Experiment set61S71 for Escherichia coli BW25113
Laval1374
Group: phageMedia: LB_plus_SM_buffer + MOI 10
Culturing: Keio_ML9a, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 8/7/23
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 17 genes in this experiment
For phage MOI 10 in Escherichia coli BW25113
For phage MOI 10 across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Colanic acid biosynthesis | 7 |
| Alkylphosphonate utilization | 1 |
| Iron acquisition in Vibrio | 1 |
| Peptidyl-prolyl cis-trans isomerase | 1 |
| Potassium homeostasis | 1 |
| Siderophore Aerobactin | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Lipopolysaccharide biosynthesis
- Pentose and glucuronate interconversions
- Ascorbate and aldarate metabolism
- Puromycin biosynthesis
- Starch and sucrose metabolism
- Nucleotide sugars metabolism
- T cell receptor signaling pathway
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| UDP-α-D-glucuronate biosynthesis (from UDP-glucose) | 1 | 1 | 1 |
| UDP-α-D-xylose biosynthesis | 2 | 1 | 1 |
| colanic acid (Escherichia coli K12) biosynthesis | 11 | 10 | 4 |
| superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis | 6 | 5 | 1 |
| UDP-sugars interconversion | 9 | 2 | 1 |
| teichuronic acid biosynthesis (B. subtilis 168) | 9 | 2 | 1 |
| colanic acid building blocks biosynthesis | 11 | 11 | 1 |