Experiment set69S550 for Escherichia coli BW25113
RB68
Group: phageMedia: LB_plus_SM_buffer + MOI 10
Culturing: Keio_ML9a, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 12/4/24
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 13 genes in this experiment
For phage MOI 10 in Escherichia coli BW25113
For phage MOI 10 across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycine, serine and threonine metabolism
- Benzoate degradation via CoA ligation
- Butanoate metabolism
- Fructose and mannose metabolism
- Ascorbate and aldarate metabolism
- C21-Steroid hormone metabolism
- Pyrimidine metabolism
- Lysine biosynthesis
- Bisphenol A degradation
- Nucleotide sugars metabolism
- Polyketide sugar unit biosynthesis
- Aminosugars metabolism
- Linoleic acid metabolism
- Tetrachloroethene degradation
- Retinol metabolism
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of type II polyketide products
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-threonine degradation II | 2 | 2 | 1 |
| pseudouridine degradation | 2 | 2 | 1 |
| D-xylose degradation to ethylene glycol (engineered) | 4 | 3 | 1 |
| dipicolinate biosynthesis | 4 | 3 | 1 |
| adipate degradation | 5 | 5 | 1 |
| adipate biosynthesis | 5 | 4 | 1 |
| L-lysine biosynthesis III | 7 | 6 | 1 |
| L-lysine biosynthesis VI | 7 | 6 | 1 |
| D-xylose degradation IV | 7 | 3 | 1 |
| benzoyl-CoA degradation I (aerobic) | 7 | 3 | 1 |
| L-lysine biosynthesis I | 9 | 9 | 1 |
| phenylacetate degradation I (aerobic) | 9 | 9 | 1 |
| Entner-Doudoroff pathway II (non-phosphorylative) | 9 | 6 | 1 |
| L-lysine biosynthesis II | 9 | 6 | 1 |
| superpathway of phenylethylamine degradation | 11 | 11 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis II | 15 | 13 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis I | 18 | 18 | 1 |
| superpathway of L-threonine metabolism | 18 | 16 | 1 |
| aspartate superpathway | 25 | 24 | 1 |
| superpathway of pentose and pentitol degradation | 42 | 18 | 1 |