Experiment set60S309 for Escherichia coli BW25113

Compare to:

TN_18CS_warm37C_5dbl_rep3

200 most important genes:

  gene name fitness t score description  
b0920 ycbC -8.9 -6.1 conserved inner membrane protein (NCBI) compare
b3871 bipA -6.8 -22.9 GTP-binding protein (NCBI) compare
b3636 rpmG -5.4 -4.1 50S ribosomal protein L33 (NCBI) compare
b3162 deaD -5.0 -5.9 inducible ATP-independent RNA helicase (VIMSS) compare
b2576 srmB -5.0 -3.4 ATP-dependent RNA helicase (NCBI) compare
b2620 smpB -4.5 -3.1 SsrA-binding protein (NCBI) compare
b3341 rpsG -4.3 -3.4 30S ribosomal protein S7 (NCBI) compare
b3390 aroK -4.0 -3.8 shikimate kinase I (NCBI) compare
b1175 minD -3.8 -7.7 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b3652 recG -3.7 -17.0 ATP-dependent DNA helicase (NCBI) compare
b0439 lon -3.4 -8.1 DNA-binding ATP-dependent protease La (NCBI) compare
b2479 gcvR -3.4 -10.0 transcriptional regulation of gcv operon (VIMSS) compare
b3164 pnp -3.4 -5.5 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b0683 fur -3.4 -2.0 ferric uptake regulator (NCBI) compare
b3979 thrT -3.1 -1.1 tRNA-Thr (NCBI) compare
b0049 apaH -3.1 -6.1 diadenosinetetraphosphatase (NCBI) compare
b3261 fis -3.0 -2.9 DNA-binding protein Fis (NCBI) compare
b3229 sspA -2.9 -6.6 stringent starvation protein A (NCBI) compare
b1865 nudB -2.9 -2.8 dATP pyrophosphohydrolase (NCBI) compare
b2350 yfdG -2.9 -4.6 CPS-53 (KpLE1) prophage; bactoprenol-linked glucose translocase (flippase) (NCBI) compare
b3204 ptsN -2.9 -9.1 sugar-specific enzyme IIA component of PTS (NCBI) compare
b0222 lpcA -2.9 -3.4 phosphoheptose isomerase (NCBI) compare
b3613 yibP -2.9 -2.8 putative membrane protein (VIMSS) compare
b0088 murD -2.9 -2.0 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b0149 mrcB -2.8 -18.9 penicillin-binding protein 1b (NCBI) compare
b3619 rfaD -2.8 -6.1 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b4234 yjgA -2.7 -10.1 hypothetical protein (NCBI) compare
b3792 wzxE -2.7 -12.8 O-antigen translocase (NCBI) compare
b3493 pitA -2.6 -12.7 phosphate transporter, low-affinity (NCBI) compare
b3863 polA -2.6 -1.8 DNA polymerase I (NCBI) compare
b3183 obgE -2.6 -2.9 GTPase involved in cell partioning and DNA repair (NCBI) compare
b1176 minC -2.6 -6.2 septum formation inhibitor (NCBI) compare
b4375 prfC -2.6 -1.8 peptide chain release factor 3 (NCBI) compare
b2937 speB -2.6 -8.8 agmatinase (NCBI) compare
b2330 yfcB -2.5 -1.7 putative adenine-specific methylase (VIMSS) compare
b0051 ksgA -2.4 -6.8 dimethyladenosine transferase (NCBI) compare
b2498 upp -2.3 -4.6 uracil phosphoribosyltransferase (VIMSS) compare
b1861 ruvA -2.3 -1.6 Holliday junction DNA helicase motor protein (NCBI) compare
b1236 galU -2.3 -2.7 glucose-1-phosphate uridylyltransferase (NCBI) compare
b3609 secB -2.2 -2.1 export protein SecB (NCBI) compare
b1290 sapF -2.2 -2.5 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2814 metZ -2.1 -2.8 tRNA-Met (NCBI) compare
b3868 glnG -2.1 -7.7 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b3290 trkA -2.1 -1.4 potassium transporter peripheral membrane component (NCBI) compare
b1292 sapC -2.0 -2.6 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3916 pfkA -2.0 -5.7 6-phosphofructokinase (NCBI) compare
b4685 yrbN -2.0 -4.5 expressed protein (RefSeq) compare
b1187 fadR -2.0 -3.8 fatty acid metabolism regulator (NCBI) compare
b4202 rpsR -2.0 -2.6 30S ribosomal protein S18 (NCBI) compare
b3621 rfaC -2.0 -7.7 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b2569 lepA -2.0 -5.2 GTP-binding protein LepA (NCBI) compare
b1252 tonB -2.0 -3.3 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b2501 ppk -2.0 -9.5 polyphosphate kinase (NCBI) compare
b1095 fabF -1.9 -9.8 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b3414 gntY -1.9 -6.5 predicted gluconate transport associated protein (NCBI) compare
b3781 trxA -1.9 -6.4 thioredoxin 1 (VIMSS) compare
b0888 trxB -1.9 -3.8 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b1860 ruvB -1.8 -1.2 Holliday junction DNA helicase B (NCBI) compare
b0912 ihfB -1.8 -2.8 integration host factor subunit beta (NCBI) compare
b2830 nudH -1.8 -2.8 dinucleoside polyphosphate hydrolase (NCBI) compare
b1291 sapD -1.8 -1.2 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3849 trkH -1.8 -13.0 potassium transporter (NCBI) compare
b3811 xerC -1.7 -1.2 site-specific tyrosine recombinase XerC (NCBI) compare
b0232 yafN -1.7 -0.8 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b0082 mraW -1.7 -5.0 S-adenosyl-methyltransferase (NCBI) compare
b3706 trmE -1.7 -6.2 tRNA modification GTPase (NCBI) compare
b4676 yoaK -1.7 -0.8 expressed protein (RefSeq) compare
b2518 ndk -1.7 -1.8 nucleoside diphosphate kinase (NCBI) compare
b0767 ybhE -1.6 -6.5 6-phosphogluconolactonase (NCBI) compare
b2318 truA -1.6 -4.1 tRNA pseudouridine synthase A (NCBI) compare
b2912 ygfA -1.6 -3.3 putative ligase (VIMSS) compare
b1286 rnb -1.6 -3.0 exoribonuclease II (NCBI) compare
b2846 yqeH -1.6 -1.0 orf, hypothetical protein (VIMSS) compare
b0737 tolQ -1.6 -3.3 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b2363 yfdT -1.6 -3.7 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b3052 rfaE -1.6 -4.8 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b0630 lipB -1.6 -1.1 protein of lipoate biosynthesis (VIMSS) compare
b3198 kdsC -1.6 -6.6 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b3728 pstS -1.6 -5.6 phosphate transporter subunit (NCBI) compare
b0890 ftsK -1.6 -1.8 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b1676 pykF -1.5 -4.4 pyruvate kinase (NCBI) compare
b3620 rfaF -1.5 -6.7 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b0200 gmhB -1.5 -1.0 hypothetical protein (NCBI) compare
b3961 oxyR -1.5 -11.5 DNA-binding transcriptional dual regulator (NCBI) compare
b1129 phoQ -1.5 -2.4 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b2528 iscA -1.5 -2.2 iron-sulfur cluster assembly protein (NCBI) compare
b4402 yjjY -1.5 -2.0 hypothetical protein (NCBI) compare
b3237 argR -1.4 -4.7 arginine repressor (NCBI) compare
b2935 tktA -1.4 -1.4 transketolase 1, thiamin-binding (NCBI) compare
b3169 nusA -1.4 -3.8 transcription elongation factor NusA (NCBI) compare
b2531 iscR -1.4 -1.9 DNA-binding transcriptional repressor (NCBI) compare
b0990 cspG -1.4 -1.3 DNA-binding transcriptional regulator (NCBI) compare
b3839 tatC -1.4 -6.8 TatABCE protein translocation system subunit (NCBI) compare
b0406 tgt -1.4 -4.9 queuine tRNA-ribosyltransferase (NCBI) compare
b0759 galE -1.4 -5.4 UDP-galactose-4-epimerase (VIMSS) compare
b1850 eda -1.4 -1.6 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b1293 sapB -1.4 -1.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0605 ahpC -1.3 -2.7 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b0014 dnaK -1.3 -3.5 molecular chaperone DnaK (NCBI) compare
b1269 rluB -1.3 -5.3 23S rRNA pseudouridylate synthase (NCBI) compare
b3741 gidA -1.3 -4.7 glucose-inhibited division protein A (NCBI) compare
b3616 tdh -1.3 -7.0 L-threonine 3-dehydrogenase (NCBI) compare
b3035 tolC -1.3 -7.7 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b4552 yrhC -1.3 -1.4 no description compare
b0226 dinJ -1.3 -1.6 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b2688 gshA -1.3 -6.4 glutamate--cysteine ligase (NCBI) compare
b0740 tolB -1.3 -3.7 translocation protein TolB precursor (NCBI) compare
b4208 cycA -1.3 -8.8 D-alanine/D-serine/glycine transporter (NCBI) compare
b3205 yhbJ -1.2 -5.4 hypothetical protein (NCBI) compare
b3500 gor -1.2 -3.3 glutathione reductase (NCBI) compare
b2297 pta -1.2 -0.8 phosphate acetyltransferase (NCBI) compare
b2329 aroC -1.2 -5.9 chorismate synthase (NCBI) compare
b4180 rlmB -1.2 -7.0 23S rRNA (Gm2251)-methyltransferase (NCBI) compare
b0739 tolA -1.2 -4.2 cell envelope integrity inner membrane protein TolA (NCBI) compare
b4146 yjeK -1.2 -2.6 predicted lysine aminomutase (NCBI) compare
b3026 qseC -1.1 -4.7 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b3778 rep -1.1 -6.6 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b3870 glnA -1.1 -2.6 glutamine synthetase (NCBI) compare
b3816 corA -1.1 -7.8 magnesium/nickel/cobalt transporter (NCBI) compare
b1863 ruvC -1.1 -1.5 Holliday junction resolvase (NCBI) compare
b0053 surA -1.1 -5.6 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b0237 pepD -1.1 -7.5 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b4179 vacB -1.1 -6.2 putative enzyme (VIMSS) compare
b0623 cspE -1.1 -2.4 cold shock protein E (NCBI) compare
b0678 nagB -1.1 -3.9 glucosamine-6-phosphate deaminase (NCBI) compare
b2910 zapA -1.1 -6.8 protein that localizes to the cytokinetic ring (NCBI) compare
b0738 tolR -1.1 -3.0 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b2947 gshB -1.1 -6.1 glutathione synthetase (NCBI) compare
b2892 recJ -1.1 -6.0 ssDNA exonuclease, 5' --> 3'-specific (NCBI) compare
b3726 pstA -1.1 -5.7 phosphate transporter subunit (NCBI) compare
b4594 ymgJ -1.1 -2.6 hypothetical protein (RefSeq) compare
b3120 yhaB -1.1 -1.9 orf, hypothetical protein (VIMSS) compare
b4647 mokA -1.0 -1.0 no description compare
b2699 recA -1.0 -0.7 recombinase A (NCBI) compare
b1658 purR -1.0 -4.1 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b0726 sucA -1.0 -3.1 alpha-ketoglutarate decarboxylase (NCBI) compare
b3651 trmH -1.0 -3.9 tRNA (Guanosine-2'-O-)-methyltransferase (NCBI) compare
b3149 yraO -1.0 -7.1 DnaA initiator-associating factor for replication initiation (NCBI) compare
b1361 ydaW -1.0 -0.5 orf, hypothetical protein (VIMSS) compare
b1855 lpxM -1.0 -2.9 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b4171 miaA -1.0 -3.3 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b2820 recB -1.0 -0.7 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b1712 ihfA -1.0 -1.1 integration host factor subunit alpha (NCBI) compare
b3465 yhhF -1.0 -6.0 predicted methyltransferase (NCBI) compare
b4571 wbbL -1.0 -3.9 no description compare
b0585 fes -0.9 -4.8 enterobactin/ferric enterobactin esterase (NCBI) compare
b4201 priB -0.9 -2.3 primosomal replication protein N (NCBI) compare
b4243 yjgF -0.9 -5.0 orf, hypothetical protein (VIMSS) compare
b3082 ygjM -0.9 -2.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b3649 rpoZ -0.9 -3.2 DNA-directed RNA polymerase subunit omega (NCBI) compare
b0472 recR -0.9 -5.5 recombination protein RecR (NCBI) compare
b1323 tyrR -0.9 -3.5 DNA-binding transcriptional dual regulator, tyrosine-binding (NCBI) compare
b3617 kbl -0.9 -6.0 2-amino-3-ketobutyrate coenzyme A ligase (NCBI) compare
b0949 uup -0.9 -5.2 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b4042 dgkA -0.9 -2.0 diacylglycerol kinase (NCBI) compare
b4178 yjeB -0.9 -3.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b3349 slyD -0.9 -4.6 FKBP-type peptidyl prolyl cis-trans isomerase (rotamase) (NCBI) compare
b0677 nagA -0.9 -4.4 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b1847 yebF -0.9 -1.3 predicted protein (RefSeq) compare
b4172 hfq -0.9 -1.1 RNA-binding protein Hfq (NCBI) compare
b2029 gnd -0.9 -5.2 6-phosphogluconate dehydrogenase (NCBI) compare
b1920 fliY -0.9 -1.7 cystine transporter subunit (NCBI) compare
b4414 tff -0.9 -1.2 unknown RNA (NCBI) compare
b0167 glnD -0.9 -4.1 PII uridylyl-transferase (NCBI) compare
b0908 aroA -0.8 -5.8 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b0469 apt -0.8 -5.5 adenine phosphoribosyltransferase (NCBI) compare
b4235 pmbA -0.8 -7.3 predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 (NCBI) compare
b1671 ydhX -0.8 -2.2 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b2553 glnB -0.8 -4.0 regulatory protein P-II for glutamine synthetase (NCBI) compare
b2007 yeeX -0.8 -1.3 conserved protein (RefSeq) compare
b2168 fruK -0.8 -1.0 1-phosphofructokinase (NCBI) compare
b0386 proC -0.8 -3.7 pyrroline-5-carboxylate reductase (NCBI) compare
b3244 tldD -0.8 -6.7 predicted peptidase (NCBI) compare
b3650 spoT -0.8 -4.0 bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI) compare
b3210 arcB -0.8 -4.0 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b3247 cafA -0.8 -6.3 bundles of cytoplasmic filaments (VIMSS) compare
b4553 ysaB -0.8 -4.4 hypothetical protein (NCBI) compare
b3700 recF -0.8 -6.3 recombination protein F (NCBI) compare
b3006 exbB -0.8 -5.0 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b3021 ygiT -0.8 -3.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b4431 rprA -0.8 -2.3 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b0758 galT -0.8 -3.1 galactose-1-phosphate uridylyltransferase (NCBI) compare
b2948 yqgE -0.8 -1.3 orf, hypothetical protein (VIMSS) compare
b0741 pal -0.8 -2.7 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b0957 ompA -0.8 -2.5 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b4458 oxyS -0.8 -1.8 global regulatory RNA (NCBI) compare
b4384 deoD -0.8 -5.7 purine nucleoside phosphorylase (NCBI) compare
b0143 pcnB -0.8 -5.3 poly(A) polymerase I (VIMSS) compare
b1025 ycdT -0.7 -1.3 predicted diguanylate cyclase (NCBI) compare
b1709 btuD -0.7 -0.6 vitamin B12-transporter ATPase (NCBI) compare
b1346 ydaQ -0.7 -1.4 orf, hypothetical protein (VIMSS) compare
b0685 ybfE -0.7 -3.4 orf, hypothetical protein (VIMSS) compare
b3289 rsmB -0.7 -5.7 16S rRNA m5C967 methyltransferase, S-adenosyl-L-methionine-dependent (NCBI) compare
b1984 asnW -0.7 -1.0 tRNA-Asn (NCBI) compare
b0216 aspV -0.7 -2.8 tRNA-Asp (NCBI) compare
b4383 deoB -0.7 -5.5 phosphopentomutase (NCBI) compare
b4203 rplI -0.7 -2.9 50S ribosomal protein L9 (NCBI) compare
b3163 nlpI -0.7 -3.0 hypothetical protein (NCBI) compare
b2424 cysU -0.7 -1.6 sulfate/thiosulfate transporter subunit (NCBI) compare
b1086 rluC -0.7 -4.0 23S rRNA pseudouridylate synthase (NCBI) compare


Specific Phenotypes

None in this experiment