Experiment set65S21 for Escherichia coli BW25113

Compare to:

Ste41

200 most detrimental genes:

  gene name fitness t score description  
b1334 fnr +7.0 14.2 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b1256 ompW +7.0 48.7 outer membrane protein W (NCBI) compare
b3632 rfaQ +6.0 53.5 lipopolysaccharide core biosynthesis protein (NCBI) compare
b0222 lpcA +6.0 15.9 phosphoheptose isomerase (NCBI) compare
b3625 rfaY +6.0 27.7 lipopolysaccharide core biosynthesis protein (NCBI) compare
b1236 galU +5.8 27.3 glucose-1-phosphate uridylyltransferase (NCBI) compare
b3842 rfaH +5.6 24.8 transcriptional activator RfaH (NCBI) compare
b0688 pgm +5.4 16.6 phosphoglucomutase (NCBI) compare
b3620 rfaF +5.3 25.2 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3619 rfaD +5.3 19.8 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3052 rfaE +5.1 28.0 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3630 rfaP +5.0 39.1 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b3631 rfaG +5.0 42.6 glucosyltransferase I (NCBI) compare
b3621 rfaC +4.9 26.8 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3627 rfaI +4.8 33.8 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b1222 narX +4.8 8.0 sensory histidine kinase in two-component regulatory system with NarL (NCBI) compare
b0200 gmhB +4.8 6.4 hypothetical protein (NCBI) compare
b3628 rfaB +4.0 27.7 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b2512 yfgL +3.9 15.6 protein assembly complex, lipoprotein component (NCBI) compare
b4177 purA +3.8 6.5 adenylosuccinate synthetase (NCBI) compare
b4239 treC +3.6 9.4 trehalose-6-P hydrolase (NCBI) compare
b4313 fimE +3.3 19.0 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b3867 hemN +3.1 21.6 O2-independent coproporphyrinogen III oxidase (VIMSS) compare
b0683 fur +3.1 2.9 ferric uptake regulator (NCBI) compare
b3398 yrfF +3.0 10.4 predicted inner membrane protein (NCBI) compare
b4178 yjeB +2.7 13.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b0437 clpP +2.7 9.3 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b1084 rne +2.6 7.8 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b2808 gcvA +2.6 8.3 DNA-binding transcriptional dual regulator (NCBI) compare
b3725 pstB +2.5 2.3 phosphate transporter subunit (NCBI) compare
b2215 ompC +2.5 10.3 outer membrane porin protein C (NCBI) compare
b2846 yqeH +2.4 1.5 orf, hypothetical protein (VIMSS) compare
b4695 ykgT +2.4 3.0 no description compare
b3860 dsbA +2.4 16.3 periplasmic protein disulfide isomerase I (NCBI) compare
b2343 b2343 +2.4 1.5 orf, hypothetical protein (VIMSS) compare
b1235 rssB +2.3 12.5 response regulator of RpoS (NCBI) compare
b1855 lpxM +2.3 7.9 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b2830 nudH +2.3 3.2 dinucleoside polyphosphate hydrolase (NCBI) compare
b2814 metZ +2.3 2.2 tRNA-Met (NCBI) compare
b1476 fdnI +2.2 1.2 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b2572 rseA +2.2 5.4 anti-sigma factor (NCBI) compare
b3164 pnp +2.2 4.5 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b1836 yebV +2.2 4.7 orf, hypothetical protein (VIMSS) compare
b2684 mprA +2.2 8.3 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b3806 cyaA +2.2 7.2 adenylate cyclase (NCBI) compare
b0142 folK +2.2 0.7 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b1524 yneH +2.1 1.0 predicted glutaminase (NCBI) compare
b1151 ymfO +2.1 3.0 e14 prophage; conserved protein (RefSeq) compare
b0438 clpX +2.1 11.2 ATP-dependent protease ATP-binding subunit (NCBI) compare
b1279 yciS +2.1 1.6 conserved inner membrane protein (NCBI) compare
b0303 ykgI +2.1 1.8 orf, hypothetical protein (VIMSS) compare
b1159 mcrA +2.0 2.4 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b2285 nuoE +1.9 2.6 NADH dehydrogenase subunit E (NCBI) compare
b0740 tolB +1.9 7.0 translocation protein TolB precursor (NCBI) compare
b1540 ydfH +1.9 1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b3778 rep +1.9 12.8 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b4410 ecnA +1.9 1.7 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b2263 yfbB +1.8 1.8 predicted peptidase (NCBI) compare
b3387 dam +1.8 12.3 DNA adenine methylase (NCBI) compare
b1183 umuD +1.8 1.9 DNA polymerase V, subunit D (NCBI) compare
b1643 ydhI +1.8 2.8 predicted inner membrane protein (NCBI) compare
b2498 upp +1.8 5.0 uracil phosphoribosyltransferase (VIMSS) compare
b2286 nuoC +1.8 5.2 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b2261 menC +1.7 4.4 O-succinylbenzoate synthase (NCBI) compare
b2262 menB +1.7 5.6 naphthoate synthase (NCBI) compare
b2421 cysM +1.7 3.6 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b0178 hlpA +1.7 3.0 periplasmic chaperone (NCBI) compare
b2276 nuoN +1.7 3.5 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b4593 ymgI +1.7 1.3 hypothetical protein (RefSeq) compare
b1818 manY +1.7 2.4 mannose-specific enzyme IIC component of PTS (NCBI) compare
b3290 trkA +1.7 3.7 potassium transporter peripheral membrane component (NCBI) compare
b0101 yacG +1.6 2.3 zinc-binding protein (NCBI) compare
b1644 b1644 +1.6 2.9 putative membrane protein (VIMSS) compare
b4025 pgi +1.6 8.1 glucose-6-phosphate isomerase (NCBI) compare
b1001 yccE +1.6 0.6 hypothetical protein (NCBI) compare
b4529 ydbJ +1.6 0.9 hypothetical protein (NCBI) compare
b4345 mcrC +1.6 1.9 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b1257 yciE +1.6 0.5 hypothetical protein (NCBI) compare
b2934 cmtB +1.6 1.1 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b0487 cueR +1.6 3.8 DNA-binding transcriptional activator of copper-responsive regulon genes (NCBI) compare
b1347 ydaC +1.6 0.5 Rac prophage; predicted protein (NCBI) compare
b2055 wcaE +1.6 0.6 predicted glycosyl transferase (NCBI) compare
b1185 dsbB +1.5 3.9 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b1182 hlyE +1.5 0.7 hemolysin E (VIMSS) compare
b3555 yiaG +1.5 3.6 predicted transcriptional regulator (NCBI) compare
b1167 ymgC +1.5 0.4 hypothetical protein (NCBI) compare
b2682 ygaZ +1.5 4.2 predicted transporter (NCBI) compare
b1165 ymgA +1.5 1.0 hypothetical protein (NCBI) compare
b1497 b1497 +1.5 0.7 putative enzyme (VIMSS) compare
b1499 ydeO +1.5 0.7 predicted DNA-binding transcriptional acfivator (NCBI) compare
b4546 ypeB +1.5 0.8 hypothetical protein (NCBI) compare
b1831 proQ +1.5 3.7 putative solute/DNA competence effector (NCBI) compare
b0460 hha +1.5 3.7 modulator of gene expression, with H-NS (NCBI) compare
b1551 b1551 +1.5 0.5 orf, hypothetical protein (VIMSS) compare
b0296 ykgM +1.5 1.4 50S ribosomal protein L31 (NCBI) compare
b1576 ydfD +1.5 0.6 Qin prophage; predicted protein (NCBI) compare
b4444 rygA +1.5 3.5 unknown RNA (NCBI) compare
b4535 yniD +1.5 1.2 predicted protein (RefSeq) compare
b1531 marA +1.5 2.3 multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS) compare
b1942 fliJ +1.5 1.1 flagellar biosynthesis chaperone (NCBI) compare
b2409 yfeR +1.5 3.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b1865 nudB +1.5 1.8 dATP pyrophosphohydrolase (NCBI) compare
b4561 insM +1.5 3.4 transposase (RefSeq) compare
b2905 gcvT +1.5 4.5 glycine cleavage system aminomethyltransferase T (NCBI) compare
b1902 yecI +1.5 3.5 predicted ferritin-like protein (NCBI) compare
b3844 fre +1.5 5.5 NAD(P)H-flavin reductase (NCBI) compare
b0739 tolA +1.5 3.6 cell envelope integrity inner membrane protein TolA (NCBI) compare
b3658 selC +1.4 1.3 tRNA-Sec (NCBI) compare
b1363 trkG +1.4 0.9 Rac prophage; potassium transporter subunit (NCBI) compare
b0310 ykgH +1.4 2.3 predicted inner membrane protein (NCBI) compare
b2824 ygdB +1.4 1.7 orf, hypothetical protein (VIMSS) compare
b3405 ompR +1.4 3.5 osmolarity response regulator (NCBI) compare
b0903 pflB +1.4 8.1 pyruvate formate lyase I (NCBI) compare
b1111 ycfQ +1.4 1.5 orf, hypothetical protein (VIMSS) compare
b1351 racC +1.4 0.7 Rac prophage; predicted protein (NCBI) compare
b1664 ydhQ +1.4 4.3 hypothetical protein (NCBI) compare
b2567 rnc +1.4 0.9 ribonuclease III (NCBI) compare
b1950 fliR +1.4 3.3 flagellar biosynthesis protein R (NCBI) compare
b4675 yoaJ +1.4 2.9 expressed protein (RefSeq) compare
b2460 eutQ +1.4 3.2 hypothetical protein (NCBI) compare
b4451 ryhB +1.4 1.4 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b0234 yafP +1.4 2.6 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b3410 yhgG +1.4 4.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b1427 rimL +1.4 2.4 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b1409 ynbB +1.4 0.9 predicted CDP-diglyceride synthase (NCBI) compare
b0143 pcnB +1.4 4.7 poly(A) polymerase I (VIMSS) compare
b2579 yfiD +1.3 1.6 pyruvate formate lyase subunit (NCBI) compare
b0118 acnB +1.3 1.2 aconitate hydratase (NCBI) compare
b3121 yhaC +1.3 0.6 hypothetical protein (NCBI) compare
b2990 hybG +1.3 1.1 hydrogenase 2 accessory protein (NCBI) compare
b3764 yifE +1.3 2.6 hypothetical protein (NCBI) compare
b2617 smpA +1.3 2.7 small membrane protein A (VIMSS) compare
b1690 ydiM +1.3 2.4 predicted transporter (NCBI) compare
b0902 pflA +1.3 3.9 pyruvate formate lyase activating enzyme 1 (NCBI) compare
b1795 yeaQ +1.3 3.4 conserved inner membrane protein (NCBI) compare
b4443 gcvB +1.3 0.4 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b4246 pyrL +1.3 1.1 pyrBI operon leader peptide (NCBI) compare
b2012 yeeD +1.3 2.2 hypothetical protein (NCBI) compare
b0049 apaH +1.3 3.2 diadenosinetetraphosphatase (NCBI) compare
b4494 arpB +1.3 2.5 no description compare
b3609 secB +1.3 2.4 export protein SecB (NCBI) compare
b1085 b1085 +1.3 1.2 orf, hypothetical protein (VIMSS) compare
b2297 pta +1.3 0.7 phosphate acetyltransferase (NCBI) compare
b2847 yqeI +1.3 0.8 predicted transcriptional regulator (NCBI) compare
b1604 ydgH +1.3 2.2 hypothetical protein (NCBI) compare
b2528 iscA +1.3 1.1 iron-sulfur cluster assembly protein (NCBI) compare
b1785 yeaI +1.3 2.9 predicted diguanylate cyclase (NCBI) compare
b0249 ykfF +1.3 1.3 CP4-6 prophage; predicted protein (NCBI) compare
b3022 b3022 +1.3 4.4 predicted cyanide hydratase (NCBI) compare
b1129 phoQ +1.3 1.1 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b0450 glnK +1.3 2.2 nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB (NCBI) compare
b2284 nuoF +1.3 4.2 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b0603 ybdO +1.3 0.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b0738 tolR +1.3 3.4 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1696 ydiP +1.3 2.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b2278 nuoL +1.3 5.9 NADH dehydrogenase subunit L (NCBI) compare
b0125 hpt +1.2 3.0 hypoxanthine phosphoribosyltransferase (VIMSS) compare
b1466 narW +1.2 3.5 nitrate reductase 2 (NRZ), delta subunit (assembly subunit) (NCBI) compare
b1677 lpp +1.2 1.2 murein lipoprotein (NCBI) compare
b2623 yfjH +1.2 4.5 CP4-57 prophage; predicted protein (NCBI) compare
b1443 ydcV +1.2 2.1 predicted spermidine/putrescine transporter subunit (NCBI) compare
b1833 yebS +1.2 3.3 conserved inner membrane protein (NCBI) compare
b0737 tolQ +1.2 2.7 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0279 yagM +1.2 0.7 CP4-6 prophage; predicted protein (NCBI) compare
b0379 yaiY +1.2 2.6 predicted inner membrane protein (NCBI) compare
b2952 yggT +1.2 4.1 predicted inner membrane protein (NCBI) compare
b3442 yhhZ +1.2 0.5 hypothetical protein (NCBI) compare
b2264 menD +1.2 3.7 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b0774 bioA +1.2 2.8 adenosylmethionine--8-amino-7-oxononanoate transaminase (NCBI) compare
b0439 lon +1.2 4.5 DNA-binding ATP-dependent protease La (NCBI) compare
b1143 ymfI +1.2 0.9 orf, hypothetical protein (VIMSS) compare
b2583 yfiP +1.2 2.6 orf, hypothetical protein (VIMSS) compare
b1854 pykA +1.2 4.2 pyruvate kinase (NCBI) compare
b2004 yeeU +1.2 1.4 CP4-44 prophage; antitoxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b4660 yhiL +1.2 0.6 no description compare
b1130 phoP +1.2 1.0 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b3087 ygjR +1.2 3.5 predicted NAD(P)-binding dehydrogenase (RefSeq) compare
b1496 yddA +1.2 5.5 fused predicted multidrug transporter subunits of ABC superfamily: membrane component/ATP-binding component (NCBI) compare
b1234 rssA +1.2 3.4 conserved protein (RefSeq) compare
b2510 b2510 +1.2 2.0 orf, hypothetical protein (VIMSS) compare
b2531 iscR +1.2 0.9 DNA-binding transcriptional repressor (NCBI) compare
b3504 yhiS +1.2 0.5 hypothetical protein (RefSeq) compare
b4128 yjdK +1.2 0.7 hypothetical protein (NCBI) compare
b2724 hycB +1.1 2.6 hydrogenase 3, Fe-S subunit (NCBI) compare
b3930 menA +1.1 7.2 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b3386 rpe +1.1 1.0 ribulose-phosphate 3-epimerase (NCBI) compare
b4172 hfq +1.1 0.9 RNA-binding protein Hfq (NCBI) compare
b4155 yjeA +1.1 0.6 putative lysyl-tRNA synthetase (VIMSS) compare
b0726 sucA +1.1 2.5 alpha-ketoglutarate decarboxylase (NCBI) compare
b1040 csgD +1.1 1.4 DNA-binding transcriptional activator in two-component regulatory system (NCBI) compare
b2352 yfdI +1.1 0.7 CPS-53 (KpLE1) prophage; predicted inner membrane protein (NCBI) compare
b1048 mdoG +1.1 4.2 glucan biosynthesis protein, periplasmic (NCBI) compare
b1264 trpE +1.1 2.7 anthranilate synthase component I (NCBI) compare
b0896 dmsC +1.1 4.1 dimethyl sulfoxide reductase, anaerobic, subunit C (NCBI) compare
b1811 yoaH +1.1 1.3 hypothetical protein (NCBI) compare
b1970 yedX +1.1 3.7 hypothetical protein (NCBI) compare
b1575 dicB +1.1 1.1 Qin prophage; cell division inhibition protein (NCBI) compare
b1428 ydcK +1.1 3.1 predicted enzyme (NCBI) compare
b1984 asnW +1.1 0.7 tRNA-Asn (NCBI) compare
b0500 ybbD +1.1 0.4 hypothetical protein (RefSeq) compare


Specific Phenotypes

For 13 genes in this experiment

For phage MOI 10 in Escherichia coli BW25113

For phage MOI 10 across organisms