Experiment set6IT068 for Escherichia coli BW25113

Compare to:

outer cut, LB soft agar motility assay

200 most important genes:

  gene name fitness t score description  
b0683 fur -5.9 -2.4 ferric uptake regulator (NCBI) compare
b0741 pal -5.8 -4.0 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b1850 eda -5.7 -2.7 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b1949 fliQ -5.6 -3.0 flagellar biosynthesis protein Q (NCBI) compare
b1882 cheY -5.6 -1.3 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b0737 tolQ -5.4 -3.8 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0014 dnaK -5.2 -2.8 molecular chaperone DnaK (NCBI) compare
b3390 aroK -5.0 -2.9 shikimate kinase I (NCBI) compare
b0222 lpcA -4.7 -5.2 phosphoheptose isomerase (NCBI) compare
b2846 yqeH -4.7 -2.5 orf, hypothetical protein (VIMSS) compare
b3463 ftsE -4.7 -7.8 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b4704 arrS -4.6 -1.6 Antisense sRNA ArrS, function unknown (NCBI) compare
b0688 pgm -4.5 -5.3 phosphoglucomutase (NCBI) compare
b1286 rnb -4.5 -5.5 exoribonuclease II (NCBI) compare
b3164 pnp -4.4 -4.0 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b3398 yrfF -4.4 -4.8 predicted inner membrane protein (NCBI) compare
b1499 ydeO -4.3 -2.7 predicted DNA-binding transcriptional acfivator (NCBI) compare
b2567 rnc -4.3 -2.3 ribonuclease III (NCBI) compare
b0739 tolA -4.3 -7.1 cell envelope integrity inner membrane protein TolA (NCBI) compare
b3778 rep -4.2 -12.5 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b3500 gor -4.2 -2.7 glutathione reductase (NCBI) compare
b0439 lon -4.2 -6.4 DNA-binding ATP-dependent protease La (NCBI) compare
b3725 pstB -4.2 -2.9 phosphate transporter subunit (NCBI) compare
b1861 ruvA -4.1 -2.6 Holliday junction DNA helicase motor protein (NCBI) compare
b3636 rpmG -4.1 -2.3 50S ribosomal protein L33 (NCBI) compare
b3183 obgE -4.1 -2.0 GTPase involved in cell partioning and DNA repair (NCBI) compare
b1942 fliJ -4.1 -1.4 flagellar biosynthesis chaperone (NCBI) compare
b4375 prfC -4.1 -6.8 peptide chain release factor 3 (NCBI) compare
b4239 treC -4.0 -7.7 trehalose-6-P hydrolase (NCBI) compare
b3619 rfaD -4.0 -7.6 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b2935 tktA -3.9 -3.8 transketolase 1, thiamin-binding (NCBI) compare
b2288 nuoA -3.9 -4.4 NADH dehydrogenase I chain A (VIMSS) compare
b1860 ruvB -3.9 -2.1 Holliday junction DNA helicase B (NCBI) compare
b0500 ybbD -3.9 -2.3 hypothetical protein (RefSeq) compare
b1293 sapB -3.9 -2.4 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0564 appY -3.9 -1.3 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b3026 qseC -3.9 -8.6 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b0623 cspE -3.8 -1.8 cold shock protein E (NCBI) compare
b2330 yfcB -3.8 -2.4 putative adenine-specific methylase (VIMSS) compare
b3052 rfaE -3.8 -8.5 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3609 secB -3.8 -5.6 export protein SecB (NCBI) compare
b0605 ahpC -3.8 -6.1 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b3790 rffC -3.8 -6.7 TDP-fucosamine acetyltransferase (RefSeq) compare
b4651 yibW -3.8 -2.4 no description compare
b3386 rpe -3.7 -2.6 ribulose-phosphate 3-epimerase (NCBI) compare
b2699 recA -3.6 -2.5 recombinase A (NCBI) compare
b1712 ihfA -3.6 -3.7 integration host factor subunit alpha (NCBI) compare
b2314 dedD -3.5 -3.9 putative lipoprotein (VIMSS) compare
b2576 srmB -3.5 -5.4 ATP-dependent RNA helicase (NCBI) compare
b1236 galU -3.5 -7.3 glucose-1-phosphate uridylyltransferase (NCBI) compare
b1950 fliR -3.5 -5.8 flagellar biosynthesis protein R (NCBI) conserved
b0888 trxB -3.5 -4.3 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b4177 purA -3.4 -5.3 adenylosuccinate synthetase (NCBI) compare
b2830 nudH -3.4 -2.5 dinucleoside polyphosphate hydrolase (NCBI) compare
b2110 yehC -3.4 -3.9 predicted periplasmic pilin chaperone (NCBI) compare
b3792 wzxE -3.4 -7.6 O-antigen translocase (NCBI) compare
b0740 tolB -3.4 -6.5 translocation protein TolB precursor (NCBI) compare
b4443 gcvB -3.3 -1.6 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b3839 tatC -3.3 -9.4 TatABCE protein translocation system subunit (NCBI) compare
b2175 spr -3.3 -3.9 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b1167 ymgC -3.2 -1.9 hypothetical protein (NCBI) compare
b3631 rfaG -3.2 -17.1 glucosyltransferase I (NCBI) compare
b3630 rfaP -3.2 -14.1 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b3462 ftsX -3.2 -8.4 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1863 ruvC -3.2 -4.0 Holliday junction resolvase (NCBI) compare
b3620 rfaF -3.2 -7.7 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b4202 rpsR -3.1 -2.7 30S ribosomal protein S18 (NCBI) compare
b0890 ftsK -3.1 -11.2 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b0603 ybdO -3.1 -3.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b0080 fruR -3.1 -7.2 DNA-binding transcriptional dual regulator (NCBI) compare
b2569 lepA -3.1 -6.1 GTP-binding protein LepA (NCBI) compare
b2620 smpB -3.0 -2.3 SsrA-binding protein (NCBI) compare
b3977 tyrU -3.0 -1.0 tRNA-Tyr (NCBI) compare
b0200 gmhB -3.0 -2.9 hypothetical protein (NCBI) compare
b0082 mraW -3.0 -4.3 S-adenosyl-methyltransferase (NCBI) compare
b4481 rffT -3.0 -7.6 4-alpha-L-fucosyltransferase (NCBI) compare
b3842 rfaH -3.0 -5.6 transcriptional activator RfaH (NCBI) compare
b2820 recB -3.0 -4.5 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b2894 xerD -2.9 -1.9 site-specific tyrosine recombinase XerD (NCBI) compare
b1938 fliF -2.9 -6.5 flagellar M-ring protein (NCBI) conserved
b2635 ypjK -2.9 -2.7 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b4172 hfq -2.8 -2.4 RNA-binding protein Hfq (NCBI) compare
b2808 gcvA -2.8 -4.2 DNA-binding transcriptional dual regulator (NCBI) compare
b0118 acnB -2.8 -3.3 aconitate hydratase (NCBI) compare
b1257 yciE -2.8 -3.0 hypothetical protein (NCBI) compare
b1081 flgJ -2.7 -4.7 flagellar biosynthesis protein FlgJ (NCBI) compare
b4201 priB -2.7 -3.1 primosomal replication protein N (NCBI) compare
b1175 minD -2.7 -5.5 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b3387 dam -2.7 -10.1 DNA adenine methylase (NCBI) compare
b1334 fnr -2.7 -2.9 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b3613 yibP -2.7 -5.5 putative membrane protein (VIMSS) compare
b3871 bipA -2.7 -8.0 GTP-binding protein (NCBI) compare
b4577 sgrS -2.7 -1.3 small antisense RNA (NCBI) compare
b1884 cheR -2.7 -4.1 chemotaxis regulator, protein-glutamate methyltransferase (NCBI) compare
b0406 tgt -2.7 -5.2 queuine tRNA-ribosyltransferase (NCBI) compare
b3791 rffA -2.7 -5.8 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b0018 mokC -2.7 -0.9 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b3261 fis -2.6 -3.7 DNA-binding protein Fis (NCBI) compare
b3806 cyaA -2.6 -8.4 adenylate cyclase (NCBI) compare
b3162 deaD -2.6 -6.0 inducible ATP-independent RNA helicase (VIMSS) compare
b2951 yggS -2.6 -3.0 predicted enzyme (NCBI) compare
b3027 ygiZ -2.6 -1.7 conserved inner membrane protein (NCBI) compare
b3811 xerC -2.6 -4.4 site-specific tyrosine recombinase XerC (NCBI) compare
b0949 uup -2.6 -6.9 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b3741 gidA -2.5 -7.9 glucose-inhibited division protein A (NCBI) compare
b3621 rfaC -2.5 -6.7 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b1940 fliH -2.5 -3.2 flagellar biosynthesis; export of flagellar proteins? (VIMSS) compare
b3504 yhiS -2.5 -3.1 hypothetical protein (RefSeq) compare
b1434 ydcN -2.5 -1.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b1943 fliK -2.5 -4.1 flagellar hook-length control protein (NCBI) compare
b0405 queA -2.4 -3.8 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI) compare
b1548 nohA -2.4 -1.7 Qin prophage; predicted packaging protein (RefSeq) compare
b4042 dgkA -2.4 -3.5 diacylglycerol kinase (NCBI) compare
b0051 ksgA -2.4 -4.8 dimethyladenosine transferase (NCBI) compare
b1497 b1497 -2.4 -3.8 putative enzyme (VIMSS) compare
b3442 yhhZ -2.4 -2.7 hypothetical protein (NCBI) compare
b1058 yceO -2.4 -1.4 hypothetical protein (NCBI) compare
b3002 yqhA -2.4 -1.7 hypothetical protein (NCBI) compare
b3290 trkA -2.4 -4.5 potassium transporter peripheral membrane component (NCBI) compare
b1073 flgB -2.4 -2.7 flagellar basal-body rod protein B (NCBI) compare
b1182 hlyE -2.3 -2.2 hemolysin E (VIMSS) compare
b2058 wcaB -2.3 -1.0 predicted acyl transferase (NCBI) compare
b2847 yqeI -2.3 -5.5 predicted transcriptional regulator (NCBI) compare
b0053 surA -2.3 -6.2 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b3781 trxA -2.3 -2.3 thioredoxin 1 (VIMSS) compare
b3205 yhbJ -2.2 -3.6 hypothetical protein (NCBI) compare
b0866 ybjQ -2.2 -0.9 hypothetical protein (NCBI) compare
b4000 hupA -2.2 -2.5 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b3933 ftsN -2.2 -6.2 essential cell division protein (NCBI) compare
b0015 dnaJ -2.2 -4.9 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b4458 oxyS -2.2 -0.9 global regulatory RNA (NCBI) compare
b1922 fliA -2.2 -3.6 flagellar biosynthesis; alternative sigma factor 28; regulation of flagellar operons (VIMSS) compare
b3706 trmE -2.2 -4.8 tRNA modification GTPase (NCBI) compare
b2809 b2809 -2.2 -1.6 orf, hypothetical protein (VIMSS) compare
b0843 ybjH -2.2 -1.7 hypothetical protein (NCBI) compare
b4497 yeeL -2.1 -4.5 no description compare
b1545 pinQ -2.1 -1.0 Qin prophage; predicted site-specific recombinase (NCBI) compare
b4402 yjjY -2.1 -1.2 hypothetical protein (NCBI) compare
b1831 proQ -2.1 -2.0 putative solute/DNA competence effector (NCBI) compare
b0738 tolR -2.1 -3.1 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3838 tatB -2.1 -5.2 sec-independent translocase (NCBI) compare
b1049 mdoH -2.1 -6.1 glucosyltransferase MdoH (NCBI) compare
b1830 prc -2.1 -4.5 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b1904 yecR -2.1 -2.2 hypothetical protein (NCBI) compare
b0550 rusA -2.1 -2.3 DLP12 prophage; endonuclease RUS (NCBI) compare
b2501 ppk -2.1 -6.3 polyphosphate kinase (NCBI) compare
b3728 pstS -2.1 -6.0 phosphate transporter subunit (NCBI) compare
b2614 grpE -2.0 -1.7 heat shock protein (NCBI) compare
b1079 flgH -2.0 -2.2 flagellar L-ring protein precursor H (NCBI) compare
b1946 fliN -2.0 -2.1 flagellar motor switch protein (NCBI) compare
b1505 ydeT -2.0 -4.3 hypothetical protein (RefSeq) compare
b2910 zapA -2.0 -2.9 protein that localizes to the cytokinetic ring (NCBI) compare
b4355 tsr -2.0 -6.7 methyl-accepting chemotaxis protein I, serine sensor receptor (NCBI) compare
b2947 gshB -2.0 -3.9 glutathione synthetase (NCBI) compare
b3009 yghB -2.0 -4.1 conserved inner membrane protein (NCBI) compare
b4203 rplI -2.0 -3.2 50S ribosomal protein L9 (NCBI) compare
b3979 thrT -2.0 -0.8 tRNA-Thr (NCBI) compare
b3035 tolC -2.0 -4.3 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b1890 motA -2.0 -1.9 flagellar motor protein MotA (NCBI) compare
b1941 fliI -2.0 -3.8 flagellum-specific ATP synthase (NCBI) conserved
b3786 rffE -2.0 -8.9 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b1080 flgI -2.0 -3.6 homolog of Salmonella P-ring of flagella basal body (VIMSS) conserved
b1880 flhB -2.0 -2.9 flagellar biosynthesis protein B (NCBI) conserved
b1945 fliM -2.0 -3.2 flagellar motor switch protein M (NCBI) conserved
b2822 recC -2.0 -5.5 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b1865 nudB -1.9 -1.5 dATP pyrophosphohydrolase (NCBI) compare
b2282 nuoH -1.9 -2.6 NADH dehydrogenase subunit H (NCBI) compare
b3389 aroB -1.9 -5.8 3-dehydroquinate synthase (NCBI) compare
b0232 yafN -1.9 -1.0 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b1886 tar -1.9 -4.0 methyl-accepting chemotaxis protein II (NCBI) compare
b1078 flgG -1.9 -3.1 flagellar component of cell-distal portion of basal-body rod (NCBI) compare
b4153 frdB -1.9 -5.2 fumarate reductase (anaerobic), Fe-S subunit (NCBI) compare
b3787 rffD -1.9 -7.2 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b0907 serC -1.9 -4.1 phosphoserine aminotransferase (NCBI) compare
b4585 sroB -1.9 -0.8 Novel sRNA, function unknown (RefSeq) compare
b3169 nusA -1.9 -1.9 transcription elongation factor NusA (NCBI) compare
b3860 dsbA -1.9 -7.0 periplasmic protein disulfide isomerase I (NCBI) compare
b2897 ygfY -1.9 -2.8 hypothetical protein (NCBI) compare
b1363 trkG -1.9 -2.9 Rac prophage; potassium transporter subunit (NCBI) compare
b1925 fliS -1.8 -3.0 flagellar protein FliS (NCBI) conserved
b3206 npr -1.8 -3.0 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b4590 ybfK -1.8 -2.8 hypothetical protein (RefSeq) compare
b1887 cheW -1.8 -3.2 purine-binding chemotaxis protein (NCBI) compare
b1001 yccE -1.8 -1.8 hypothetical protein (NCBI) compare
b1888 cheA -1.8 -3.0 fused chemotactic sensory histidine kinase in two-component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein (NCBI) compare
b1095 fabF -1.8 -4.3 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b1252 tonB -1.8 -3.0 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b4441 glmY -1.8 -1.0 sRNA activator of glmS mRNA (RefSeq) compare
b1176 minC -1.8 -1.9 septum formation inhibitor (NCBI) compare
b1883 cheB -1.8 -1.6 chemotaxis-specific methylesterase (NCBI) compare
b1804 rnd -1.7 -2.9 ribonuclease D (NCBI) compare
b2262 menB -1.7 -2.9 naphthoate synthase (NCBI) compare
b2421 cysM -1.7 -3.0 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b1677 lpp -1.7 -1.9 murein lipoprotein (NCBI) compare
b1939 fliG -1.7 -1.6 flagellar motor switch protein G (NCBI) compare
b4128 yjdK -1.7 -2.4 hypothetical protein (NCBI) compare
b1077 flgF -1.7 -3.5 flagellar component of cell-proximal portion of basal-body rod (NCBI) compare
b1693 aroD -1.7 -3.9 3-dehydroquinate dehydratase (NCBI) compare
b3348 slyX -1.7 -2.8 hypothetical protein (NCBI) compare
b0720 gltA -1.7 -4.4 citrate synthase (NCBI) compare


Specific Phenotypes

For 18 genes in this experiment

For motility Agar in Escherichia coli BW25113

For motility Agar across organisms