Experiment set69S549 for Escherichia coli BW25113
RB68
Group: phageMedia: LB_plus_SM_buffer + MOI 10
Culturing: Keio_ML9a, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 12/4/24
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 17 genes in this experiment
For phage MOI 10 in Escherichia coli BW25113
For phage MOI 10 across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| LOS core oligosaccharide biosynthesis | 1 |
| Molybdenum cofactor biosynthesis | 1 |
| Nitrate and nitrite ammonification | 1 |
| Oxidative stress | 1 |
| Transport of Molybdenum | 1 |
| tRNA processing | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Nucleotide sugars metabolism
- Benzoate degradation via CoA ligation
- Butanoate metabolism
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
- Pentose phosphate pathway
- Fructose and mannose metabolism
- Ascorbate and aldarate metabolism
- Ubiquinone and menaquinone biosynthesis
- C21-Steroid hormone metabolism
- Puromycin biosynthesis
- Pyrimidine metabolism
- Glycine, serine and threonine metabolism
- Bisphenol A degradation
- Polyketide sugar unit biosynthesis
- Aminosugars metabolism
- Lipopolysaccharide biosynthesis
- Linoleic acid metabolism
- alpha-Linolenic acid metabolism
- Tetrachloroethene degradation
- Trinitrotoluene degradation
- Nicotinate and nicotinamide metabolism
- Retinol metabolism
- Porphyrin and chlorophyll metabolism
- Carotenoid biosynthesis - General
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of type II polyketide products
- Biosynthesis of alkaloids derived from shikimate pathway
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: