Experiment set60S330 for Escherichia coli BW25113

Compare to:

TN_15CS_warm37C_5dbl_rep3

200 most important genes:

  gene name fitness t score description  
b0920 ycbC -8.7 -6.0 conserved inner membrane protein (NCBI) compare
b3871 bipA -6.5 -22.4 GTP-binding protein (NCBI) compare
b3390 aroK -5.7 -3.9 shikimate kinase I (NCBI) compare
b3341 rpsG -5.4 -3.3 30S ribosomal protein S7 (NCBI) compare
b3183 obgE -4.8 -3.2 GTPase involved in cell partioning and DNA repair (NCBI) compare
b1175 minD -4.4 -6.9 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b3493 pitA -4.2 -13.1 phosphate transporter, low-affinity (NCBI) compare
b3162 deaD -4.1 -7.3 inducible ATP-independent RNA helicase (VIMSS) compare
b0149 mrcB -4.1 -19.9 penicillin-binding protein 1b (NCBI) compare
b0222 lpcA -3.9 -2.7 phosphoheptose isomerase (NCBI) compare
b3652 recG -3.9 -16.9 ATP-dependent DNA helicase (NCBI) compare
b1863 ruvC -3.8 -2.6 Holliday junction resolvase (NCBI) compare
b0049 apaH -3.6 -7.2 diadenosinetetraphosphatase (NCBI) compare
b4234 yjgA -3.5 -9.9 hypothetical protein (NCBI) compare
b3619 rfaD -3.3 -4.5 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3204 ptsN -3.2 -6.8 sugar-specific enzyme IIA component of PTS (NCBI) compare
b4202 rpsR -3.1 -3.6 30S ribosomal protein S18 (NCBI) compare
b3052 rfaE -3.1 -7.9 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b0888 trxB -3.0 -4.9 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b0912 ihfB -3.0 -7.2 integration host factor subunit beta (NCBI) compare
b3621 rfaC -3.0 -6.0 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3620 rfaF -3.0 -10.3 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b2912 ygfA -2.9 -6.0 putative ligase (VIMSS) compare
b2830 nudH -2.9 -3.3 dinucleoside polyphosphate hydrolase (NCBI) compare
b0051 ksgA -2.8 -7.9 dimethyladenosine transferase (NCBI) compare
b0082 mraW -2.7 -6.5 S-adenosyl-methyltransferase (NCBI) compare
b1176 minC -2.7 -4.9 septum formation inhibitor (NCBI) compare
b1291 sapD -2.7 -2.5 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2935 tktA -2.6 -1.8 transketolase 1, thiamin-binding (NCBI) compare
b3781 trxA -2.6 -9.0 thioredoxin 1 (VIMSS) compare
b0439 lon -2.6 -8.3 DNA-binding ATP-dependent protease La (NCBI) compare
b2576 srmB -2.5 -4.5 ATP-dependent RNA helicase (NCBI) compare
b4146 yjeK -2.5 -2.4 predicted lysine aminomutase (NCBI) compare
b3261 fis -2.4 -1.7 DNA-binding protein Fis (NCBI) compare
b3500 gor -2.4 -3.5 glutathione reductase (NCBI) compare
b1187 fadR -2.4 -2.3 fatty acid metabolism regulator (NCBI) compare
b0014 dnaK -2.4 -5.4 molecular chaperone DnaK (NCBI) compare
b3636 rpmG -2.4 -3.1 50S ribosomal protein L33 (NCBI) compare
b3414 gntY -2.4 -7.9 predicted gluconate transport associated protein (NCBI) compare
b2937 speB -2.4 -10.1 agmatinase (NCBI) compare
b2518 ndk -2.3 -4.2 nucleoside diphosphate kinase (NCBI) compare
b2479 gcvR -2.3 -6.9 transcriptional regulation of gcv operon (VIMSS) compare
b2501 ppk -2.3 -11.4 polyphosphate kinase (NCBI) compare
b3229 sspA -2.3 -5.0 stringent starvation protein A (NCBI) compare
b3472 b3472 -2.3 -14.2 orf, hypothetical protein (VIMSS) compare
b3609 secB -2.2 -2.0 export protein SecB (NCBI) compare
b1865 nudB -2.2 -2.6 dATP pyrophosphohydrolase (NCBI) compare
b3613 yibP -2.2 -2.2 putative membrane protein (VIMSS) compare
b2620 smpB -2.2 -2.0 SsrA-binding protein (NCBI) compare
b3863 polA -2.2 -1.5 DNA polymerase I (NCBI) compare
b2318 truA -2.2 -3.7 tRNA pseudouridine synthase A (NCBI) compare
b3916 pfkA -2.1 -5.6 6-phosphofructokinase (NCBI) compare
b1293 sapB -2.1 -2.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1286 rnb -2.1 -4.0 exoribonuclease II (NCBI) compare
b2569 lepA -2.1 -6.0 GTP-binding protein LepA (NCBI) compare
b3839 tatC -2.1 -9.0 TatABCE protein translocation system subunit (NCBI) compare
b3164 pnp -2.0 -3.1 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b0759 galE -2.0 -5.7 UDP-galactose-4-epimerase (VIMSS) compare
b1861 ruvA -2.0 -1.3 Holliday junction DNA helicase motor protein (NCBI) compare
b1236 galU -2.0 -3.0 glucose-1-phosphate uridylyltransferase (NCBI) compare
b1882 cheY -2.0 -1.0 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1658 purR -2.0 -8.5 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b3849 trkH -2.0 -14.2 potassium transporter (NCBI) compare
b1506 yneL -1.9 -2.2 predicted transcriptional regulator (NCBI) compare
b2350 yfdG -1.9 -4.9 CPS-53 (KpLE1) prophage; bactoprenol-linked glucose translocase (flippase) (NCBI) compare
b2330 yfcB -1.9 -1.3 putative adenine-specific methylase (VIMSS) compare
b2814 metZ -1.9 -2.7 tRNA-Met (NCBI) compare
b0767 ybhE -1.8 -3.8 6-phosphogluconolactonase (NCBI) compare
b0200 gmhB -1.8 -2.1 hypothetical protein (NCBI) compare
b3868 glnG -1.8 -7.2 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b3026 qseC -1.8 -6.8 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b0623 cspE -1.8 -2.2 cold shock protein E (NCBI) compare
b3290 trkA -1.8 -2.3 potassium transporter peripheral membrane component (NCBI) compare
b3741 gidA -1.7 -5.5 glucose-inhibited division protein A (NCBI) compare
b0053 surA -1.7 -7.3 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b2007 yeeX -1.7 -2.7 conserved protein (RefSeq) compare
b1095 fabF -1.7 -9.5 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b0737 tolQ -1.7 -4.1 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3205 yhbJ -1.6 -5.6 hypothetical protein (NCBI) compare
b4375 prfC -1.6 -2.2 peptide chain release factor 3 (NCBI) compare
b3706 trmE -1.6 -6.3 tRNA modification GTPase (NCBI) compare
b0088 murD -1.6 -1.3 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b3169 nusA -1.6 -3.6 transcription elongation factor NusA (NCBI) compare
b1290 sapF -1.5 -1.5 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3728 pstS -1.5 -5.9 phosphate transporter subunit (NCBI) compare
b2688 gshA -1.5 -9.0 glutamate--cysteine ligase (NCBI) compare
b1850 eda -1.5 -1.8 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b0738 tolR -1.5 -3.7 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0630 lipB -1.5 -1.0 protein of lipoate biosynthesis (VIMSS) compare
b3961 oxyR -1.5 -10.4 DNA-binding transcriptional dual regulator (NCBI) compare
b1936 intG -1.5 -1.8 predicted defective phage integrase (pseudogene) (RefSeq) compare
b3792 wzxE -1.5 -8.3 O-antigen translocase (NCBI) compare
b2567 rnc -1.4 -0.9 ribonuclease III (NCBI) compare
b3198 kdsC -1.4 -5.9 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b2168 fruK -1.4 -0.8 1-phosphofructokinase (NCBI) compare
b1292 sapC -1.4 -2.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0226 dinJ -1.4 -1.6 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b3035 tolC -1.4 -7.9 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b4171 miaA -1.3 -4.0 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b2498 upp -1.3 -3.1 uracil phosphoribosyltransferase (VIMSS) compare
b0677 nagA -1.3 -5.7 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b0406 tgt -1.3 -6.3 queuine tRNA-ribosyltransferase (NCBI) compare
b3386 rpe -1.3 -0.9 ribulose-phosphate 3-epimerase (NCBI) compare
b0167 glnD -1.2 -5.6 PII uridylyl-transferase (NCBI) compare
b0114 aceE -1.2 -1.1 pyruvate dehydrogenase subunit E1 (NCBI) compare
b0740 tolB -1.2 -3.9 translocation protein TolB precursor (NCBI) compare
b2846 yqeH -1.2 -0.7 orf, hypothetical protein (VIMSS) compare
b0890 ftsK -1.2 -2.7 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b0232 yafN -1.2 -0.5 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b4208 cycA -1.2 -8.0 D-alanine/D-serine/glycine transporter (NCBI) compare
b1105 ycfM -1.2 -3.7 predicted outer membrane lipoprotein (NCBI) compare
b3237 argR -1.2 -4.5 arginine repressor (NCBI) compare
b2071 yegJ -1.1 -1.5 hypothetical protein (NCBI) compare
b1712 ihfA -1.1 -1.5 integration host factor subunit alpha (NCBI) compare
b0949 uup -1.1 -6.5 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b4179 vacB -1.1 -5.7 putative enzyme (VIMSS) compare
b1269 rluB -1.1 -3.1 23S rRNA pseudouridylate synthase (NCBI) compare
b1613 manA -1.1 -2.4 mannose-6-phosphate isomerase (VIMSS) compare
b0741 pal -1.1 -3.2 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b0605 ahpC -1.1 -2.2 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b1855 lpxM -1.1 -3.8 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b0469 apt -1.1 -6.0 adenine phosphoribosyltransferase (NCBI) compare
b3842 rfaH -1.0 -4.9 transcriptional activator RfaH (NCBI) compare
b4402 yjjY -1.0 -1.0 hypothetical protein (NCBI) compare
b3816 corA -1.0 -7.3 magnesium/nickel/cobalt transporter (NCBI) compare
b4235 pmbA -1.0 -8.1 predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 (NCBI) compare
b3244 tldD -1.0 -8.7 predicted peptidase (NCBI) compare
b4685 yrbN -1.0 -2.4 expressed protein (RefSeq) compare
b0758 galT -1.0 -3.0 galactose-1-phosphate uridylyltransferase (NCBI) compare
b0585 fes -1.0 -4.9 enterobactin/ferric enterobactin esterase (NCBI) compare
b1323 tyrR -1.0 -4.7 DNA-binding transcriptional dual regulator, tyrosine-binding (NCBI) compare
b3616 tdh -1.0 -5.8 L-threonine 3-dehydrogenase (NCBI) compare
b3726 pstA -1.0 -4.8 phosphate transporter subunit (NCBI) compare
b1025 ycdT -1.0 -1.6 predicted diguanylate cyclase (NCBI) compare
b1213 ychQ -1.0 -2.0 predicted transcriptional regulator (NCBI) compare
b1676 pykF -1.0 -2.6 pyruvate kinase (NCBI) compare
b3651 trmH -1.0 -3.3 tRNA (Guanosine-2'-O-)-methyltransferase (NCBI) compare
b2894 xerD -1.0 -0.6 site-specific tyrosine recombinase XerD (NCBI) compare
b0739 tolA -1.0 -3.1 cell envelope integrity inner membrane protein TolA (NCBI) compare
b3247 cafA -0.9 -7.7 bundles of cytoplasmic filaments (VIMSS) compare
b1542 ydfI -0.9 -2.9 predicted mannonate dehydrogenase (NCBI) compare
b1109 ndh -0.9 -3.9 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b3650 spoT -0.9 -5.1 bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI) compare
b2897 ygfY -0.9 -3.4 hypothetical protein (NCBI) compare
b4201 priB -0.9 -3.5 primosomal replication protein N (NCBI) compare
b3933 ftsN -0.9 -5.5 essential cell division protein (NCBI) compare
b2947 gshB -0.9 -3.5 glutathione synthetase (NCBI) compare
b3149 yraO -0.9 -6.8 DnaA initiator-associating factor for replication initiation (NCBI) compare
b2910 zapA -0.9 -5.5 protein that localizes to the cytokinetic ring (NCBI) compare
b4520 ymgF -0.9 -1.9 hypothetical protein (NCBI) compare
b3838 tatB -0.9 -4.3 sec-independent translocase (NCBI) compare
b3700 recF -0.9 -6.8 recombination protein F (NCBI) compare
b3348 slyX -0.9 -3.4 hypothetical protein (NCBI) compare
b2392 mntH -0.9 -5.4 manganese transport protein MntH (NCBI) compare
b3389 aroB -0.8 -6.3 3-dehydroquinate synthase (NCBI) compare
b3163 nlpI -0.8 -3.6 hypothetical protein (NCBI) compare
b1830 prc -0.8 -4.3 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b2319 usg -0.8 -3.9 hypothetical protein (NCBI) compare
b2175 spr -0.8 -3.0 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b3791 rffA -0.8 -5.9 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b0237 pepD -0.8 -5.7 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b2892 recJ -0.8 -5.6 ssDNA exonuclease, 5' --> 3'-specific (NCBI) compare
b0386 proC -0.8 -4.5 pyrroline-5-carboxylate reductase (NCBI) compare
b1942 fliJ -0.8 -1.1 flagellar biosynthesis chaperone (NCBI) compare
b2017 yefM -0.8 -1.3 orf, hypothetical protein (VIMSS) compare
b4180 rlmB -0.8 -4.9 23S rRNA (Gm2251)-methyltransferase (NCBI) compare
b4655 ythA -0.8 -2.0 expressed protein (RefSeq) compare
b3465 yhhF -0.8 -4.2 predicted methyltransferase (NCBI) compare
b1493 gadB -0.8 -0.8 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b4557 yidD -0.8 -3.0 hypothetical protein (NCBI) compare
b1332 ynaJ -0.8 -0.5 predicted inner membrane protein (NCBI) compare
b2329 aroC -0.8 -3.8 chorismate synthase (NCBI) compare
b0143 pcnB -0.8 -3.7 poly(A) polymerase I (VIMSS) compare
b3387 dam -0.8 -6.5 DNA adenine methylase (NCBI) compare
b1480 sra -0.8 -1.1 30S ribosomal subunit protein S22 (NCBI) compare
b4177 purA -0.8 -1.1 adenylosuccinate synthetase (NCBI) compare
b2820 recB -0.8 -0.5 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b0683 fur -0.8 -1.3 ferric uptake regulator (NCBI) compare
b3630 rfaP -0.8 -5.8 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b4384 deoD -0.8 -5.0 purine nucleoside phosphorylase (NCBI) compare
b1860 ruvB -0.8 -0.5 Holliday junction DNA helicase B (NCBI) compare
b3617 kbl -0.8 -4.7 2-amino-3-ketobutyrate coenzyme A ligase (NCBI) compare
b1709 btuD -0.7 -1.0 vitamin B12-transporter ATPase (NCBI) compare
b3462 ftsX -0.7 -3.8 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0472 recR -0.7 -4.3 recombination protein RecR (NCBI) compare
b3794 rffM -0.7 -4.8 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b4414 tff -0.7 -1.0 unknown RNA (NCBI) compare
b2565 recO -0.7 -2.9 DNA repair protein RecO (NCBI) compare
b3935 priA -0.7 -1.8 primosome assembly protein PriA (NCBI) compare
b1920 fliY -0.7 -1.8 cystine transporter subunit (NCBI) compare
b1258 yciF -0.7 -0.6 hypothetical protein (NCBI) compare
b2699 recA -0.7 -0.5 recombinase A (NCBI) compare
b2582 trxC -0.7 -2.3 thioredoxin 2 (NCBI) compare
b0405 queA -0.7 -4.1 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI) compare
b3146 yraL -0.7 -4.2 predicted methyltransferase (NCBI) compare
b1161 ycgX -0.7 -0.8 hypothetical protein (NCBI) compare
b3388 damX -0.7 -5.1 hypothetical protein (NCBI) compare
b3649 rpoZ -0.7 -1.8 DNA-directed RNA polymerase subunit omega (NCBI) compare
b3790 rffC -0.7 -4.9 TDP-fucosamine acetyltransferase (RefSeq) compare
b3869 glnL -0.7 -4.5 sensory histidine kinase in two-component regulatory system with GlnG (NCBI) compare


Specific Phenotypes

For 3 genes in this experiment