Experiment set5S490 for Escherichia coli ECOR27

Bas65

Group: phage
Media: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: Ecoli_ECOR27_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 11/21/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 54 genes in this experiment

For phage Phage in Escherichia coli ECOR27

For phage Phage across organisms

SEED Subsystems

Subsystem	#Specific
Flagellum	4
Choline and Betaine Uptake and Betaine Biosynthesis	2
Orphan regulatory proteins	2
Terminal cytochrome O ubiquinol oxidase	2
Terminal cytochrome oxidases	2
Bacterial Cell Division	1
D-Galacturonate and D-Glucuronate Utilization	1
DNA repair, UvrABC system	1
Folate Biosynthesis	1
Glycine and Serine Utilization	1
Maltose and Maltodextrin Utilization	1
Molybdenum cofactor biosynthesis	1
One-carbon metabolism by tetrahydropterines	1
Pyruvate Alanine Serine Interconversions	1
RNA processing and degradation, bacterial	1
Serine-glyoxylate cycle	1
Siderophore Enterobactin	1
Threonine anaerobic catabolism gene cluster	1
Transport of Molybdenum	1
Type III secretion system orphans	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Starch and sucrose metabolism
• Nucleotide sugars metabolism
• Pentose and glucuronate interconversions
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Aminophosphonate metabolism
• Glutathione metabolism
• High-mannose type N-glycan biosynthesis
• Aminosugars metabolism
• Glycosaminoglycan degradation
• Glycerophospholipid metabolism
• Ether lipid metabolism
• Sphingolipid metabolism
• One carbon pool by folate
• Porphyrin and chlorophyll metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
cellulose biosynthesis	1	1	1
siroheme biosynthesis	4	4	2
glutathione degradation (DUG pathway)	2	2	1
pectin degradation I	3	1	1
factor 430 biosynthesis	7	3	2
pectin degradation II	5	3	1
enterobactin biosynthesis	11	7	2
γ-glutamyl cycle	6	5	1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)	13	2	2
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)	15	3	2
folate transformations III (E. coli)	9	9	1
peptidoglycan recycling II	10	7	1
folate transformations II (plants)	11	10	1
folate transformations I	13	9	1
peptidoglycan recycling I	14	14	1
adenosylcobalamin biosynthesis II (aerobic)	33	17	2
adenosylcobalamin biosynthesis I (anaerobic)	36	16	2
superpathway of chorismate metabolism	59	54	2