Experiment set6IT064 for Pseudomonas putida KT2440

Valerolactam carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + 2-Piperidinone (10 mM)
Culturing: Putida_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 5.3 generations
By: Mitch on 12/8/17
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 3 genes in this experiment

For carbon source 2-Piperidinone in Pseudomonas putida KT2440

For carbon source 2-Piperidinone across organisms

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Urea cycle and metabolism of amino groups
• Glutamate metabolism
• Geraniol degradation
• Lysine biosynthesis
• Lysine degradation
• Arginine and proline metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• 1- and 2-Methylnaphthalene degradation
• Naphthalene and anthracene degradation
• Glyoxylate and dicarboxylate metabolism
• Butanoate metabolism
• Limonene and pinene degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glutarate degradation	2	2	2
4-aminobutanoate degradation III	2	2	1
L-lysine degradation I	7	5	2
L-lysine degradation IV	5	5	1
L-lysine degradation X	6	5	1
L-lysine degradation III	6	2	1
photorespiration I	9	6	1
superpathway of L-lysine degradation	43	23	2