Experiment set6IT071 for Pseudomonas putida KT2440
Sorghum Hydrolysate carbon source
Group: carbon sourceMedia: M9 minimal media_noCarbon + Sorghum Hydrolysate (28.6 vol%)
Culturing: Putida_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 6.1 generations
By: Mitch on 12/8/17
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride
Specific Phenotypes
For 1 genes in this experiment
For carbon source Sorghum Hydrolysate in Pseudomonas putida KT2440
For carbon source Sorghum Hydrolysate across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycine, serine and threonine metabolism
- Cyanoamino acid metabolism
- One carbon pool by folate
- Methane metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glycine biosynthesis I | 1 | 1 | 1 |
| glycine degradation | 3 | 3 | 1 |
| dTMP de novo biosynthesis (mitochondrial) | 3 | 3 | 1 |
| superpathway of L-serine and glycine biosynthesis I | 4 | 4 | 1 |
| glycine betaine degradation II (mammalian) | 4 | 1 | 1 |
| folate polyglutamylation | 5 | 4 | 1 |
| glycine betaine degradation III | 7 | 7 | 1 |
| glycine betaine degradation I | 8 | 6 | 1 |
| folate transformations III (E. coli) | 9 | 9 | 1 |
| photorespiration I | 9 | 6 | 1 |
| photorespiration III | 9 | 6 | 1 |
| photorespiration II | 10 | 7 | 1 |
| folate transformations II (plants) | 11 | 10 | 1 |
| folate transformations I | 13 | 9 | 1 |
| formaldehyde assimilation I (serine pathway) | 13 | 7 | 1 |
| purine nucleobases degradation II (anaerobic) | 24 | 16 | 1 |