Experiment set5S522 for Escherichia coli ECOR27

Bas50

Group: phage
Media: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: Ecoli_ECOR27_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 11/21/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 10 genes in this experiment

For phage Phage in Escherichia coli ECOR27

For phage Phage across organisms

SEED Subsystems

Subsystem	#Specific
Deoxyribose and Deoxynucleoside Catabolism	1
Folate Biosynthesis	1
Glycine and Serine Utilization	1
One-carbon metabolism by tetrahydropterines	1
Pyridoxin (Vitamin B6) Biosynthesis	1
Pyruvate Alanine Serine Interconversions	1
Serine-glyoxylate cycle	1
Threonine anaerobic catabolism gene cluster	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Alanine and aspartate metabolism
• Histidine metabolism
• beta-Alanine metabolism
• Fructose and mannose metabolism
• Urea cycle and metabolism of amino groups
• Glutamate metabolism
• Valine, leucine and isoleucine biosynthesis
• Arginine and proline metabolism
• Glutathione metabolism
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Keratan sulfate biosynthesis
• Lipopolysaccharide biosynthesis
• Peptidoglycan biosynthesis
• Glycerolipid metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• One carbon pool by folate
• Carbon fixation in photosynthetic organisms
• Vitamin B6 metabolism
• Carotenoid biosynthesis - General
• Zeatin biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Flavone and flavonol biosynthesis
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-alanine degradation III	1	1	1
L-alanine biosynthesis II	1	1	1
superpathway of L-alanine biosynthesis	4	4	2
L-alanine biosynthesis I	2	2	1
glutathione degradation (DUG pathway)	2	2	1
L-alanine degradation V (oxidative Stickland reaction)	2	2	1
L-alanine degradation II (to D-lactate)	3	2	1
pyruvate fermentation to acetate and alanine	3	2	1
muropeptide degradation	4	4	1
enterobacterial common antigen biosynthesis	5	4	1
C4 photosynthetic carbon assimilation cycle, NAD-ME type	11	8	2
γ-glutamyl cycle	6	5	1
L-alanine degradation VI (reductive Stickland reaction)	6	2	1
pyridoxal 5'-phosphate biosynthesis I	7	7	1
anaerobic energy metabolism (invertebrates, cytosol)	7	5	1
C4 photosynthetic carbon assimilation cycle, PEPCK type	14	9	2
folate transformations III (E. coli)	9	9	1
superpathway of L-alanine fermentation (Stickland reaction)	9	5	1
superpathway of enterobacterial common antigen biosynthesis	10	9	1
folate transformations II (plants)	11	10	1
superpathway of pyridoxal 5'-phosphate biosynthesis and salvage	12	12	1
folate transformations I	13	9	1
superpathway of anaerobic energy metabolism (invertebrates)	17	12	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	22	1