Experiment set6IT058 for Pseudomonas putida KT2440
Sodium acetate carbon source
Group: carbon sourceMedia: MOPS minimal media_noCarbon + Sodium acetate (10 mM)
Culturing: Putida_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 4.2 generations
By: Mitch on 12/8/17
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 3 genes in this experiment
For carbon source Sodium acetate in Pseudomonas putida KT2440
For carbon source Sodium acetate across organisms
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| acetate formation from acetyl-CoA (succinate) | 1 | 1 | 1 |
| pyruvate fermentation to acetate V | 3 | 3 | 1 |
| pyruvate fermentation to acetate VI | 3 | 2 | 1 |
| succinate fermentation to butanoate | 7 | 1 | 1 |
| TCA cycle VII (acetate-producers) | 9 | 8 | 1 |
| anaerobic energy metabolism (invertebrates, mitochondrial) | 10 | 5 | 1 |
| superpathway of anaerobic energy metabolism (invertebrates) | 17 | 10 | 1 |