Experiment set5S489 for Escherichia coli ECOR27

Bas65

Group: phage
Media: LB_plus_SM_buffer + Phage (10 MOI)
Culturing: Ecoli_ECOR27_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 11/21/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 52 genes in this experiment

For phage Phage in Escherichia coli ECOR27

For phage Phage across organisms

SEED Subsystems

Subsystem	#Specific
Choline and Betaine Uptake and Betaine Biosynthesis	3
Flagellum	3
Orphan regulatory proteins	3
Terminal cytochrome O ubiquinol oxidase	2
Terminal cytochrome oxidases	2
D-Galacturonate and D-Glucuronate Utilization	1
DNA repair, UvrABC system	1
DNA structural proteins, bacterial	1
Folate Biosynthesis	1
Glycine and Serine Utilization	1
Maltose and Maltodextrin Utilization	1
One-carbon metabolism by tetrahydropterines	1
Pentose phosphate pathway	1
Peptidoglycan Biosynthesis	1
Polyamine Metabolism	1
Pyruvate Alanine Serine Interconversions	1
Serine-glyoxylate cycle	1
Siderophore Enterobactin	1
Threonine anaerobic catabolism gene cluster	1
Type III secretion system orphans	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ubiquinone and menaquinone biosynthesis
• Starch and sucrose metabolism
• Nucleotide sugars metabolism
• Peptidoglycan biosynthesis
• Porphyrin and chlorophyll metabolism
• Pentose phosphate pathway
• Pentose and glucuronate interconversions
• Ascorbate and aldarate metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Glutathione metabolism
• Glycosaminoglycan degradation
• Lipopolysaccharide biosynthesis
• Naphthalene and anthracene degradation
• One carbon pool by folate
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of siderophore group nonribosomal peptides
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
cellulose biosynthesis	1	1	1
siroheme biosynthesis	4	4	2
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
pentose phosphate pathway (oxidative branch) I	3	3	1
pectin degradation I	3	1	1
factor 430 biosynthesis	7	3	2
polyisoprenoid biosynthesis (E. coli)	5	5	1
pectin degradation II	5	3	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	6	2
juvenile hormone III biosynthesis I	6	1	1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)	13	2	2
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)	15	3	2
pentose phosphate pathway	8	8	1
folate transformations III (E. coli)	9	9	1
peptidoglycan recycling II	10	7	1
folate transformations II (plants)	11	10	1
folate transformations I	13	9	1
peptidoglycan recycling I	14	14	1
adenosylcobalamin biosynthesis II (aerobic)	33	17	2
superpathway of glucose and xylose degradation	17	17	1
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	1
peptidoglycan biosynthesis II (staphylococci)	17	12	1
adenosylcobalamin biosynthesis I (anaerobic)	36	16	2