Experiment set68S105 for Escherichia coli BW25113
HMW_DNA_from_Pseudomonas_EB271-B4-1 46.9 ppm carbon source
Group: carbon sourceMedia: Chakraborty_Basal + HMW_DNA_from_Pseudomonas_EB271-B4-1 (46.9 ppm), pH=7
Culturing: Keio_ML9a, tube, Aerobic, at 29 (C), shaken=200 rpm
By: Bri Finley on 9/16/24
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 0.3792 g/L Sodium phosphate monobasic, 0.9713 g/L Disodium phosphate, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 3 genes in this experiment
For carbon source HMW_DNA_from_Pseudomonas_EB271-B4-1 in Escherichia coli BW25113
For carbon source HMW_DNA_from_Pseudomonas_EB271-B4-1 across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glycerol degradation I | 3 | 3 | 2 |
| glycerol and glycerophosphodiester degradation | 4 | 4 | 2 |
| arsenate detoxification III | 2 | 2 | 1 |
| ribose phosphorylation | 2 | 2 | 1 |
| 2-deoxy-D-ribose degradation I | 3 | 3 | 1 |
| arsenic detoxification (plants) | 6 | 5 | 1 |
| arsenic detoxification (yeast) | 12 | 4 | 1 |
| arsenic detoxification (mammals) | 17 | 8 | 1 |