Experiment set6IT072 for Escherichia coli BW25113

Compare to:

inner cut, LB soft agar motility assay

200 most important genes:

  gene name fitness t score description  
b3027 ygiZ -5.3 -2.9 conserved inner membrane protein (NCBI) compare
b2567 rnc -4.8 -2.3 ribonuclease III (NCBI) compare
b3483 yhhH -4.7 -1.5 orf, hypothetical protein (VIMSS) compare
b4402 yjjY -4.6 -1.4 hypothetical protein (NCBI) compare
b2330 yfcB -4.4 -2.5 putative adenine-specific methylase (VIMSS) compare
b0683 fur -4.3 -2.0 ferric uptake regulator (NCBI) compare
b3386 rpe -4.2 -2.9 ribulose-phosphate 3-epimerase (NCBI) compare
b1882 cheY -4.0 -1.2 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b4443 gcvB -4.0 -2.0 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b0630 lipB -3.9 -3.2 protein of lipoate biosynthesis (VIMSS) compare
b1167 ymgC -3.9 -2.0 hypothetical protein (NCBI) compare
b0500 ybbD -3.8 -2.2 hypothetical protein (RefSeq) compare
b3162 deaD -3.7 -7.6 inducible ATP-independent RNA helicase (VIMSS) compare
b4704 arrS -3.6 -1.5 Antisense sRNA ArrS, function unknown (NCBI) compare
b1850 eda -3.6 -2.7 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b0737 tolQ -3.4 -5.5 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3725 pstB -3.3 -5.5 phosphate transporter subunit (NCBI) compare
b3463 ftsE -3.3 -8.3 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b3778 rep -3.3 -11.8 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b3164 pnp -3.3 -3.8 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b2297 pta -3.2 -2.7 phosphate acetyltransferase (NCBI) compare
b3842 rfaH -3.2 -6.6 transcriptional activator RfaH (NCBI) compare
b0222 lpcA -3.2 -4.7 phosphoheptose isomerase (NCBI) compare
b0439 lon -3.2 -8.8 DNA-binding ATP-dependent protease La (NCBI) compare
b3026 qseC -3.2 -7.5 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b0200 gmhB -3.1 -2.8 hypothetical protein (NCBI) compare
b2620 smpB -3.1 -2.3 SsrA-binding protein (NCBI) compare
b4375 prfC -3.1 -11.5 peptide chain release factor 3 (NCBI) compare
b2935 tktA -3.1 -5.7 transketolase 1, thiamin-binding (NCBI) compare
b1182 hlyE -3.0 -3.5 hemolysin E (VIMSS) compare
b2830 nudH -3.0 -2.7 dinucleoside polyphosphate hydrolase (NCBI) compare
b2110 yehC -3.0 -4.5 predicted periplasmic pilin chaperone (NCBI) compare
b3621 rfaC -3.0 -10.1 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b2699 recA -3.0 -3.1 recombinase A (NCBI) compare
b3811 xerC -2.9 -5.0 site-specific tyrosine recombinase XerC (NCBI) compare
b1860 ruvB -2.9 -2.4 Holliday junction DNA helicase B (NCBI) compare
b3290 trkA -2.8 -6.5 potassium transporter peripheral membrane component (NCBI) compare
b1075 flgD -2.8 -3.0 flagellar basal body rod modification protein D (NCBI) compare
b3741 gidA -2.8 -10.8 glucose-inhibited division protein A (NCBI) compare
b3977 tyrU -2.8 -1.0 tRNA-Tyr (NCBI) compare
b3121 yhaC -2.8 -2.3 hypothetical protein (NCBI) compare
b3390 aroK -2.7 -2.8 shikimate kinase I (NCBI) compare
b3504 yhiS -2.7 -2.8 hypothetical protein (RefSeq) compare
b3500 gor -2.7 -2.6 glutathione reductase (NCBI) compare
b1236 galU -2.7 -7.6 glucose-1-phosphate uridylyltransferase (NCBI) compare
b4042 dgkA -2.7 -4.6 diacylglycerol kinase (NCBI) compare
b3052 rfaE -2.7 -7.9 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b0740 tolB -2.7 -4.9 translocation protein TolB precursor (NCBI) compare
b2259 pmrD -2.7 -1.1 polymyxin resistance protein B (VIMSS) compare
b0739 tolA -2.7 -5.3 cell envelope integrity inner membrane protein TolA (NCBI) compare
b0014 dnaK -2.6 -2.2 molecular chaperone DnaK (NCBI) compare
b0888 trxB -2.6 -3.9 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b3979 thrT -2.6 -1.0 tRNA-Thr (NCBI) compare
b4201 priB -2.6 -2.1 primosomal replication protein N (NCBI) compare
b4651 yibW -2.5 -2.1 no description compare
b3806 cyaA -2.5 -9.1 adenylate cyclase (NCBI) compare
b3728 pstS -2.5 -6.8 phosphate transporter subunit (NCBI) compare
b0738 tolR -2.5 -4.1 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0232 yafN -2.5 -1.2 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b0890 ftsK -2.4 -9.2 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b4593 ymgI -2.4 -2.7 hypothetical protein (RefSeq) compare
b3169 nusA -2.4 -2.2 transcription elongation factor NusA (NCBI) compare
b3619 rfaD -2.4 -5.0 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b0118 acnB -2.4 -4.8 aconitate hydratase (NCBI) compare
b2897 ygfY -2.4 -2.5 hypothetical protein (NCBI) compare
b4177 purA -2.3 -5.2 adenylosuccinate synthetase (NCBI) compare
b3792 wzxE -2.3 -7.4 O-antigen translocase (NCBI) compare
b3609 secB -2.2 -3.5 export protein SecB (NCBI) compare
b1941 fliI -2.2 -7.1 flagellum-specific ATP synthase (NCBI) conserved
b4603 rseX -2.2 -1.0 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b3462 ftsX -2.2 -5.1 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b4279 yjhB -2.2 -3.3 putative transport protein (VIMSS) compare
b1891 flhC -2.2 -4.3 DNA-binding transcriptional dual regulator with FlhD (NCBI) compare
b2822 recC -2.2 -5.3 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b3198 kdsC -2.1 -3.5 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b3620 rfaF -2.1 -4.7 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b1001 yccE -2.1 -3.0 hypothetical protein (NCBI) compare
b4239 treC -2.1 -5.5 trehalose-6-P hydrolase (NCBI) compare
b2650 ypjC -2.1 -1.2 hypothetical protein (RefSeq) compare
b3613 yibP -2.1 -5.5 putative membrane protein (VIMSS) compare
b3183 obgE -2.1 -1.4 GTPase involved in cell partioning and DNA repair (NCBI) compare
b1804 rnd -2.0 -4.5 ribonuclease D (NCBI) compare
b3706 trmE -2.0 -7.4 tRNA modification GTPase (NCBI) compare
b1070 flgN -2.0 -1.0 export chaperone for FlgK and FlgL (NCBI) compare
b0741 pal -2.0 -3.8 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b1505 ydeT -2.0 -3.7 hypothetical protein (RefSeq) compare
b3512 GadE -1.9 -3.2 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b4416 rybA -1.9 -1.2 unknown RNA (NCBI) compare
b3631 rfaG -1.9 -12.8 glucosyltransferase I (NCBI) compare
b0605 ahpC -1.9 -3.0 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b2808 gcvA -1.9 -4.5 DNA-binding transcriptional dual regulator (NCBI) compare
b3630 rfaP -1.9 -9.5 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b1948 fliP -1.9 -3.6 flagellar biosynthesis protein P (NCBI) compare
b2071 yegJ -1.9 -2.6 hypothetical protein (NCBI) compare
b1545 pinQ -1.9 -1.1 Qin prophage; predicted site-specific recombinase (NCBI) compare
b1058 yceO -1.8 -1.3 hypothetical protein (NCBI) compare
b1925 fliS -1.8 -3.5 flagellar protein FliS (NCBI) conserved
b2953 yggU -1.8 -1.7 orf, hypothetical protein (VIMSS) compare
b1712 ihfA -1.8 -3.0 integration host factor subunit alpha (NCBI) compare
b3791 rffA -1.8 -6.2 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b3199 yrbK -1.8 -2.0 hypothetical protein (NCBI) compare
b4172 hfq -1.8 -1.8 RNA-binding protein Hfq (NCBI) compare
b1079 flgH -1.8 -2.4 flagellar L-ring protein precursor H (NCBI) compare
b0722 sdhD -1.8 -4.0 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b4481 rffT -1.7 -5.4 4-alpha-L-fucosyltransferase (NCBI) compare
b1257 yciE -1.7 -2.0 hypothetical protein (NCBI) compare
b1889 motB -1.7 -5.3 flagellar motor protein MotB (NCBI) conserved
b4202 rpsR -1.7 -1.9 30S ribosomal protein S18 (NCBI) compare
b1497 b1497 -1.7 -2.8 putative enzyme (VIMSS) compare
b0949 uup -1.7 -4.5 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b2894 xerD -1.7 -1.6 site-specific tyrosine recombinase XerD (NCBI) compare
b0051 ksgA -1.7 -4.8 dimethyladenosine transferase (NCBI) compare
b2847 yqeI -1.7 -5.3 predicted transcriptional regulator (NCBI) compare
b2820 recB -1.7 -3.3 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b2569 lepA -1.6 -4.5 GTP-binding protein LepA (NCBI) compare
b0912 ihfB -1.6 -2.4 integration host factor subunit beta (NCBI) compare
b1926 fliT -1.6 -1.9 predicted chaperone (NCBI) compare
b1082 flgK -1.6 -5.0 flagellar hook-associated protein K (NCBI) compare
b3261 fis -1.6 -2.6 DNA-binding protein Fis (NCBI) compare
b1160 elbA -1.6 -1.3 hypothetical protein (NCBI) compare
b3727 pstC -1.6 -4.8 phosphate transporter subunit (NCBI) compare
b3839 tatC -1.6 -5.1 TatABCE protein translocation system subunit (NCBI) compare
b3387 dam -1.5 -7.1 DNA adenine methylase (NCBI) compare
b2501 ppk -1.5 -6.3 polyphosphate kinase (NCBI) compare
b1252 tonB -1.5 -2.6 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b1409 ynbB -1.5 -4.2 predicted CDP-diglyceride synthase (NCBI) compare
b3442 yhhZ -1.5 -1.8 hypothetical protein (NCBI) compare
b1924 fliD -1.5 -4.0 flagellar capping protein (NCBI) compare
b1880 flhB -1.5 -3.7 flagellar biosynthesis protein B (NCBI) conserved
b2282 nuoH -1.5 -2.8 NADH dehydrogenase subunit H (NCBI) compare
b4253 yjgL -1.4 -4.3 orf, hypothetical protein (VIMSS) compare
b2528 iscA -1.4 -1.2 iron-sulfur cluster assembly protein (NCBI) compare
b0015 dnaJ -1.4 -5.4 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b4458 oxyS -1.4 -0.9 global regulatory RNA (NCBI) compare
b1078 flgG -1.4 -3.5 flagellar component of cell-distal portion of basal-body rod (NCBI) compare
b1048 mdoG -1.4 -4.9 glucan biosynthesis protein, periplasmic (NCBI) compare
b0406 tgt -1.4 -5.6 queuine tRNA-ribosyltransferase (NCBI) compare
b1121 ycfZ -1.4 -4.5 predicted inner membrane protein (NCBI) compare
b0746 valZ -1.4 -1.3 tRNA-Val (NCBI) compare
b1074 flgC -1.4 -1.3 flagellar basal-body rod protein C (NCBI) compare
b0866 ybjQ -1.4 -0.8 hypothetical protein (NCBI) compare
b4128 yjdK -1.4 -2.7 hypothetical protein (NCBI) compare
b3933 ftsN -1.4 -6.6 essential cell division protein (NCBI) compare
b4647 mokA -1.4 -1.5 no description compare
b0627 tatE -1.4 -1.8 twin arginine translocase protein E (NCBI) compare
b1879 flhA -1.3 -4.5 flagellar biosynthesis protein A (NCBI) conserved
b1400 paaY -1.3 -1.8 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b1269 rluB -1.3 -3.0 23S rRNA pseudouridylate synthase (NCBI) compare
b1007 b1007 -1.3 -1.0 orf, hypothetical protein (VIMSS) compare
b2625 yfjI -1.3 -2.5 CP4-57 prophage; predicted protein (NCBI) compare
b1572 ydfB -1.3 -0.9 orf, hypothetical protein (VIMSS) compare
b4590 ybfK -1.3 -3.4 hypothetical protein (RefSeq) compare
b2576 srmB -1.3 -2.8 ATP-dependent RNA helicase (NCBI) compare
b1607 ydgC -1.3 -2.0 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b2476 purC -1.3 -1.6 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b4345 mcrC -1.3 -2.6 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b0721 sdhC -1.3 -2.6 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b0723 sdhA -1.3 -5.5 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b0053 surA -1.3 -3.7 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b0623 cspE -1.3 -1.2 cold shock protein E (NCBI) compare
b2280 nuoJ -1.3 -4.5 NADH dehydrogenase subunit J (NCBI) compare
b1945 fliM -1.3 -3.7 flagellar motor switch protein M (NCBI) conserved
b2287 nuoB -1.3 -2.7 NADH dehydrogenase subunit B (NCBI) compare
b1888 cheA -1.2 -4.1 fused chemotactic sensory histidine kinase in two-component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein (NCBI) compare
b2853 ygeI -1.2 -1.8 hypothetical protein (NCBI) compare
b4557 yidD -1.2 -1.6 hypothetical protein (NCBI) compare
b0018 mokC -1.2 -0.6 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b3120 yhaB -1.2 -2.8 orf, hypothetical protein (VIMSS) compare
b1080 flgI -1.2 -3.3 homolog of Salmonella P-ring of flagella basal body (VIMSS) conserved
b3094 exuR -1.2 -3.7 negative regulator of exu regulon, exuT, uxaAC, and uxuB (VIMSS) compare
b1049 mdoH -1.2 -4.2 glucosyltransferase MdoH (NCBI) compare
b1095 fabF -1.2 -3.7 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b0279 yagM -1.2 -3.4 CP4-6 prophage; predicted protein (NCBI) compare
b2291 yfbR -1.2 -2.0 hypothetical protein (NCBI) compare
b0303 ykgI -1.2 -1.2 orf, hypothetical protein (VIMSS) compare
b1950 fliR -1.2 -3.3 flagellar biosynthesis protein R (NCBI) conserved
b3389 aroB -1.2 -4.7 3-dehydroquinate synthase (NCBI) compare
b1175 minD -1.2 -3.3 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b0247 ykfG -1.2 -0.7 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b0426 yajQ -1.2 -4.0 orf, hypothetical protein (VIMSS) compare
b1855 lpxM -1.2 -2.7 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b0082 mraW -1.2 -2.8 S-adenosyl-methyltransferase (NCBI) compare
b0389 yaiA -1.1 -1.5 hypothetical protein (NCBI) compare
b4139 aspA -1.1 -4.7 aspartate ammonia-lyase (aspartase) (VIMSS) compare
b3863 polA -1.1 -1.0 DNA polymerase I (NCBI) compare
b0585 fes -1.1 -3.6 enterobactin/ferric enterobactin esterase (NCBI) compare
b0726 sucA -1.1 -2.6 alpha-ketoglutarate decarboxylase (NCBI) compare
b1165 ymgA -1.1 -2.7 hypothetical protein (NCBI) compare
b2218 rcsC -1.1 -5.0 hybrid sensory kinase in two-component regulatory system with RcsB and YojN (RefSeq) conserved
b1084 rne -1.1 -2.9 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b4521 ycgI -1.1 -2.3 hypothetical protein (NCBI) compare
b1334 fnr -1.1 -2.0 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b1730 ydjO -1.1 -0.8 predicted protein (RefSeq) compare
b2948 yqgE -1.1 -1.7 orf, hypothetical protein (VIMSS) compare
b3119 tdcR -1.1 -1.4 threonine dehydratase operon activator protein (VIMSS) compare
b4699 fnrS -1.1 -0.7 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b2288 nuoA -1.1 -2.4 NADH dehydrogenase I chain A (VIMSS) compare
b0705 ybfL -1.1 -1.2 putative receptor protein (VIMSS) compare
b0122 yacC -1.1 -1.9 orf, hypothetical protein (VIMSS) compare
b2313 cvpA -1.1 -2.6 membrane protein required for colicin V production (NCBI) compare


Specific Phenotypes

For 5 genes in this experiment

For motility Agar in Escherichia coli BW25113

For motility Agar across organisms