Experiment set65S22 for Escherichia coli BW25113

Compare to:

Ste41

Group: phage
Media: LB_plus_SM_buffer + MOI 10
Culturing: Keio_ML9a, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 4/1/24
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 18 genes in this experiment

For phage MOI 10 in Escherichia coli BW25113

For phage MOI 10 across organisms

SEED Subsystems

Subsystem #Specific
CRISPRs 1
Formate hydrogenase 1
Glycine Biosynthesis 1
Glycine and Serine Utilization 1
Iron acquisition in Vibrio 1
LOS core oligosaccharide biosynthesis 1
Nitrate and nitrite ammonification 1
Siderophore Aerobactin 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-threonine degradation II 2 2 1
D-serine degradation 3 3 1
L-serine degradation 3 3 1
L-tryptophan degradation II (via pyruvate) 3 3 1
L-methionine degradation II 3 2 1
L-cysteine degradation II 3 2 1
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis 5 2 1
L-threonine degradation I 6 6 1
L-methionine biosynthesis II 6 5 1
L-isoleucine biosynthesis I (from threonine) 7 7 1
glycine betaine degradation III 7 4 1
L-mimosine degradation 8 4 1
glycine betaine degradation I 8 4 1
glutathione-mediated detoxification I 8 3 1
superpathway of L-threonine metabolism 18 16 2
lipid A-core biosynthesis (E. coli K-12) 10 9 1
lipid A-core biosynthesis (Salmonella) 10 8 1
superpathway of L-isoleucine biosynthesis I 13 13 1
hypoglycin biosynthesis 14 4 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 1
superpathway of branched chain amino acid biosynthesis 17 17 1
purine nucleobases degradation II (anaerobic) 24 17 1
superpathway of Kdo2-lipid A biosynthesis 25 24 1