Experiment set5S521 for Escherichia coli ECOR27

Bas50

Group: phage
Media: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: Ecoli_ECOR27_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 11/21/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 12 genes in this experiment

For phage Phage in Escherichia coli ECOR27

For phage Phage across organisms

SEED Subsystems

Subsystem	#Specific
D-Galacturonate and D-Glucuronate Utilization	1
DNA-replication	1
Glycine and Serine Utilization	1
Lipid A-Ara4N pathway ( Polymyxin resistance )	1
Pyruvate Alanine Serine Interconversions	1
Teichuronic acid biosynthesis	1
Threonine anaerobic catabolism gene cluster	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose and glucuronate interconversions
• Fructose and mannose metabolism
• Ascorbate and aldarate metabolism
• Glycine, serine and threonine metabolism
• Starch and sucrose metabolism
• Nucleotide sugars metabolism
• Lipopolysaccharide biosynthesis
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Ubiquinone and menaquinone biosynthesis
• C21-Steroid hormone metabolism
• Purine metabolism
• Pyrimidine metabolism
• Cysteine metabolism
• Histidine metabolism
• Tyrosine metabolism
• Bisphenol A degradation
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Polyketide sugar unit biosynthesis
• Aminosugars metabolism
• Keratan sulfate biosynthesis
• Peptidoglycan biosynthesis
• Glycerolipid metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Linoleic acid metabolism
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Tetrachloroethene degradation
• Naphthalene and anthracene degradation
• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Folate biosynthesis
• Retinol metabolism
• Porphyrin and chlorophyll metabolism
• Zeatin biosynthesis
• Phenylpropanoid biosynthesis
• Flavone and flavonol biosynthesis
• Drug metabolism - other enzymes
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide products

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-serine degradation	3	3	3
UDP-N-acetyl-α-D-mannosaminouronate biosynthesis	1	1	1
UDP-α-D-glucuronate biosynthesis (from UDP-glucose)	1	1	1
L-tryptophan degradation II (via pyruvate)	3	3	2
pyrimidine ribonucleosides salvage I	3	3	2
D-serine degradation	3	3	2
L-cysteine degradation II	3	2	2
UDP-α-D-xylose biosynthesis	2	1	1
glycine betaine degradation III	7	4	3
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis	5	2	2
glycine betaine degradation I	8	4	3
glycine degradation	3	3	1
L-methionine biosynthesis II	6	5	2
pectin degradation I	3	1	1
L-mimosine degradation	8	4	2
glutathione-mediated detoxification I	8	3	2
superpathway of pyrimidine ribonucleosides salvage	10	10	2
superpathway of enterobacterial common antigen biosynthesis	10	9	2
enterobacterial common antigen biosynthesis	5	4	1
pectin degradation II	5	3	1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis	6	4	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	2
purine nucleobases degradation II (anaerobic)	24	17	3
teichuronic acid biosynthesis (B. subtilis 168)	9	2	1
UDP-sugars interconversion	9	2	1
nucleoside and nucleotide degradation (archaea)	10	4	1
colanic acid building blocks biosynthesis	11	11	1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis	24	7	1