Experiment set5S501 for Escherichia coli ECOR27
Bas66
Group: phageMedia: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: Ecoli_ECOR27_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 11/21/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 7 genes in this experiment
For phage Phage in Escherichia coli ECOR27
For phage Phage across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Choline and Betaine Uptake and Betaine Biosynthesis | 2 |
| Folate Biosynthesis | 1 |
| One-carbon metabolism by tetrahydropterines | 1 |
| Orphan regulatory proteins | 1 |
| Serine-glyoxylate cycle | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| folate transformations III (E. coli) | 9 | 9 | 1 |
| folate transformations II (plants) | 11 | 10 | 1 |
| folate transformations I | 13 | 9 | 1 |