9 -4.9 6-phosphofructokinase (NCBI) compare
b3026 qseC -1.9 -5.6 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b1499 ydeO -1.9 -2.4 predicted DNA-binding transcriptional acfivator (NCBI) compare
b1831 proQ -1.9 -4.1 putative solute/DNA competence effector (NCBI) compare
b4239 treC -1.9 -6.3 trehalose-6-P hydrolase (NCBI) compare
b3052 rfaE -1.9 -4.0 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b1861 ruvA -1.9 -1.8 Holliday junction DNA helicase motor protein (NCBI) compare
b3609 secB -1.9 -1.7 export protein SecB (NCBI) compare
b1434 ydcN -1.8 -1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b2569 lepA -1.8 -4.6 GTP-binding protein LepA (NCBI) compare
b2288 nuoA -1.8 -2.6 NADH dehydrogenase I chain A (VIMSS) compare
b2501 ppk -1.8 -8.4 polyphosphate kinase (NCBI) compare
b0740 tolB -1.8 -4.2 translocation protein TolB precursor (NCBI) compare
b2822 recC -1.8 -4.7 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b2947 gshB -1.8 -4.2 glutathione synthetase (NCBI) compare
b3619 rfaD -1.8 -3.9 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3933 ftsN -1.8 -8.7 essential cell division protein (NCBI) compare
b0688 pgm -1.8 -3.4 phosphoglucomutase (NCBI) compare
b0722 sdhD -1.8 -3.3 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b4414 tff -1.8 -2.3 unknown RNA (NCBI) compare
b4345 mcrC -1.7 -2.8 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b4045 yjbJ -1.7 -1.8 predicted stress response protein (NCBI) compare
b3741 gidA -1.7 -6.8 glucose-inhibited division protein A (NCBI) compare
b4201 priB -1.7 -2.6 primosomal replication protein N (NCBI) compare
b1551 b1551 -1.7 -1.6 orf, hypothetical protein (VIMSS) compare
b1257 yciE -1.7 -2.3 hypothetical protein (NCBI) compare
b3162 deaD -1.7 -4.5 inducible ATP-independent RNA helicase (VIMSS) compare
b1292 sapC -1.7 -2.6 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0737 tolQ -1.7 -3.0 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0082 mraW -1.7 -2.6 S-adenosyl-methyltransferase (NCBI) compare
b0866 ybjQ -1.7 -0.8 hypothetical protein (NCBI) compare
b1730 ydjO -1.7 -2.0 predicted protein (RefSeq) compare
b1950 fliR -1.7 -4.5 flagellar biosynthesis protein R (NCBI) conserved
b0739 tolA -1.6 -3.4 cell envelope integrity inner membrane protein TolA (NCBI) compare
b1941 fliI -1.6 -5.9 flagellum-specific ATP synthase (NCBI) conserved
b3838 tatB -1.6 -5.5 sec-independent translocase (NCBI) compare
b3806 cyaA -1.6 -5.5 adenylate cyclase (NCBI) compare
b1860 ruvB -1.6 -1.3 Holliday junction DNA helicase B (NCBI) compare
b3035 tolC -1.6 -7.1 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b1048 mdoG -1.6 -5.4 glucan biosynthesis protein, periplasmic (NCBI) compare
b3198 kdsC -1.6 -2.4 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b3791 rffA -1.6 -7.3 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b3839 tatC -1.6 -4.8 TatABCE protein translocation system subunit (NCBI) compare
b1361 ydaW -1.5 -0.9 orf, hypothetical protein (VIMSS) compare
b3871 bipA -1.5 -7.7 GTP-binding protein (NCBI) compare
b2318 truA -1.5 -3.5 tRNA pseudouridine synthase A (NCBI) compare
b1830 prc -1.5 -5.8 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b0888 trxB -1.5 -3.1 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b4000 hupA -1.5 -3.4 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b0741 pal -1.5 -2.3 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b4128 yjdK -1.5 -2.5 hypothetical protein (NCBI) compare
b3706 trmE -1.5 -4.1 tRNA modification GTPase (NCBI) compare
b3977 tyrU -1.5 -0.8 tRNA-Tyr (NCBI) compare
b2363 yfdT -1.5 -2.2 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b1160 elbA -1.5 -1.8 hypothetical protein (NCBI) compare
b3199 yrbK -1.5 -2.1 hypothetical protein (NCBI) compare
b2897 ygfY -1.5 -2.7 hypothetical protein (NCBI) compare
b1080 flgI -1.5 -5.6 homolog of Salmonella P-ring of flagella basal body (VIMSS) conserved
b2642 yfjW -1.5 -1.5 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b1948 fliP -1.5 -5.1 flagellar biosynthesis protein P (NCBI) compare
b1583 ynfB -1.4 -2.7 hypothetical protein (NCBI) compare
b2216 rcsD -1.4 -8.1 phosphotransfer intermediate protein in two-component regulatory system with RcsBC (NCBI) compare
b1187 fadR -1.4 -2.7 fatty acid metabolism regulator (NCBI) compare
b1879 flhA -1.4 -5.5 flagellar biosynthesis protein A (NCBI) conserved
b3387 dam -1.4 -8.2 DNA adenine methylase (NCBI) compare
b1923 fliC -1.4 -5.7 flagellin (NCBI) compare
b1073 flgB -1.4 -3.9 flagellar basal-body rod protein B (NCBI) compare
b4066 yjcF -1.4 -3.0 hypothetical protein (NCBI) compare
b4660 yhiL -1.4 -1.7 no description compare
b3761 trpT -1.4 -0.8 tRNA-Trp (NCBI) compare
b1892 flhD -1.4 -4.9 DNA-binding transcriptional dual regulator with FlhC (RefSeq) compare
b2281 nuoI -1.4 -2.5 NADH dehydrogenase subunit I (NCBI) compare
b1290 sapF -1.4 -1.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1922 fliA -1.4 -3.8 flagellar biosynthesis; alternative sigma factor 28; regulation of flagellar operons (VIMSS) compare
b4481 rffT -1.4 -5.9 4-alpha-L-fucosyltransferase (NCBI) compare
b1167 ymgC -1.4 -1.1 hypothetical protein (NCBI) compare
b1412 azoR -1.4 -1.4 acyl carrier protein phosphodiesterase (NCBI) compare
b1049 mdoH -1.4 -7.0 glucosyltransferase MdoH (NCBI) compare
b2286 nuoC -1.4 -4.7 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b1497 b1497 -1.4 -1.9 putative enzyme (VIMSS) compare
b1077 flgF -1.4 -3.5 flagellar component of cell-proximal portion of basal-body rod (NCBI) compare
b1075 flgD -1.4 -2.5 flagellar basal body rod modification protein D (NCBI) compare
b3728 pstS -1.3 -4.5 phosphate transporter subunit (NCBI) compare
b3164 pnp -1.3 -2.1 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b1079 flgH -1.3 -3.6 flagellar L-ring protein precursor H (NCBI) compare
b1709 btuD -1.3 -1.0 vitamin B12-transporter ATPase (NCBI) compare
b3261 fis -1.3 -2.4 DNA-binding protein Fis (NCBI) compare
b2297 pta -1.3 -1.3 phosphate acetyltransferase (NCBI) compare
b1949 fliQ -1.3 -1.2 flagellar biosynthesis protein Q (NCBI) compare
b1946 fliN -1.3 -1.7 flagellar motor switch protein (NCBI) compare
b3613 yibP -1.3 -2.8 putative membrane protein (VIMSS) compare
b1176 minC -1.3 -2.2 septum formation inhibitor (NCBI) compare
b4578 insP -1.3 -1.7 no description compare
b1891 flhC -1.3 -2.1 DNA-binding transcriptional dual regulator with FlhD (NCBI) compare
b1804 rnd -1.3 -2.1 ribonuclease D (NCBI) compare
b1078 flgG -1.3 -5.2 flagellar component of cell-distal portion of basal-body rod (NCBI) compare
b1273 yciN -1.3 -1.6 hypothetical protein (NCBI) compare
b0232 yafN -1.3 -0.9 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b1095 fabF -1.3 -3.9 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b2614 grpE -1.2 -1.2 heat shock protein (NCBI) compare
b3787 rffD -1.2 -6.3 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b3002 yqhA -1.2 -1.3 hypothetical protein (NCBI) compare
b0310 ykgH -1.2 -2.3 predicted inner membrane protein (NCBI) compare
b3940 metL -1.2 -4.9 bifunctional aspartate kinase II/homoserine dehydrogenase II (NCBI) compare
b2051 wcaH -1.2 -1.0 GDP-mannose mannosyl hydrolase (VIMSS) compare
b2314 dedD -1.2 -1.9 putative lipoprotein (VIMSS) compare
b1940 fliH -1.2 -2.4 flagellar biosynthesis; export of flagellar proteins? (VIMSS) compare
b1074 flgC -1.2 -1.2 flagellar basal-body rod protein C (NCBI) compare
b1072 flgA -1.2 -3.4 flagellar basal body P-ring biosynthesis protein A (NCBI) compare
b0406 tgt -1.2 -3.7 queuine tRNA-ribosyltransferase (NCBI) compare
b4253 yjgL -1.2 -3.1 orf, hypothetical protein (VIMSS) compare
b1889 motB -1.2 -3.0 flagellar motor protein MotB (NCBI) conserved
b3616 tdh -1.2 -4.4 L-threonine 3-dehydrogenase (NCBI) compare
b1175 minD -1.2 -4.6 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b2937 speB -1.2 -4.5 agmatinase (NCBI) compare
b1880 flhB -1.2 -5.0 flagellar biosynthesis protein B (NCBI) conserved
b0294 ykgK -1.2 -3.9 predicted regulator (NCBI) compare
b1540 ydfH -1.2 -0.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b1925 fliS -1.2 -4.2 flagellar protein FliS (NCBI) conserved
b2808 gcvA -1.2 -3.9 DNA-binding transcriptional dual regulator (NCBI) compare
b4215 ytfI -1.2 -3.2 hypothetical protein (NCBI) compare
b0798 ybiA -1.2 -1.6 hypothetical protein (NCBI) compare
b2846 yqeH -1.2 -1.0 orf, hypothetical protein (VIMSS) compare
b4552 yrhC -1.2 -0.7 no description compare
b0723 sdhA -1.2 -5.6 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b4441 glmY -1.2 -0.9 sRNA activator of glmS mRNA (RefSeq) compare
b3250 mreC -1.2 -0.9 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b1888 cheA -1.2 -3.3 fused chemotactic sensory histidine kinase in two-component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein (NCBI) compare
b1658 purR -1.1 -4.8 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b0018 mokC -1.1 -0.6 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b1945 fliM -1.1 -2.4 flagellar motor switch protein M (NCBI) conserved


Specific Phenotypes

For 10 genes in this experiment

For motility Agar in Escherichia coli BW25113

For motility Agar across organisms