Experiment set67S62 for Escherichia coli BW25113

Compare to:

Cytidine 0.41115 mM carbon source

200 most detrimental genes:

  gene name fitness t score description  
b0142 folK +2.0 2.1 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b2903 gcvP +1.6 14.2 glycine dehydrogenase (NCBI) compare
b0232 yafN +1.5 2.1 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b2904 gcvH +1.4 7.8 glycine cleavage system protein H (NCBI) compare
b4552 yrhC +1.3 1.9 no description compare
b2905 gcvT +1.3 9.8 glycine cleavage system aminomethyltransferase T (NCBI) compare
b3933 ftsN +1.2 8.7 essential cell division protein (NCBI) compare
b3979 thrT +1.1 0.6 tRNA-Thr (NCBI) compare
b4701 sokX +1.1 1.8 sok-related sRNA, function unknown (RefSeq) compare
b2808 gcvA +1.0 4.5 DNA-binding transcriptional dual regulator (NCBI) compare
b2953 yggU +0.9 2.3 orf, hypothetical protein (VIMSS) compare
b1961 dcm +0.9 2.1 DNA cytosine methylase (NCBI) compare
b4503 yafF +0.8 0.9 no description compare
b1685 ydiH +0.8 2.0 predicted protein (RefSeq) compare
b4520 ymgF +0.8 2.5 hypothetical protein (NCBI) compare
b1038 csgF +0.8 2.9 predicted transport protein (NCBI) compare
b2688 gshA +0.7 4.8 glutamate--cysteine ligase (NCBI) compare
b4699 fnrS +0.7 1.1 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1651 gloA +0.7 2.5 glyoxalase I, Ni-dependent (NCBI) compare
b2947 gshB +0.7 4.2 glutathione synthetase (NCBI) compare
b3194 yrbE +0.7 4.2 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1037 csgG +0.7 5.4 outer membrane lipoprotein (NCBI) compare
b1824 yobF +0.6 2.0 hypothetical protein (NCBI) compare
b0632 dacA +0.6 4.6 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b2343 b2343 +0.6 2.1 orf, hypothetical protein (VIMSS) compare
b4418 sraB +0.6 2.2 unknown RNA (NCBI) compare
b1733 chbG +0.6 3.7 hypothetical protein (NCBI) compare
b1125 potB +0.6 2.6 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b1942 fliJ +0.6 0.9 flagellar biosynthesis chaperone (NCBI) compare
b2939 yqgB +0.6 0.9 predicted protein (RefSeq) compare
b1849 purT +0.6 2.2 phosphoribosylglycinamide formyltransferase 2 (NCBI) compare
b4603 rseX +0.6 0.8 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b4679 yohP +0.6 1.6 expressed protein (RefSeq) compare
b1144 ymfJ +0.6 2.5 orf, hypothetical protein (VIMSS) compare
b2314 dedD +0.6 3.4 putative lipoprotein (VIMSS) compare
b2168 fruK +0.6 1.1 1-phosphofructokinase (NCBI) compare
b0059 hepA +0.6 5.0 ATP-dependent helicase HepA (NCBI) compare
b2572 rseA +0.6 3.0 anti-sigma factor (NCBI) compare
b4588 ylcH +0.6 2.1 hypothetical protein, DLP12 prophage (RefSeq) compare
b1524 yneH +0.5 1.0 predicted glutaminase (NCBI) compare
b4592 yccB +0.5 0.8 hypothetical protein (RefSeq) compare
b2145 yeiS +0.5 2.2 predicted inner membrane protein (NCBI) compare
b1333 uspE +0.5 1.9 stress-induced protein (NCBI) compare
b2114 metG +0.5 1.8 methionyl-tRNA synthetase (NCBI) compare
b1514 lsrC +0.5 2.1 AI2 transporter (NCBI) compare
b1730 ydjO +0.5 1.1 predicted protein (RefSeq) compare
b0149 mrcB +0.5 4.3 penicillin-binding protein 1b (NCBI) compare
b1295 ymjA +0.5 1.6 hypothetical protein (NCBI) compare
b4175 hflC +0.5 3.3 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b1103 ycfF +0.5 0.9 orf, hypothetical protein (VIMSS) compare
b1792 yeaO +0.5 2.1 orf, hypothetical protein (VIMSS) compare
b4505 insX +0.5 1.3 predicted IS protein (RefSeq) compare
b1672 ydhW +0.5 1.7 hypothetical protein (NCBI) compare
b0802 ybiJ +0.5 1.3 hypothetical protein (NCBI) compare
b1041 csgB +0.5 2.2 curlin nucleator protein, minor subunit in curli complex (NCBI) compare
b1382 ynbE +0.5 1.1 predicted lipoprotein (NCBI) compare
b1633 nth +0.5 2.2 DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) (NCBI) compare
b3181 greA +0.5 2.8 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b2699 recA +0.5 0.7 recombinase A (NCBI) compare
b1287 yciW +0.5 2.7 putative oxidoreductase (VIMSS) compare
b4260 pepA +0.5 3.7 leucyl aminopeptidase (NCBI) compare
b1187 fadR +0.5 2.0 fatty acid metabolism regulator (NCBI) compare
b2597 yfiA +0.5 1.4 cold shock protein associated with 30S ribosomal subunit (NCBI) compare
b2579 yfiD +0.4 1.1 pyruvate formate lyase subunit (NCBI) compare
b4203 rplI +0.4 2.0 50S ribosomal protein L9 (NCBI) compare
b4441 glmY +0.4 1.3 sRNA activator of glmS mRNA (RefSeq) compare
b3769 ilvM +0.4 1.8 acetolactate synthase II, small subunit (NCBI) compare
b4538 yoeF +0.4 1.3 hypothetical protein (RefSeq) compare
b1936 intG +0.4 0.5 predicted defective phage integrase (pseudogene) (RefSeq) compare
b0505 allA +0.4 2.0 ureidoglycolate hydrolase (NCBI) compare
b0852 rimK +0.4 2.8 ribosomal protein S6 modification protein (NCBI) compare
b4110 yjcZ +0.4 3.3 orf, hypothetical protein (VIMSS) compare
b2743 pcm +0.4 2.1 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b4692 oweE +0.4 1.4 no description compare
b3775 ppiC +0.4 2.4 peptidyl-prolyl cis-trans isomerase C (rotamase C) (NCBI) compare
b1486 ddpB +0.4 1.7 D-ala-D-ala transporter subunit (NCBI) compare
b1727 yniC +0.4 1.8 predicted hydrolase (NCBI) compare
b2421 cysM +0.4 1.9 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b0921 smtA +0.4 2.8 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b2261 menC +0.4 2.6 O-succinylbenzoate synthase (NCBI) compare
b2309 hisJ +0.4 2.3 histidine/lysine/arginine/ornithine transporter subunit (NCBI) compare
b1159 mcrA +0.4 0.7 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b1586 b1586 +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b1863 ruvC +0.4 1.3 Holliday junction resolvase (NCBI) compare
b0685 ybfE +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b4217 ytfK +0.4 2.2 orf, hypothetical protein (VIMSS) compare
b1520 b1520 +0.4 1.1 orf, hypothetical protein (VIMSS) compare
b4654 yjdQ +0.4 3.1 no description compare
b3934 cytR +0.4 3.4 DNA-binding transcriptional dual regulator (NCBI) compare
b2352 yfdI +0.4 0.5 CPS-53 (KpLE1) prophage; predicted inner membrane protein (NCBI) compare
b2765 ygcM +0.4 1.6 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) (NCBI) compare
b0796 ybiH +0.4 1.6 putative transcriptional regulator (VIMSS) compare
b3293 yhdN +0.4 1.6 hypothetical protein (NCBI) compare
b1754 b1754 +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b3263 yhdU +0.4 2.4 predicted membrane protein (NCBI) compare
b1519 tam +0.4 2.4 trans-aconitate 2-methyltransferase (NCBI) compare
b0461 ybaJ +0.4 2.6 hypothetical protein (NCBI) compare
b2141 yohJ +0.4 1.3 hypothetical protein (NCBI) compare
b2768 ygcP +0.4 1.2 predicted anti-terminator regulatory protein (NCBI) compare
b2007 yeeX +0.4 0.9 conserved protein (RefSeq) compare
b4416 rybA +0.4 0.9 unknown RNA (NCBI) compare
b2446 yffO +0.4 1.2 CPZ-55 prophage; predicted protein (NCBI) compare
b0247 ykfG +0.4 0.9 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b2564 pdxJ +0.4 1.8 pyridoxal phosphate biosynthetic protein (NCBI) compare
b1205 ychH +0.4 1.4 predicted inner membrane protein (NCBI) compare
b1944 fliL +0.4 2.0 flagellar basal body-associated protein FliL (NCBI) compare
b1233 ychJ +0.4 1.3 hypothetical protein (NCBI) compare
b1871 yecP +0.4 1.7 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b4255 yjgD +0.4 2.2 hypothetical protein (NCBI) compare
b2125 yehT +0.4 1.6 orf, hypothetical protein (VIMSS) compare
b1920 fliY +0.4 0.8 cystine transporter subunit (NCBI) compare
b2534 yfhR +0.4 2.4 predicted peptidase (RefSeq) compare
b1396 paaI +0.4 2.4 predicted thioesterase (NCBI) compare
b1724 ydiZ +0.4 1.3 hypothetical protein (NCBI) compare
b4630 ykgP +0.4 1.6 no description compare
b3098 yqjD +0.4 2.0 hypothetical protein (NCBI) compare
b4594 ymgJ +0.4 1.1 hypothetical protein (RefSeq) compare
b0527 ybcI +0.4 1.8 conserved inner membrane protein (NCBI) compare
b2481 hyfA +0.4 2.1 hydrogenase 4 Fe-S subunit (VIMSS) compare
b1823 cspC +0.4 0.9 stress protein, member of the CspA-family (NCBI) compare
b1526 yneJ +0.4 1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b2362 yfdS +0.4 2.2 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b3935 priA +0.4 1.0 primosome assembly protein PriA (NCBI) compare
b1611 fumC +0.4 1.5 fumarate hydratase (NCBI) compare
b1279 yciS +0.4 0.6 conserved inner membrane protein (NCBI) compare
b1806 yeaY +0.3 1.8 predicted lipoprotein (NCBI) compare
b2637 yfjT +0.3 1.2 CP4-57 prophage; predicted protein (NCBI) compare
b3484 yhhI +0.3 1.1 predicted transposase (NCBI) compare
b2293 yfbT +0.3 1.2 putative phosphatase (VIMSS) compare
b1042 csgA +0.3 2.2 cryptic curlin major subunit (NCBI) compare
b0785 moaE +0.3 2.0 molybdopterin synthase, large subunit (NCBI) compare
b1273 yciN +0.3 0.8 hypothetical protein (NCBI) compare
b4536 yobH +0.3 1.2 hypothetical protein (NCBI) compare
b2832 ygdQ +0.3 2.4 predicted inner membrane protein (NCBI) compare
b3199 yrbK +0.3 1.4 hypothetical protein (NCBI) compare
b1079 flgH +0.3 1.7 flagellar L-ring protein precursor H (NCBI) compare
b0487 cueR +0.3 1.0 DNA-binding transcriptional activator of copper-responsive regulon genes (NCBI) compare
b4585 sroB +0.3 0.6 Novel sRNA, function unknown (RefSeq) compare
b1482 osmC +0.3 0.7 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b0365 tauA +0.3 2.0 taurine transport system periplasmic protein (VIMSS) compare
b1186 nhaB +0.3 2.2 NhaB sodium/proton transporter (Katherine Huang) compare
b3034 nudF +0.3 2.2 ADP-ribose pyrophosphatase (NCBI) compare
b1696 ydiP +0.3 0.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b1077 flgF +0.3 2.1 flagellar component of cell-proximal portion of basal-body rod (NCBI) compare
b0754 aroG +0.3 1.9 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, phenylalanine repressible (NCBI) compare
b1777 b1777 +0.3 0.9 orf, hypothetical protein (VIMSS) compare
b1190 dadX +0.3 1.8 alanine racemase (NCBI) compare
b0979 appB +0.3 2.1 cytochrome bd-II oxidase, subunit II (NCBI) compare
b0729 sucD +0.3 0.9 succinyl-CoA synthetase subunit alpha (NCBI) compare
b1518 lsrG +0.3 1.0 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b4656 yjhY +0.3 2.0 no description compare
b0742 ybgF +0.3 1.9 hypothetical protein (NCBI) compare
b4513 kdpF +0.3 1.1 potassium ion accessory transporter subunit (NCBI) compare
b4371 rsmC +0.3 2.2 16S ribosomal RNA m2G1207 methyltransferase (NCBI) compare
b1278 pgpB +0.3 1.3 phosphatidylglycerophosphatase B (NCBI) compare
b4141 yjeH +0.3 2.6 predicted transporter (NCBI) compare
b1183 umuD +0.3 0.6 DNA polymerase V, subunit D (NCBI) compare
b0618 citC +0.3 2.0 citrate lyase synthetase (citrate (pro-3S)-lyase ligase (VIMSS) compare
b1960 vsr +0.3 0.9 DNA mismatch endonuclease of very short patch repair (NCBI) compare
b1948 fliP +0.3 2.0 flagellar biosynthesis protein P (NCBI) compare
b1299 puuR +0.3 1.2 DNA-binding transcriptional repressor (NCBI) compare
b1213 ychQ +0.3 1.9 predicted transcriptional regulator (NCBI) compare
b2774 ygcW +0.3 1.4 putative oxidoreductase (VIMSS) compare
b1988 nac +0.3 1.6 DNA-binding transcriptional dual regulator of nitrogen assimilation (NCBI) compare
b1120 cobB +0.3 1.7 deacetylase of acs and cheY, regulates chemotaxis (NCBI) compare
b4212 ytfH +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b2246 b2246 +0.3 1.8 putative transport protein (VIMSS) compare
b0357 yaiN +0.3 0.6 putative alpha helix chain (VIMSS) compare
b3446 yrhB +0.3 1.8 hypothetical protein (NCBI) compare
b4370 leuQ +0.3 1.0 tRNA-Leu (NCBI) compare
b2934 cmtB +0.3 0.4 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b2325 yfcL +0.3 1.8 hypothetical protein (NCBI) compare
b4406 yaeP +0.3 1.2 hypothetical protein (NCBI) compare
b0643 ybeL +0.3 1.5 hypothetical protein (NCBI) compare
b1412 azoR +0.3 1.4 acyl carrier protein phosphodiesterase (NCBI) compare
b2458 eutI +0.3 1.6 predicted phosphotransacetylase subunit (NCBI) compare
b4373 rimI +0.3 1.5 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b1283 osmB +0.3 1.9 lipoprotein (NCBI) compare
b4457 csrC +0.3 1.4 regulatory RNA (NCBI) compare
b4174 hflK +0.3 2.2 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b2521 sseA +0.3 2.5 putative thiosulfate sulfurtransferase (VIMSS) compare
b4169 amiB +0.3 2.5 N-acetylmuramoyl-l-alanine amidase II (NCBI) compare
b0294 ykgK +0.3 1.3 predicted regulator (NCBI) compare
b1562 hokD +0.3 1.1 Qin prophage; small toxic polypeptide (NCBI) compare
b1656 sodB +0.3 1.4 superoxide dismutase, Fe (NCBI) compare
b2436 hemF +0.3 1.8 coproporphyrinogen III oxidase (NCBI) compare
b4353 yjiX +0.3 1.9 hypothetical protein (NCBI) compare
b4109 yjdA +0.3 2.4 conserved protein with nucleoside triphosphate hydrolase domain (NCBI) compare
b1185 dsbB +0.3 1.4 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b4135 yjdC +0.3 2.1 orf, hypothetical protein (VIMSS) compare
b2951 yggS +0.3 1.6 predicted enzyme (NCBI) compare
b1763 topB +0.3 0.9 DNA topoisomerase III (NCBI) compare
b1892 flhD +0.3 1.4 DNA-binding transcriptional dual regulator with FlhC (RefSeq) compare
b3977 tyrU +0.3 0.4 tRNA-Tyr (NCBI) compare
b2101 yegW +0.3 1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b0775 bioB +0.3 1.6 biotin synthase (NCBI) compare
b4293 fecI +0.3 1.7 KpLE2 phage-like element; RNA polymerase, sigma 19 factor (NCBI) compare
b2730 hypE +0.3 2.1 carbamoyl phosphate phosphatase, hydrogenase 3 maturation protein (RefSeq) compare
b1111 ycfQ +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b1335 ogt +0.3 1.3 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) compare


Specific Phenotypes

For 2 genes in this experiment

For carbon source Cytidine in Escherichia coli BW25113

For carbon source Cytidine across organisms