Experiment set65S22 for Escherichia coli BW25113
Ste41
Group: phageMedia: LB_plus_SM_buffer + MOI 10
Culturing: Keio_ML9a, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 4/1/24
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 16 genes in this experiment
For phage MOI 10 in Escherichia coli BW25113
For phage MOI 10 across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| CRISPRs | 1 |
| Formate hydrogenase | 1 |
| Glycine Biosynthesis | 1 |
| Glycine and Serine Utilization | 1 |
| Iron acquisition in Vibrio | 1 |
| Nitrate and nitrite ammonification | 1 |
| Siderophore Aerobactin | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-threonine degradation II | 2 | 2 | 1 |
| D-serine degradation | 3 | 3 | 1 |
| L-tryptophan degradation II (via pyruvate) | 3 | 3 | 1 |
| L-serine degradation | 3 | 3 | 1 |
| L-cysteine degradation II | 3 | 2 | 1 |
| L-methionine degradation II | 3 | 2 | 1 |
| felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis | 5 | 2 | 1 |
| L-threonine degradation I | 6 | 6 | 1 |
| L-methionine biosynthesis II | 6 | 5 | 1 |
| L-isoleucine biosynthesis I (from threonine) | 7 | 7 | 1 |
| glycine betaine degradation III | 7 | 4 | 1 |
| glycine betaine degradation I | 8 | 4 | 1 |
| L-mimosine degradation | 8 | 4 | 1 |
| glutathione-mediated detoxification I | 8 | 3 | 1 |
| superpathway of L-threonine metabolism | 18 | 16 | 2 |
| superpathway of L-isoleucine biosynthesis I | 13 | 13 | 1 |
| hypoglycin biosynthesis | 14 | 4 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis II | 15 | 13 | 1 |
| superpathway of branched chain amino acid biosynthesis | 17 | 17 | 1 |
| purine nucleobases degradation II (anaerobic) | 24 | 17 | 1 |