Experiment set6IT068 for Pseudomonas putida KT2440

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Lignin Monomers carbon source

Group: carbon source
Media: M9 minimal media_noCarbon + Lignin Monomers (16.6 vol%)
Culturing: Putida_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 5.0 generations
By: Mitch on 12/8/17
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 11 genes in this experiment

For carbon source Lignin Monomers in Pseudomonas putida KT2440

For carbon source Lignin Monomers across organisms

SEED Subsystems

Subsystem #Specific
Aromatic amino acid degradation 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
Phosphate metabolism 1
Purine conversions 1
Serine-glyoxylate cycle 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
adenosine nucleotides degradation III 1 1 1
palmitoleate biosynthesis III (cyanobacteria) 2 1 1
phospholipid remodeling (phosphatidate, yeast) 2 1 1
oleate biosynthesis III (cyanobacteria) 3 2 1
CDP-diacylglycerol biosynthesis II 4 4 1
CDP-diacylglycerol biosynthesis I 4 4 1
CDP-diacylglycerol biosynthesis III 5 3 1
phosphatidate biosynthesis (yeast) 5 3 1
phosphatidylglycerol biosynthesis II 6 6 1
phosphatidylglycerol biosynthesis I 6 6 1
superpathway of phospholipid biosynthesis III (E. coli) 12 10 2
superpathway of stearidonate biosynthesis (cyanobacteria) 6 2 1
palmitoyl ethanolamide biosynthesis 6 2 1
diacylglycerol and triacylglycerol biosynthesis 7 3 1
stigma estolide biosynthesis 7 2 1
anandamide biosynthesis II 8 2 1
anandamide biosynthesis I 12 3 1
superpathway of cardiolipin biosynthesis (bacteria) 13 9 1
superpathway of phospholipid biosynthesis II (plants) 28 10 2
plasmalogen biosynthesis I (aerobic) 16 1 1