Experiment set6IT054 for Escherichia coli BW25113

Compare to:

LB with D-Cycloserine 0.1 mg/ml

Group: stress
Media: LB + D-Cycloserine (0.1 mg/ml)
Culturing: Keio_ML9, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 2/2/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1134 C7,C8

Specific Phenotypes

For 8 genes in this experiment

For stress D-Cycloserine in Escherichia coli BW25113

For stress D-Cycloserine across organisms

SEED Subsystems

Subsystem #Specific
Alanine biosynthesis 1
DNA repair, bacterial 1
Oxidative stress 1
Pyruvate Alanine Serine Interconversions 1
Transport of Manganese 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-alanine biosynthesis II 1 1 1
L-alanine degradation III 1 1 1
alanine racemization 1 1 1
superpathway of L-alanine biosynthesis 4 4 2
L-alanine degradation V (oxidative Stickland reaction) 2 2 1
L-alanine degradation I 2 2 1
L-alanine biosynthesis I 2 2 1
L-alanine degradation II (to D-lactate) 3 2 1
pyruvate fermentation to acetate and alanine 3 2 1
anaerobic energy metabolism (invertebrates, cytosol) 7 5 2
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 8 2
L-alanine degradation VI (reductive Stickland reaction) 6 2 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 9 2
UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) 8 8 1
UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) 8 7 1
UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) 8 6 1
superpathway of anaerobic energy metabolism (invertebrates) 17 12 2
superpathway of L-alanine fermentation (Stickland reaction) 9 5 1
ansatrienin biosynthesis 9 1 1
peptidoglycan biosynthesis I (meso-diaminopimelate containing) 12 12 1
peptidoglycan biosynthesis III (mycobacteria) 15 11 1
cyclosporin A biosynthesis 15 2 1
peptidoglycan biosynthesis II (staphylococci) 17 12 1
peptidoglycan biosynthesis IV (Enterococcus faecium) 17 12 1
peptidoglycan biosynthesis V (β-lactam resistance) 17 11 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 22 1