Experiment set60S295 for Escherichia coli BW25113

Compare to:

TN_18CS_warm37C_5dbl_rep1

200 most important genes:

  gene name fitness t score description  
b0920 ycbC -8.2 -8.0 conserved inner membrane protein (NCBI) compare
b3871 bipA -6.2 -21.8 GTP-binding protein (NCBI) compare
b3341 rpsG -6.1 -3.8 30S ribosomal protein S7 (NCBI) compare
b1176 minC -5.1 -6.1 septum formation inhibitor (NCBI) compare
b3390 aroK -4.8 -3.3 shikimate kinase I (NCBI) compare
b3162 deaD -4.6 -7.7 inducible ATP-independent RNA helicase (VIMSS) compare
b1293 sapB -4.3 -3.0 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0439 lon -4.0 -6.7 DNA-binding ATP-dependent protease La (NCBI) compare
b3636 rpmG -4.0 -4.1 50S ribosomal protein L33 (NCBI) compare
b3164 pnp -3.9 -7.5 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b4234 yjgA -3.9 -13.1 hypothetical protein (NCBI) compare
b0049 apaH -3.8 -6.1 diadenosinetetraphosphatase (NCBI) compare
b2620 smpB -3.6 -2.8 SsrA-binding protein (NCBI) compare
b3652 recG -3.6 -16.8 ATP-dependent DNA helicase (NCBI) compare
b3229 sspA -3.5 -6.5 stringent starvation protein A (NCBI) compare
b3261 fis -3.3 -2.2 DNA-binding protein Fis (NCBI) compare
b2479 gcvR -3.1 -8.5 transcriptional regulation of gcv operon (VIMSS) compare
b0014 dnaK -3.1 -3.8 molecular chaperone DnaK (NCBI) compare
b3204 ptsN -3.1 -8.6 sugar-specific enzyme IIA component of PTS (NCBI) compare
b3979 thrT -3.1 -1.1 tRNA-Thr (NCBI) compare
b1175 minD -3.1 -5.2 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b2576 srmB -3.0 -6.0 ATP-dependent RNA helicase (NCBI) compare
b0149 mrcB -3.0 -17.8 penicillin-binding protein 1b (NCBI) compare
b2531 iscR -2.9 -2.5 DNA-binding transcriptional repressor (NCBI) compare
b2498 upp -2.9 -5.4 uracil phosphoribosyltransferase (VIMSS) compare
b4202 rpsR -2.9 -3.3 30S ribosomal protein S18 (NCBI) compare
b0888 trxB -2.9 -4.3 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b0051 ksgA -2.9 -7.7 dimethyladenosine transferase (NCBI) compare
b3619 rfaD -2.8 -5.3 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3493 pitA -2.8 -14.4 phosphate transporter, low-affinity (NCBI) compare
b2937 speB -2.7 -10.2 agmatinase (NCBI) compare
b3183 obgE -2.7 -3.1 GTPase involved in cell partioning and DNA repair (NCBI) compare
b2912 ygfA -2.6 -5.0 putative ligase (VIMSS) compare
b0683 fur -2.6 -2.2 ferric uptake regulator (NCBI) compare
b1290 sapF -2.5 -2.4 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2350 yfdG -2.5 -4.0 CPS-53 (KpLE1) prophage; bactoprenol-linked glucose translocase (flippase) (NCBI) compare
b4375 prfC -2.5 -1.7 peptide chain release factor 3 (NCBI) compare
b2330 yfcB -2.4 -1.6 putative adenine-specific methylase (VIMSS) compare
b0912 ihfB -2.4 -6.2 integration host factor subunit beta (NCBI) compare
b1676 pykF -2.4 -5.4 pyruvate kinase (NCBI) compare
b3621 rfaC -2.4 -7.6 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b2168 fruK -2.3 -1.8 1-phosphofructokinase (NCBI) compare
b3792 wzxE -2.3 -11.3 O-antigen translocase (NCBI) compare
b3609 secB -2.3 -2.2 export protein SecB (NCBI) compare
b3052 rfaE -2.3 -6.6 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3706 trmE -2.2 -7.4 tRNA modification GTPase (NCBI) compare
b3868 glnG -2.1 -7.9 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b3414 gntY -2.0 -7.6 predicted gluconate transport associated protein (NCBI) compare
b1095 fabF -2.0 -7.8 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b3613 yibP -2.0 -3.8 putative membrane protein (VIMSS) compare
b1863 ruvC -2.0 -2.6 Holliday junction resolvase (NCBI) compare
b0082 mraW -2.0 -4.2 S-adenosyl-methyltransferase (NCBI) compare
b3781 trxA -2.0 -6.5 thioredoxin 1 (VIMSS) compare
b4146 yjeK -1.9 -2.3 predicted lysine aminomutase (NCBI) compare
b3290 trkA -1.9 -1.9 potassium transporter peripheral membrane component (NCBI) compare
b3728 pstS -1.9 -5.9 phosphate transporter subunit (NCBI) compare
b1865 nudB -1.9 -2.4 dATP pyrophosphohydrolase (NCBI) compare
b1292 sapC -1.9 -3.0 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4685 yrbN -1.9 -5.7 expressed protein (RefSeq) compare
b3863 polA -1.9 -1.7 DNA polymerase I (NCBI) compare
b0406 tgt -1.8 -8.7 queuine tRNA-ribosyltransferase (NCBI) compare
b3169 nusA -1.8 -5.3 transcription elongation factor NusA (NCBI) compare
b1187 fadR -1.8 -3.0 fatty acid metabolism regulator (NCBI) compare
b3500 gor -1.8 -3.1 glutathione reductase (NCBI) compare
b3916 pfkA -1.8 -5.3 6-phosphofructokinase (NCBI) compare
b1860 ruvB -1.8 -1.2 Holliday junction DNA helicase B (NCBI) compare
b2830 nudH -1.8 -2.4 dinucleoside polyphosphate hydrolase (NCBI) compare
b0737 tolQ -1.8 -3.6 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1236 galU -1.7 -3.0 glucose-1-phosphate uridylyltransferase (NCBI) compare
b2569 lepA -1.7 -5.8 GTP-binding protein LepA (NCBI) compare
b2567 rnc -1.7 -1.1 ribonuclease III (NCBI) compare
b3811 xerC -1.7 -1.2 site-specific tyrosine recombinase XerC (NCBI) compare
b2501 ppk -1.7 -8.5 polyphosphate kinase (NCBI) compare
b4402 yjjY -1.7 -2.0 hypothetical protein (NCBI) compare
b3616 tdh -1.7 -7.9 L-threonine 3-dehydrogenase (NCBI) compare
b3741 gidA -1.6 -4.9 glucose-inhibited division protein A (NCBI) compare
b1286 rnb -1.6 -2.9 exoribonuclease II (NCBI) compare
b2846 yqeH -1.6 -1.0 orf, hypothetical protein (VIMSS) compare
b0759 galE -1.6 -4.4 UDP-galactose-4-epimerase (VIMSS) compare
b0741 pal -1.6 -5.2 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b0088 murD -1.6 -1.1 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b3849 trkH -1.6 -11.4 potassium transporter (NCBI) compare
b0630 lipB -1.6 -1.1 protein of lipoate biosynthesis (VIMSS) compare
b4201 priB -1.6 -3.0 primosomal replication protein N (NCBI) compare
b3198 kdsC -1.6 -4.5 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b3961 oxyR -1.6 -9.6 DNA-binding transcriptional dual regulator (NCBI) compare
b2814 metZ -1.6 -2.3 tRNA-Met (NCBI) compare
b3026 qseC -1.5 -5.4 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b2688 gshA -1.5 -8.5 glutamate--cysteine ligase (NCBI) compare
b3237 argR -1.5 -5.3 arginine repressor (NCBI) compare
b3839 tatC -1.5 -7.0 TatABCE protein translocation system subunit (NCBI) compare
b0200 gmhB -1.5 -1.0 hypothetical protein (NCBI) compare
b3620 rfaF -1.5 -5.4 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b0767 ybhE -1.4 -4.4 6-phosphogluconolactonase (NCBI) compare
b3035 tolC -1.4 -8.3 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b0890 ftsK -1.4 -2.7 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b0222 lpcA -1.4 -2.1 phosphoheptose isomerase (NCBI) compare
b2318 truA -1.4 -3.0 tRNA pseudouridine synthase A (NCBI) compare
b3205 yhbJ -1.3 -5.3 hypothetical protein (NCBI) compare
b1556 b1556 -1.3 -2.3 orf, hypothetical protein (VIMSS) compare
b2363 yfdT -1.3 -3.0 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b2892 recJ -1.3 -7.0 ssDNA exonuclease, 5' --> 3'-specific (NCBI) compare
b4208 cycA -1.2 -8.5 D-alanine/D-serine/glycine transporter (NCBI) compare
b4180 rlmB -1.2 -6.8 23S rRNA (Gm2251)-methyltransferase (NCBI) compare
b0053 surA -1.2 -5.2 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b2518 ndk -1.2 -1.7 nucleoside diphosphate kinase (NCBI) compare
b0740 tolB -1.2 -4.8 translocation protein TolB precursor (NCBI) compare
b1480 sra -1.2 -1.7 30S ribosomal subunit protein S22 (NCBI) compare
b1861 ruvA -1.2 -1.1 Holliday junction DNA helicase motor protein (NCBI) compare
b2935 tktA -1.2 -1.6 transketolase 1, thiamin-binding (NCBI) compare
b0226 dinJ -1.2 -1.5 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b3816 corA -1.2 -8.5 magnesium/nickel/cobalt transporter (NCBI) compare
b0738 tolR -1.2 -3.4 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1323 tyrR -1.2 -5.4 DNA-binding transcriptional dual regulator, tyrosine-binding (NCBI) compare
b0623 cspE -1.2 -2.8 cold shock protein E (NCBI) compare
b2297 pta -1.1 -0.8 phosphate acetyltransferase (NCBI) compare
b4179 vacB -1.1 -5.4 putative enzyme (VIMSS) compare
b0386 proC -1.1 -5.3 pyrroline-5-carboxylate reductase (NCBI) compare
b1730 ydjO -1.1 -0.9 predicted protein (RefSeq) compare
b3778 rep -1.1 -6.2 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b1269 rluB -1.1 -2.4 23S rRNA pseudouridylate synthase (NCBI) compare
b1658 purR -1.1 -4.2 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b1524 yneH -1.1 -1.1 predicted glutaminase (NCBI) compare
b3149 yraO -1.1 -5.7 DnaA initiator-associating factor for replication initiation (NCBI) compare
b4177 purA -1.1 -1.6 adenylosuccinate synthetase (NCBI) compare
b2953 yggU -1.1 -4.0 orf, hypothetical protein (VIMSS) compare
b1671 ydhX -1.1 -2.4 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b3082 ygjM -1.0 -2.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b2699 recA -1.0 -0.7 recombinase A (NCBI) compare
b2007 yeeX -1.0 -1.9 conserved protein (RefSeq) compare
b3617 kbl -1.0 -6.6 2-amino-3-ketobutyrate coenzyme A ligase (NCBI) compare
b4243 yjgF -1.0 -4.8 orf, hypothetical protein (VIMSS) compare
b3651 trmH -1.0 -4.2 tRNA (Guanosine-2'-O-)-methyltransferase (NCBI) compare
b2820 recB -1.0 -0.7 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b0605 ahpC -1.0 -2.3 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b2340 sixA -1.0 -3.3 phosphohistidine phosphatase (NCBI) compare
b0677 nagA -0.9 -3.7 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b1361 ydaW -0.9 -0.5 orf, hypothetical protein (VIMSS) compare
b1304 pspA -0.9 -2.9 regulatory protein for phage-shock-protein operon (NCBI) compare
b1105 ycfM -0.9 -2.7 predicted outer membrane lipoprotein (NCBI) compare
b3465 yhhF -0.9 -5.2 predicted methyltransferase (NCBI) compare
b1557 cspB -0.9 -1.5 Qin prophage; cold shock protein (NCBI) compare
b1984 asnW -0.9 -1.2 tRNA-Asn (NCBI) compare
b4383 deoB -0.9 -7.0 phosphopentomutase (NCBI) compare
b3163 nlpI -0.9 -2.5 hypothetical protein (NCBI) compare
b0949 uup -0.9 -5.1 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b3870 glnA -0.9 -2.0 glutamine synthetase (NCBI) compare
b3869 glnL -0.9 -5.0 sensory histidine kinase in two-component regulatory system with GlnG (NCBI) compare
b1936 intG -0.9 -1.0 predicted defective phage integrase (pseudogene) (RefSeq) compare
b0237 pepD -0.9 -5.5 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b3726 pstA -0.9 -4.0 phosphate transporter subunit (NCBI) compare
b4384 deoD -0.9 -5.9 purine nucleoside phosphorylase (NCBI) compare
b3650 spoT -0.9 -4.0 bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI) compare
b0678 nagB -0.8 -2.5 glucosamine-6-phosphate deaminase (NCBI) compare
b0472 recR -0.8 -4.6 recombination protein RecR (NCBI) compare
b1382 ynbE -0.8 -1.5 predicted lipoprotein (NCBI) compare
b3006 exbB -0.8 -4.9 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b1438 ydcQ -0.8 -3.3 predicted DNA-binding transcriptional regulator (RefSeq) compare
b3005 exbD -0.8 -2.9 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b1048 mdoG -0.8 -3.7 glucan biosynthesis protein, periplasmic (NCBI) compare
b0469 apt -0.8 -5.0 adenine phosphoribosyltransferase (NCBI) compare
b1558 cspF -0.8 -2.3 Qin prophage; cold shock protein (NCBI) compare
b1360 ydaV -0.8 -2.7 Rac prophage; predicted DNA replication protein (NCBI) compare
b0142 folK -0.8 -0.5 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b4235 pmbA -0.8 -5.9 predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 (NCBI) compare
b0167 glnD -0.8 -3.5 PII uridylyl-transferase (NCBI) compare
b1245 oppC -0.8 -4.3 oligopeptide transporter subunit (NCBI) compare
b4239 treC -0.8 -1.9 trehalose-6-P hydrolase (NCBI) compare
b4178 yjeB -0.8 -3.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b4553 ysaB -0.8 -4.6 hypothetical protein (NCBI) compare
b3761 trpT -0.8 -1.9 tRNA-Trp (NCBI) compare
b3244 tldD -0.8 -6.0 predicted peptidase (NCBI) compare
b0585 fes -0.7 -4.1 enterobactin/ferric enterobactin esterase (NCBI) compare
b0658 ybeX -0.7 -1.5 predicteed ion transport (NCBI) compare
b0908 aroA -0.7 -4.9 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b1855 lpxM -0.7 -2.7 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b3700 recF -0.7 -6.1 recombination protein F (NCBI) compare
b3935 priA -0.7 -1.9 primosome assembly protein PriA (NCBI) compare
b2947 gshB -0.7 -4.0 glutathione synthetase (NCBI) compare
b3021 ygiT -0.7 -2.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b2565 recO -0.7 -5.1 DNA repair protein RecO (NCBI) compare
b0627 tatE -0.7 -2.0 twin arginine translocase protein E (NCBI) compare
b0143 pcnB -0.7 -4.6 poly(A) polymerase I (VIMSS) compare
b0999 cbpM -0.7 -1.9 modulator of CbpA co-chaperone (NCBI) compare
b2948 yqgE -0.7 -1.0 orf, hypothetical protein (VIMSS) compare
b1248 b1248 -0.7 -2.1 orf, hypothetical protein (VIMSS) compare
b2436 hemF -0.7 -3.1 coproporphyrinogen III oxidase (NCBI) compare
b0113 pdhR -0.7 -4.1 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b4171 miaA -0.7 -2.7 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b1324 tpx -0.7 -3.1 thiol peroxidase (NCBI) compare
b4699 fnrS -0.7 -0.7 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b0726 sucA -0.7 -2.0 alpha-ketoglutarate decarboxylase (NCBI) compare
b3977 tyrU -0.7 -0.9 tRNA-Tyr (NCBI) compare
b0428 cyoE -0.7 -3.7 protoheme IX farnesyltransferase (NCBI) compare
b2329 aroC -0.7 -3.5 chorismate synthase (NCBI) compare
b1251 yciI -0.7 -2.3 orf, hypothetical protein (VIMSS) compare
b3386 rpe -0.7 -0.5 ribulose-phosphate 3-epimerase (NCBI) compare
b4511 ybdZ -0.7 -1.6 hypothetical protein (NCBI) compare
b4049 dusA -0.7 -4.6 tRNA-dihydrouridine synthase A (RefSeq) compare
b0506 allR -0.7 -2.4 DNA-binding transcriptional repressor (NCBI) compare


Specific Phenotypes

None in this experiment